Pitpnm1 (NM_001008369) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pitpnm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205416 representing NM_001008369 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTATCAAGGAGTACCACATCTTGCTGCCCATGAGCCTGGACGAGTACCAAGTGGCCCAGCTCTACA TGATCCAGAAAAAGAGCCGGGAGGAATCTAGTGGTGAGGGCAGTGGTGTGGAGATCCTGGCCAACCGGCC CTACACAGATGGACCCGGAGGCAATGGGCAGTACACACACAAGGTGTACCACGTGGGCTCCCACATCCCA GGCTGGTTCCGGGCACTGTTGCCCAAGGCTGCTCTGCAGGTAGAAGAGGAATCTTGGAATGCTTACCCGT ACACCCGGACACGGTACACCTGCCCCTTTGTGGAGAAATTCTCCATTGAGATAGAGACCTATTACTTGCC TGATGGGGGGCAGCAGCCTAACGTCTTCAACTTGAGCGGGGCGGAGAGAAGACAGAGAATCCTGGATACC ATCGACATCGTGCGGGATGCAGTGGCCCCAGGAGAGTACAAAGCAGAAGAGGACCCTCGGCTGTACCGCT CAGTCAAGACAGGCCGAGGGCCACTGGCTGATGACTGGGCACGGACAGCAGCCCAGACAGGACCTCTCAT GTGTGCCTATAAGCTGTGCAAGGTTGAATTCCGCTACTGGGGCATGCAGGCCAAGATTGAGCAGTTCATC CATGATGTAGGTCTGCGCAGGGTAATGCTTCGGGCCCATCGCCAGGCCTGGTGTTGGCAGGATGAGTGGA TTGAACTGAGCATGGCTGACATCCGGGCACTGGAAGAGGAGACAGCACGTATGCTGGCCCAGCGTATGGC TAAGTGCAACACTGGCAGTGAGGGACCGGAGGCCCAGACCCCTGGGAAACCCAGCACTGAGACCCAGCCT GGGACCAGAACGGCTGGCACCCCTGATGGGCCTGAGGCCCCTCCTGGCCCCGACGCCTCACCAGATGCCA GCTTTGGGAAGCAGTGGTCCTCATCTTCCCGTTCCTCCTACTCATCCCAACATGGAGGTGGTGTGTCTCC ACAGAGCTTGTCTGAGTGGCGCATGCAGAACATTGCCCGGGACTCTGAGAACAGCTCCGAGGAAGAATTC TTTGATGCCCATGAAGGTTTCTCAGACAGTGATGAGGTCTTCCCCAAGGAGATGACCAAGTGGAACTCCA ATGATTTTATTGATGCCTTTGCCTCCCCGACTGAGGTAGAGGGGGTGCCAGATCCTGCAATCATGGCTAC CAAAGGCATTGAAGATGAGGCCCGAGCTCCCAGGGACTCAGAGGGCCTGGATGGAACCGGGGATCTAGGG GTTGAGGCATGCTCTGTGCATGCCCTCTTCCTCATCCTACACAGCGGCAGCATCCTGGACTCTGGCCCTG GAGACACCAACTCCAAGCAGGCCGATGTTCAAACACTGAGCACAGCCTTTGAGGCCGTCACCCGAGTCCA TTTCCCCGAGGCCCTGGGTCACGTGGCACTGCGGCTGGTGCCCTGTCCACCCATCTGCGCGGCTGCCTAT GCTCTTGTCTCCAACCTGAGCCCCTACAGCCATGATGGGGACAGCCTGTCCCGCTCCCAGGACCACATTC CACTGGCTGCTCTGCCACTGCTGGCCACCTCATCCTCCCGCTACCAGGGTGCCGTGGCCACTGTCATCGC TCGCACCAACCAGGCCTATGCAGCCTTCCTGCGCTCATCGGAGGGCACAGGTTTCTGCGGGCAGGTGGTG CTGATCGGAGATGGTGTTGGTGGCATCCTGGGCTTTGATGCGCTCTGCCACAGTGCCAGTGCAGGCACAG GGAGTCGAGGCAGCAGCCGCCGTGGGAGCATGAACAATGAGATGCTCTCCCCAGAAGTTGGTCCAGTACG GGACCCGCTGGCAGATGGGGTGGAGGTACTGGGTCGTGCTAGCCCAGAACCCTCAGCCCTACCTGCTCAG CGCACCTCCAGCGACATGGCTAACCCGGATCCTGATGGCTCTCAGAACAGCCTACAGGTAGCCCCCACAG TTACCTCTGGGGAGCCCCGTCGTGCAAGCACAGCCTCCTGTCCACCTGCCAGTTCTGAGGCTCCTGACGG CCCCACTAATGCTGCTCGACTGGACTTCAAGGTCTCAGGCTTCTTCCTCTTTGGCTCCCCACTGGGCTTG GTGCTGGCTCTACGCAAAACCGTCATGCCTGCCTTGGAGGTGGCTCAGATGCGTCCAGCCTGCGAACAGA TCTACAATCTCTTCCATGCCGCTGACCCCTGTGCCTCCCGCCTGGAACCCCTGCTGGCCCCCAAGTTCCA GGCCATCGCCCCCCTGGCTGTACCTCGTTACCAGAAGTTTCCCCTAGGAGATGGCTCATCCCTGCTGCTG GCTGACACTTTGCAGACCCATTCGAGCCTCTTCCTGGAAGAGCTGGAGATGATGGTGCCCTCCACGCCCA CCTCGGCCAGTGGTGCCTTCTGGAAGGGCAATGAATTAGGCAATGAGCCGGCAGCCCAGCCAGCAGCCCC CAGCACCACCAGTGAGGTGGTTAAGATCCTGGATCGCTGGTGGGGAAACAAGCGCATCGACTATTCACTG TACTGCCCTGAGGCACTCACCGCCTTTCCCACCGTCACGCTGCCCCACCTCTTCCACGCCAGCTACTGGG AGTCGGCAGACGTAGTGGCCTTCATTCTGCGCCAGGTCATTGAGAAGGAGCGGCCACAGTTGACAGAATG TGAGGAGCCATCCATCTACAGCCCTGCCTTCCCCAGGGAGAAGTGGCAGCGCAAACGCACACAGGTCAAG ATCCGGAACGTCACTTCCAACCATCGGGCAAGTGACACCGTGGTATGCGAGGGCCGTCCCCAGGTGCTGA ATGGGCGCTTCATGTACGGACCACTGGACGTGGTCACACTCACTGGCGAGAAGGTGGATGTCTATGTCAT GACACAGCCCCTGTCGGGCAAGTGGATCCACTTTGGCACAGAGGTCACCAACAGCTCAGGCCGTCTCACC TTCCCAGTGCCCTCAGAACGTGCACTGGGCATTGGTGTCTACCCTGTGCGCATGGTGGTCAGGGGAGACC ATACCTACGCCGAGTGCTGTCTGACTGTGGTGTCCCGAGGCACAGAAGCTGTGGTCTTCAGCATCGACGG CTCATTCACTGCCAGTGTCTCTATCATGGGCAGTGACCCCAAGGTGCGCGCTGGCGCAGTGGACGTGGTC AGGCACTGGCAGGACTCAGGCTACTTGATCGTGTATGTAACTGGCCGGCCTGACATGCAGAAGCACCGTG TAGTGGCCTGGCTGTCGCAACACAACTTCCCCCATGGTGTTGTCTCCTTCTGTGATGGCCTCACCCATGA CCCACTGCGACAGAAAGCTATGTTTCTGCAGAGCCTGGTGCAAGAGGTAGAACTGAACATCGTGGCTGGG TATGGGTCACCGAAAGACGTTGCAGTATATGCGGCCCTGGGACTCTCTCCAAGCCAGACCTACATTGTTG GTCGTGCAGTGCGTAAACTGCAGGCACAGTGTCAGTTCCTGTCAGATGGCTATGTGGCCCATTTGGGCCA GCTGGAGGCAGGCTCCCACTCCCATGCCCCCTCAGGACCTCCAAGAGCTGCTCTGGCCAAGAGCAGCTAT GCTGTGGCTGCCCCTGTGGACTTCCTCCGTAAGCAGAGCCAGCTGCTTCGATCCAGAGGCCCCAGCCAGG TGGACCTCGAGGGCCCAGGAACACCTCCTACCACCCTGGCCAGGGGCAAGACTCGAAGCATCAGCCTCAA GCTAGACAGTGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205416 representing NM_001008369 Red=Cloning site Green=Tags(s) MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGNGQYTHKVYHVGSHIP GWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDT IDIVRDAVAPGEYKAEEDPRLYRSVKTGRGPLADDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFI HDVGLRRVMLRAHRQAWCWQDEWIELSMADIRALEEETARMLAQRMAKCNTGSEGPEAQTPGKPSTETQP GTRTAGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGGVSPQSLSEWRMQNIARDSENSSEEEF FDAHEGFSDSDEVFPKEMTKWNSNDFIDAFASPTEVEGVPDPAIMATKGIEDEARAPRDSEGLDGTGDLG VEACSVHALFLILHSGSILDSGPGDTNSKQADVQTLSTAFEAVTRVHFPEALGHVALRLVPCPPICAAAY ALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYAAFLRSSEGTGFCGQVV LIGDGVGGILGFDALCHSASAGTGSRGSSRRGSMNNEMLSPEVGPVRDPLADGVEVLGRASPEPSALPAQ RTSSDMANPDPDGSQNSLQVAPTVTSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGL VLALRKTVMPALEVAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLL ADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGNELGNEPAAQPAAPSTTSEVVKILDRWWGNKRIDYSL YCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLTECEEPSIYSPAFPREKWQRKRTQVK IRNVTSNHRASDTVVCEGRPQVLNGRFMYGPLDVVTLTGEKVDVYVMTQPLSGKWIHFGTEVTNSSGRLT FPVPSERALGIGVYPVRMVVRGDHTYAECCLTVVSRGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVV RHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAG YGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHAPSGPPRAALAKSSY AVAAPVDFLRKQSQLLRSRGPSQVDLEGPGTPPTTLARGKTRSISLKLDSEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001008369
ORF Size	3726 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001008369.1, NP_001008370.1
RefSeq Size	4191 bp
RefSeq ORF	3729 bp
Locus ID	361694
UniProt ID	Q5U2N3
MW	135 kDa
Gene Summary	Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions (By similarity).[UniProtKB/Swiss-Prot Function]

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