Atp2a3 (NM_012914) Rat Tagged ORF Clone
CAT#: RR206015
- TrueORF®
Atp2a3 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, ubiquitous (Atp2a3), (10 ug)
"NM_012914" in other vectors (3)
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CNY 16,060.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SERCA3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206015 representing NM_012914
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGCGCACCTGCTCTCGGCTGCCGACGTGCTGCGTCGCTTCTCGGTGACAGCCGAGGGCGGCT TGACCCTGGAGCAGGTGACCGACGCCCGGGAGCGATACGGCCCCAACGAACTCCCTACTGAGGAAGGGAA ATCCCTGTGGGAGCTGGTGGTGGAGCAGTTTGAGGACCTCTTGGTGCGCATCCTGCTGCTGGCAGCCCTG GTTTCCTTTGTCCTGGCCTGGTTTGAGGAGGGTGAGGAGACCACGACGGCCTTCGTGGAGCCCCTCGTCA TCATGTTAATCCTTGTGGCCAATGCGATCGTGGGAGTGTGGCAGGAACGCAATGCTGAGAGTGCCATTGA GGCCTTGAAGGAGTATGAGCCTGAGATGGGTAAGGTGATCCGCTCTGACCGCAAGGGTGTGCAGAGGATC CGAGCCAGGGACATCGTCCCTGGGGACATTGTGGAAGTGGCAGTGGGAGACAAGGTACCTGCTGACCTCC GCCTCATCGAGATCAAGTCCACCACACTTCGAGTGGACCAGTCCATCCTGACAGGGGAATCCGTGTCTGT GACTAAGCACACAGATGCCATTCCAGACCCCCGAGCCGTGAACCAGGACAAGAAGAACATGCTGTTTTCT GGCACCAATATTGCATCAGGCAAAGCCCTGGGTGTGGCTGTGGCCACAGGCTTGCACACAGAGCTGGGCA AGATTCGGAGCCAGATGGCGGCTGTGGAGCCTGAGCGGACGCCACTGCAGCGCAAGCTGGATGAATTTGG GAGGCAGCTGTCCCATGCCATCTCTGTGATCTGTGTGGCTGTGTGGGTCATCAACATCGGACACTTTGCT GACCCGGCCCATGGTGGATCCTGGCTCCGTGGGGCAGTCTACTACTTCAAGATTGCTGTGGCCCTGGCTG TGGCTGCTATCCCCGAGGGCCTCCCAGCAGTCATCACTACATGCCTAGCACTGGGCACAAGACGTATGGC ACGTAAGAACGCCATTGTGCGGAGCCTGCCCTCTGTGGAGACCCTGGGCTGCACCTCAGTCATCTGCTCT GACAAGACAGGGACACTCACCACCAATCAAATGTCTGTCTGCAGGATGTTCGTGGTAGCTGAAGCAGAGG CAGGCGCCTGTCGTTTACACGAATTCACCATTTCGGGTACCACGTATACCCCAGAGGGCGAAGTGAGGCA AGGGGAACAGCTTGTGCGCTGTGGGCAGTTTGATGGACTTGTGGAGCTCGCAACCATCTGTGCACTCTGC AATGATTCTGCACTGGACTACAATGAGGCCAAGGGTGTGTATGAGAAGGTAGGAGAGGCCACAGAGACAG CCCTCACTTGCCTGGTAGAGAAGATGAACGTGTTTGACACAGACCTGAAAGGACTGTCGCGGGTCGAGCG CGCTGGTGCCTGCAATTCCGTCATCAAGCAACTCATGCAGAAGGAGTTCACCCTCGAATTCTCCCGGGAC CGGAAGTCCATGTCTGTGTACTGCACACCCACTCGTGCTGACCCCAAGGCCCAGGGCAGCAAGATGTTTG TGAAGGGAGCTCCTGAGAGTGTAATTGAGCGCTGCAGCTCAGTCCGCGTGGGAAGCCGAACAGTACCCCT AAGCGCCACCTCCAGAGAGCATATCCTGGCCAAGATCCGAGACTGGGGCTCAGGCTCTCATACATTGCGC TGCTTAGCACTGGCCACCAGGGACACACCCCCAAGGAAGGAGGACATGCAACTGGATGACTGCAGTCAGT TTGTACAGTACGAGACAGGCCTGACCTTCGTGGGCTGCGTGGGCATGTTAGACCCACCGAGACCAGAGGT GGCTGCCTGTATCACACGCTGTTCTCGGGCGGGCATTCGAGTGGTCATGATCACAGGGGACAACAAAGGG ACAGCTGTGGCCATCTGCCGCCGACTTGGCATCTTTGGGGATACGGAGGATGTGTTGGGCAAGGCTTACA CGGGCCGAGAATTCGATGACCTCAGCCCAGAGCAGCAGCGCCAGGCTTGCCGTACCGCCCGATGCTTTGC CCGTGTGGAACCCGCACATAAGTCTCGCATCGTGGAGAACCTCCAGTCCTTTAATGAGATCACCGCCATG ACTGGCGATGGGGTGAATGATGCACCAGCCCTGAAGAAGGCAGAGATCGGCATCGCCATGGGCTCAGGCA CTGCTGTGGCCAAGTCGGCAGCGGAGATGGTTCTGTCTGATGACAACTTTGCCTCCATTGTGGCTGCAGT GGAGGAGGGCAGGGCCATCTACAACAACATGAAGCAATTCATCCGCTACCTCATCTCCTCCAATGTTGGC GAGGTTGTCTGCATCTTCCTCACAGCAATTCTGGGCCTGCCCGAAGCCCTGATCCCTGTGCAGCTGCTCT GGGTGAACCTGGTAACAGATGGCTTACCTGCCACAGCCCTTGGCTTCAACCCACCCGATCTGGACATCAT GGAAAAGCTGCCTCGGAACCCCCGGGAGGCTCTCATCAGCGGCTGGCTTTTTTTCCGCTATTTGGCTATT GGAGTGTACGTAGGCCTGGCTACAGTGGCTGCCGCCACCTGGTGGTTCCTGTATGACGCTGAGGGACCAC AAGTCACCTTCCATCAGCTGAGGAACTTCCTGAAGTGCTCTGAAGACAATCCACTGTTTGCCGGCATCGA CTGCGAGGTCTTTGAGTCACGCTTCCCCACGACCATGGCCTTATCTGTGCTTGTGACCATTGAAATGTGC AACGCCCTTAACAGCGTCTCAGAGAACCAGTCACTGCTGCGTATGCCACCCTGGCTGAACCCTTGGCTGC TGGGGGCTGTGGTCATGTCCATGGCCCTGCATTTTCTCATCCTTTTGGTGCCGCCTCTGCCCCTCATTTT CCAGGTGACCCCACTGAGTGGTCGACAGTGGGGGGTGGTGCTTCAGATGTCTCTGCCTGTCATCCTGCTT GACGAGGCCCTCAAGTATCTGTCCAGGCATCACGTGGATGAAAAAAAGGACCTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206015 representing NM_012914
Red=Cloning site Green=Tags(s) MEEAHLLSAADVLRRFSVTAEGGLTLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAAL VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFS GTNIASGKALGVAVATGLHTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS DKTGTLTTNQMSVCRMFVVAEAEAGACRLHEFTISGTTYTPEGEVRQGEQLVRCGQFDGLVELATICALC NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMQKEFTLEFSRD RKSMSVYCTPTRADPKAQGSKMFVKGAPESVIERCSSVRVGSRTVPLSATSREHILAKIRDWGSGSHTLR CLALATRDTPPRKEDMQLDDCSQFVQYETGLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKG TAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVG EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKLPRNPREALISGWLFFRYLAI GVYVGLATVAAATWWFLYDAEGPQVTFHQLRNFLKCSEDNPLFAGIDCEVFESRFPTTMALSVLVTIEMC NALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILL DEALKYLSRHHVDEKKDLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012914 |
ORF Size | 2997 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012914.1, NP_037046.1 |
RefSeq Size | 4472 bp |
RefSeq ORF | 3000 bp |
Locus ID | 25391 |
UniProt ID | P18596 |
MW | 109.4 kDa |
Gene Summary | pumps Ca+2 into the endoplasmic reticulum; plays a role in cellular Ca+2 signaling [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206015 | Atp2a3 (untagged ORF) - Rat ATPase, Ca++ transporting, ubiquitous (Atp2a3), (10 ug) |
CNY 16,060.00 |
|
RR206015L3 | Lenti ORF clone of Atp2a3 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, ubiquitous (Atp2a3), (10 ug) |
CNY 17,580.00 |
|
RR206015L4 | Lenti ORF clone of Atp2a3 (mGFP-tagged ORF) - Rat ATPase, Ca++ transporting, ubiquitous (Atp2a3), (10 ug) |
CNY 19,380.00 |