Dmac2 (NM_001009705) Rat Tagged ORF Clone

CAT#: RR205983

  • TrueORF®

Atp5sl (Myc-DDK-tagged ORF) - Rat ATP5S-like (Atp5sl), (10 ug)



  "NM_001009705" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Atp5sl; MGC109149
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205983 representing NM_001009705
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCCAGAGCGTTTCTGCACCTGGGTGCCCGAGAATGGCATGGGAGAGCCAGGGGCACCCACA
GCATGAGTGGGTTGGCGACCCCAGACAGCAATCGGGAGAAGAAAAGAACACTGCTGCAGTTCCTCTCAGA
CCACTTCCATGACGTCCAGACTCTGAGAGAGTACTTGCTCCAGAAACAGATCTCAAAAGTCAGCATGGAG
AATCGATCCTACAGGAAGATCCAGGAGAGATACGGCCCATACATCACAGGTGCTCAGTTCATCCTGAAGC
AGGGAGGCGCAGTGAAGTTTCAGGGCAGGGATTGGATCCGGCCAAATGACCGTGGCCATTCTATTGCCGA
GCTCCAGAAGGTGCCTGTGGAGGCTGTGGATGCCAGTGGCTGTGCCATCAACTACCAGGGCCTCAGTAAC
CTCTTGCCCCTGAAGGAGCTGCAGTTCCTGTCCCTTCAGCGCTGCCCCAACTTGGATGACTGGTGCCTCA
GCCGCCTCTACCTACTGGCTGGTTCACTGCAGGAGCTCTCTCTGGCTGGGTGCCCCAGAATCTCAGAACG
AGGCCTTGCCTGTCTCCACCACCTCCAGAACCTCCGCAGGCTGGACATCTCAGACCTCCCTGCCGTGTCC
CACCCAGGTCTTACTCAGATCCTAGTGGAAGAGATGTTGCCCCATTGTGAGGTCCTTGGAGTTGATTGGG
CCAAGAGTCTGAAGCTGGGGCCAGACGATCAACCTCCCGACACATCTAGTCCTCTCTCTTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205983 representing NM_001009705
Red=Cloning site Green=Tags(s)

MAAPRAFLHLGAREWHGRARGTHSMSGLATPDSNREKKRTLLQFLSDHFHDVQTLREYLLQKQISKVSME
NRSYRKIQERYGPYITGAQFILKQGGAVKFQGRDWIRPNDRGHSIAELQKVPVEAVDASGCAINYQGLSN
LLPLKELQFLSLQRCPNLDDWCLSRLYLLAGSLQELSLAGCPRISERGLACLHHLQNLRRLDISDLPAVS
HPGLTQILVEEMLPHCEVLGVDWAKSLKLGPDDQPPDTSSPLSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001009705
ORF Size 762 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001009705.1, NP_001009705.1
RefSeq Size 1873 bp
RefSeq ORF 765 bp
Locus ID 361520
UniProt ID Q5I0I4
MW 28.5 kDa
Gene Summary Required for the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Involved in the assembly of the distal region of complex I.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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