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Gpld1 (NM_001100512) Rat Tagged ORF Clone
CAT＃: RR206031
- TrueORF®
Gpld1 (Myc-DDK-tagged ORF) - Rat glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10 ug)
"NM_001100512" in other vectors (3)
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CNY 10,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206031 representing NM_001100512
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTAGGCAGGCTGTGGTCTGGCCTGCTGCTCCTGCTGCTTTTTTTCTGCTCTAGAAGTTCATCTT GTGGTCTCTCAACACATGTAGAAATAGGACACAGGGCTCTGCAGTTCCTTCAGCTTCAAGATGGACGCAT TAACTACAAAGAGCTGCTCTTAGAACACCAGGATGCATATCAGGCAGGGACGGTGTTTCCTGATGCCTTT TACCCTAGCATCTGCAAACAAGGAAAATTCCATGAAGTGTCTGAGAGTACTCACTGGACTCCATTTCTTA ATGCCAGCATTCATTATATTCGGGAGAACTACCCTCTGCCCTGGGAGAAGGACACAGAGAAGTTGGTGGC TTTCTTGTTTGGAGTCACCTCCCACATGGTCGCTGATGTCAGCTGGCACAGCCTGGGTATTGAACAAGGG TTCCTCAGGACAATGGGAGCTATTGATTTTTACGACTCTTATTCTGAGGCTCATTCGGCTGGTGATTTTG GAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTACCTCTCACGGCGCTGGTACGTGCCCATCCA AGATCTTCTGAGAATTTATGATAATCTCTACGGTCGGAAAGTCATCACCAAGAATGTCATTGTTGATTGC ACATACCTTCAGTTCCTGGAAATGCACGGGGAGATGCTAGCTGTTTCCAAGCTCTATTCCACATACTCTA CGAAGTCCCCATTTCTGGTGGAACAGTTCCAAGACTATTTCCTCGGAGGTCTAGATGACATGGCCTTCTG GTCCACGAACATTTACCGTTTGACCAGCTTTATGCTGGAGAACGGGACCAGTGACTGCAACCTGCCTGAG AACCCTCTGTTCATCTCCTGTGATGGTAGGAAGAACCACACCCTCAGCAGCTCAAAAGTGCAGAAAAATG ATTTTCACAGGAATTTGACCATGTTTATAAGTAAAGACATCAGGAAAAACCTCAACTACACAGAAAGAGG CGTGTTCTACAGCACAGGCTCCTGGGCCCCGGAATCCGTAACCTTTATGTACCAGACTCTGGAGAGGAAC CTGAGGATGATGTTTACTGGCAACTCTCAGACAGCTCTGAAGCATGTCTCCAGTCCCTCTGCCTCCTACA CCCTGTCTGTCCCCTATGCCAGGCTCGGCTGGGTCATGGCCTCAGCTGACCTCAACCAGGATGGGCACGG CGACCTCGTGGTGGGTGCACCAGGCTACAGCCACCCTGGCCGCTTCCAGATCGGGCGGGTGTACATCATC TATGGCAATGATTTGGGCCTGCCCCCCATCGACCTGGACTTAGACAAGGAGGCTCATGGGGTCCTGGAGG GCTTCCAGCCTTCAGGTCGCTTTGGCTCAGCTTTGGCAGTGCTGGACTTCAACAAGGATGGGCTGCCTGA TCTGGCTGTGGGTGCTCCCTCCGTGGGCTCAGGGCAGCTCACCTACAATGGCTCTGTGTATGTCTACTAT GGTTCCCAGCAAGGGAGGTTGTCTTCTTCCCCTAACATCACCATCTCCTGCAAGGACACCTACTGTAATT TGGGCTGGGCTCTCTTGGCAGCAGACGCAGATGGAGATGGCCAGCATGATCTGGTGATCAGTTCACCTTT TGCACCAGGTGGAGGGAAGCAGAGAGGAATTGTGGCTGCCTTTTATTCACACCCCAGACGGAACGACAAA GAGTCACTGACTTTGGACGAAGCTGACTGGAAGGTGAACGGGGAGGAGGACTTCTCATGGTTTGGATACT CCCTTCATGGGGTCACAGTGGCAAACAGAAGCCTGCTGCTGATTGGGAGCCCGACCTGGAAGAATATCAG CAGGATGGCACGCTCAAGCCACCAAAAGAACCAAGAGAAAAAGAGCCTGGGAAGGGTGTATGGCTACTTC CCCCCAAACCGGCAGAGGGAAATCACTATCTCGGGAGATAAGGCAATGGGAAAGCTGGGTACATCCCTGT CCAGTGGTTATGTGAGGGTGAATGGCACCCTAACCCAAGTCCTGCTGGTTGGAGCCCCTACTCATGATGA CGTGTCTAAAATGGCATTCCTAACCATGACCCTGCACCAGGGTGGAGCCACTCGGATGTACGAGCTAGCC CCTGAGAAGACACAGCCTGCTCTGCTCAGCACCTTCAGCGGAGACCGACGTTTTTCTCGATTTGGTAGTG TTCTACACCTGACAGACCTGGATGACGATGGCTTAGATGAAATCATCATGGCTGCCCCACTGAGGATAAC AGATGTCACTTCTGGACTGCTTGGGGGAGAAGATGGGCGAGTGTACATTTATAATGGCATGCATACCACC TTAGGTGATGTGACAGGCAAATGCAAGTCCTGGATGACTCCATGTCCAGAGGAGAAGGCCCAGTATGTGC TAATTTCTCCAGAGGCAAGTTCAAGGTTTGGGAGCTCACTGGTGTCTGTGCGGTCTAAGGAAAGGAACCA AGTGGTCGTTGCTGCTGGAAGGAGTTCTTGGGGAGCCCGGCTCTCTGGGGCACTTCACGTCTACAGCTTC AGCTCAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206031 representing NM_001100512
Red=Cloning site Green=Tags(s) MSVGRLWSGLLLLLLFFCSRSSSCGLSTHVEIGHRALQFLQLQDGRINYKELLLEHQDAYQAGTVFPDAF YPSICKQGKFHEVSESTHWTPFLNASIHYIRENYPLPWEKDTEKLVAFLFGVTSHMVADVSWHSLGIEQG FLRTMGAIDFYDSYSEAHSAGDFGGDVLSQFEFNFNYLSRRWYVPIQDLLRIYDNLYGRKVITKNVIVDC TYLQFLEMHGEMLAVSKLYSTYSTKSPFLVEQFQDYFLGGLDDMAFWSTNIYRLTSFMLENGTSDCNLPE NPLFISCDGRKNHTLSSSKVQKNDFHRNLTMFISKDIRKNLNYTERGVFYSTGSWAPESVTFMYQTLERN LRMMFTGNSQTALKHVSSPSASYTLSVPYARLGWVMASADLNQDGHGDLVVGAPGYSHPGRFQIGRVYII YGNDLGLPPIDLDLDKEAHGVLEGFQPSGRFGSALAVLDFNKDGLPDLAVGAPSVGSGQLTYNGSVYVYY GSQQGRLSSSPNITISCKDTYCNLGWALLAADADGDGQHDLVISSPFAPGGGKQRGIVAAFYSHPRRNDK ESLTLDEADWKVNGEEDFSWFGYSLHGVTVANRSLLLIGSPTWKNISRMARSSHQKNQEKKSLGRVYGYF PPNRQREITISGDKAMGKLGTSLSSGYVRVNGTLTQVLLVGAPTHDDVSKMAFLTMTLHQGGATRMYELA PEKTQPALLSTFSGDRRFSRFGSVLHLTDLDDDGLDEIIMAAPLRITDVTSGLLGGEDGRVYIYNGMHTT LGDVTGKCKSWMTPCPEEKAQYVLISPEASSRFGSSLVSVRSKERNQVVVAAGRSSWGARLSGALHVYSF SSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100512 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100512.1, NP_001093982.1 |
| RefSeq Size | 3212 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 291132 |
| UniProt ID | Q8R2H5 |
| MW | 93.7 kDa |
| Gene Summary | an enzyme that is involved in insulin signaling pathways and is regulated by insulin [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206031 | Gpld1 (untagged ORF) - Rat glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10 ug) |
CNY 10,740.00 |
|
| RR206031L3 | Lenti ORF clone of Gpld1 (Myc-DDK-tagged ORF) - Rat glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10 ug) |
CNY 12,640.00 |
|
| RR206031L4 | Lenti ORF clone of Gpld1 (mGFP-tagged ORF) - Rat glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10 ug) |
CNY 13,780.00 |
