Prex2 (NM_001107899) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Depdc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205226 representing NM_001107899 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGGAGTCCTAATGAAAATTTCTTCCGGAAATATTCAAGAAAGGGTGTTTTTTCTTTTTGATAATC TTTTGGTATACTGCAAAAGAAAACACAGACGGCTGAAGAATAGCAAGGCATCCACGGATGGGCATCGGTA TGTCTTCCGAGGGCGGATCAATACCGAGGTGATGGAAGTGGAGAATGTGGATGACGGTACAGCTGATTTT CACAGCAGTGGGCACATAGTTGTGAATGGGTGGAAGATACACAACACAGCGAAAAATAAATGGTTCGTAT GCATGGCTAAAAGCCCTGAAGAAAAGCATGAATGGTTTGAAGCTATCTTGAAAGAAAGAGAACGTCGGAA AGGTTTAAAATTAGGAATGGAGCAAGATACCTGGGTAATGATCTCCGAGCAGGGTGAGAAATTGTATAAA ATGATGTGCAAGCAGGGAAATCTGATCAAAGACAGAAAAAGAAAACTGACCACATTCCCCAAGTGCTTCC TGGGAAGTGAATTTGTCTCATGGCTGCTGGAAATTGGAGAAATCCACAGGCCTGAAGAGGGTGTTCACTT GGGACAAGCATTGCTAGAGAACGGGATCATCCACCATGTTACCGATAAGCACCAGTTCAAGCCAGAGCAA ATGCTATACCGATTCCGATATGATGATGGTACCTTTTACCCCAGGAGTGAGATGCAGGATGTGATTTCTA AGGGTGTAAGACTCTACTGTCGTCTCCATAGTCTGTTTACTCCGGTGGTCAGAGATAAAGATTATCATTT AAGAACTTACAAATCTGTCGTCATGGCCAACAAATTGATAGACTGGTTAATTGCACAGGGTGATTGCCGT ACCAGGGAAGAGGCAATGATATTTGCTGTTGGGCTTTGTGACAATGGATTCATGCATCACGTCCTAGAAA AAAGTGAATTCAAAGACGAGCCTCTACTTTTCCGCTTTTTTGCCGATGAGGAAATGGAAGGATCAAATAT GAAACACAGGCTGATGAAACATGACTTGAAGGTGGTGGAGAATGTCATAGCTAAGTCACTGTTGATTAAA TCCAATGAAGGCAGCTATGGATTTGGGCTGGAAGACAAAAATAAAGTTCCAATAATAAAGTTAGTGGAAA AAGGGTCGAATGCTGAGATGGCTGGCATGGAAGTTGGGAAGAAGATATTTGCTATTAATGGTGACCTGGT TTTTCTGAGACCTTTTCCTGAAGTTGATTGCTTCCTGAAATCGTGCTTGAACAGCAGAAAGCCACTGAGA GTCCTTGTGAGCACAAAGCCAAGGGAGACCGTTAAGATCCCAGATTCTGCAGATGGGCTTGGTTTCCAGA TCAGAGGCTTTGGTCCTTCAGTTGTACATGCTGTGGGAAGAGGTACTGTGGCTGCAGCAGCTGGCCTTCA TCCAGGACAGTGTATTATCAAAGTAAATGGAATCAACGTCAGCAAGGAGACACATGCCAGTGTCATTGCA CATGTCACCGCCTGCCGCAAATACAAGCGGCCCATGAAGCAAGATTCCATACAGTGGGTCTATGATAGTC TTGAAAGTGCTCAGGAAGACATTCAGAAGTCCCACTCAAAGCCCCCTGGAGACGAGGCTGGGGATGTTTT TGACTGCAAAGTAGAAGATGTCATGGACAAGTTCAACACCTTGGCCATTATCGACGGCAAGAAGGAGCAT GTGAGTCTGACGGTGGACAATGTCCACCTCGAGTACGGGGTTGTGTACGAGTATGACAGCACGGCTGGCA CAAAGTGCAATGTGGTGGAGAAGATGGTGGAGCCCAAAGGGTTCTTCAGCCTAACTGCCAAGATTCTGGA AGCCCTAGCAAAAAGTGATGAACATTTTGTCCAAAACTGCACCAGCCTCAATTCTTTGAATGAAGTGATC GCCACTGACCTTCAGAGTAAATTCACCAGCATGTGCAGTGAGAGAGTCGAGCATGTGTGCCACAGAATAT CCAGCTATGGGAGGTTCTCTCGGGTGCTGAAGAACAGAGCCTGGCCCACCTTTAAGCAGGCCAAAGCTAA GATGTCCCCGCTGCACAGCAGTGACTTCTGCCCTACCAACTGCCATGTGAATGTGATGGAAGTTTCTTAT CCGAAAGCATCGGCTTCCCTGGGCAGCGCCTTCGGTGTGCAGCTCGATAGTAGGAAACACAATTCCCATG ATAAGGAAAATAGGTCTGCAGACCCAGGGAAACTGAGCCCCATGGTATACATTCAACACACAATCACAAC CATGGCAGCCCCTTCAGGCCTGTCGCTGGGACACAAGGATGGCCATGGCCTCCGGTATTTGCTGAAAGAA GAAGACTTAGAAACTCAAGACATCTATCACAAACTCCTGGGCAAGCTGCAGACTGCACTGAAGGAGGTGG AGATGTGCGTTTGTCAAATCGATGACCTTCTGTCTTCAATAACATATTCTCCTAAATTGGAGCGTAAGAC AACAGAGTATGTGATACCAACGGACAGCGACAATGAAAAGGGAGAGAGAAATAGCAAACGTGTCTGTTTT AATGTAGCAGGAGATGAACAAGAGGATTCAGGTCATGATACAGTCAGCAACAGAGACTCTTACAGTGACT GCAACAGCAACAGGAATTCCATTGCCTCCTTCACGAGTATCTGCAGCAGCCAGTGCAGTTCCTACTTTCA CAGTGATGAGATGGACTCAGGTGATGAGCTTCCAACCAGCATCCGGATATCTCATGATAAACAGGACAAG ATCCACACTTGCCTGGAGCAGCTCTTCATCCAGTTGGATTCAATAACCAACCTCTTGAAAGGACGAGCTG TTGTAAGGGCCTTTGAGCAAACCAAGTATCTCACACCAGGTCGAGGATTACAAGATAGTGAAACCCAGCT CCGGAGAGACATGGTTTTCTGCCAAAGTCTTGTGGCCACTGTCTGTGCCTTCTCTGAGCAGCTCATGGCT GCCTTAAACCAGATGTTTGATAATAGCAAGGAAAATGAAATGGAGACTTGGGAAGCCAGCAGGAGGTGGC TGGACCAGATTGCAAATGCGGGTGTGCTTTTTCACTTCCAGTCACTTCTGTCACCAAATTTGAAAGATGA GCAAGCCATGCTTGAGGATACACTGGTTGCCCTGTTTGATTTGGAAAAAGTTTCCTTTTTCTTCAAACCA TCAGAAGAGGAACCACTTGTTGCAAATGTGCCTCTTACATACCAAGTGGAAGGAAGCCGGCAAGCTCTGA AAGTTGTCTTCTACATGGATAGTTACCATTTTGAGCAGCTGCCCCAACGGCTGAAGAATGGAGGCGGGTT TAAAATTCACCCTGTTCTCTTTTCACAAGCACTGGAGAGCATGGAAGGATATTGCTATAGAGACAACATC TCCGTGGAAGAGTTTCAAGCGCAGATAAACACAGCCTCCCTGGAAAAGGTCAAACAGTACAATCAGAAGC TCAGGTGTACCCTGAGTGTGACACTGGAGCAAGCCATCACTCTAGCAAGAAGCCACGGGCTCCCTCCTAG GTACATTATGCAGGCCACAGATGTGATGAGAAAGCAGGGTGCAAGAGTTCAGAACACAGCTAAGAACTTG GGGGTTAGAGACCGGACTCCTCAGTCAGCTCCGAGGTTGTATAAGCTGTGTGAGCCACCACCTCCAGTGG GAGACGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205226 representing NM_001107899 Red=Cloning site Green=Tags(s) MCGVLMKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYVFRGRINTEVMEVENVDDGTADF HSSGHIVVNGWKIHNTAKNKWFVCMAKSPEEKHEWFEAILKERERRKGLKLGMEQDTWVMISEQGEKLYK MMCKQGNLIKDRKRKLTTFPKCFLGSEFVSWLLEIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQ MLYRFRYDDGTFYPRSEMQDVISKGVRLYCRLHSLFTPVVRDKDYHLRTYKSVVMANKLIDWLIAQGDCR TREEAMIFAVGLCDNGFMHHVLEKSEFKDEPLLFRFFADEEMEGSNMKHRLMKHDLKVVENVIAKSLLIK SNEGSYGFGLEDKNKVPIIKLVEKGSNAEMAGMEVGKKIFAINGDLVFLRPFPEVDCFLKSCLNSRKPLR VLVSTKPRETVKIPDSADGLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIA HVTACRKYKRPMKQDSIQWVYDSLESAQEDIQKSHSKPPGDEAGDVFDCKVEDVMDKFNTLAIIDGKKEH VSLTVDNVHLEYGVVYEYDSTAGTKCNVVEKMVEPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVI ATDLQSKFTSMCSERVEHVCHRISSYGRFSRVLKNRAWPTFKQAKAKMSPLHSSDFCPTNCHVNVMEVSY PKASASLGSAFGVQLDSRKHNSHDKENRSADPGKLSPMVYIQHTITTMAAPSGLSLGHKDGHGLRYLLKE EDLETQDIYHKLLGKLQTALKEVEMCVCQIDDLLSSITYSPKLERKTTEYVIPTDSDNEKGERNSKRVCF NVAGDEQEDSGHDTVSNRDSYSDCNSNRNSIASFTSICSSQCSSYFHSDEMDSGDELPTSIRISHDKQDK IHTCLEQLFIQLDSITNLLKGRAVVRAFEQTKYLTPGRGLQDSETQLRRDMVFCQSLVATVCAFSEQLMA ALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHFQSLLSPNLKDEQAMLEDTLVALFDLEKVSFFFKP SEEEPLVANVPLTYQVEGSRQALKVVFYMDSYHFEQLPQRLKNGGGFKIHPVLFSQALESMEGYCYRDNI SVEEFQAQINTASLEKVKQYNQKLRCTLSVTLEQAITLARSHGLPPRYIMQATDVMRKQGARVQNTAKNL GVRDRTPQSAPRLYKLCEPPPPVGDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107899
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107899.1, NP_001101369.1
RefSeq Size	4852 bp
RefSeq ORF	3651 bp
Locus ID	312912
MW	138 kDa

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