Depdc5 (NM_001107229) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206263 representing NM_001107229 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGACTAAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACCCAGAAAGTGGAATTTGCTGGCA TCAGGGCACAGGCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGAC CAGGGTGGTGTTCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGAT TTTGATATTTATGGGGATCTGTACTTTGAGAAAGCTGTGAATGGTTTCCTTGCCGACCTGTTTACTAAGT GGAAGGAGAAGAACTGTAGCCATGAAGTGACTGTGGTTCTGTTTTCTAGAACTTTTTATGATGCAAAATC TATTGATGAATTTCCTGAAGTAAACCGAGCTTCAATTCAACAGGATCATAAAGGGAGATTCTACGAGGAC TTTTACAAAGTGGTGGTGCAGAACGAGAGAAGGGAAGAGTGGACGTCACTCCTTGTGACCATTAAGAAAC TCTTCATCCAGTATCCAGTGTTGGTTCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTAC CTCAGCACAAGGAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAAC CGAAACTTTGACCGAACTGGGCAGATGTCCGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACC GCCTCCTCATGATCTTGACCAAGCAGCGGATGATCGATAATGGAATTGGTGTGGACTTAGTGTGCATGGG AGAGCAGCCATTACACGCTGTGCCATTATTCAAGCTGCACAACCGGAGTGTGCCACGGGATTCTCGGCTG GGCGATGACTATAATATTCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTGTTTTGTA ACAGTTTCACTCCAAGGATAAAACTGGCAGGAAAGAAGTCTGCCACTGAGAAAACCAAAAATGGCCGTGA TACATCTCTTGGGGCTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGAT GCCCAAGTATTCCGGCTGCCTGGTCCATCCCGGGCCCAGCGACTCGCCACCTGCAGGTCTGTGAGAGAGC AGGAAAATCACAATCGCAAAAGTGCCAGCTCCTGTGACGTCTCCTCCAGCCCTTCCTTGCCAAGCCGTGC ACTGCCCACTGAGGAAGTGAGGAGCCAGGCTTCAGACGACAGCTCCCTGGGCAAGAGCACCAACATCCTG ATGATCCCTAACCCCCATCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATG TCCTGGAGAACATGATAGAACCACCACAGCGGGACTCTAGTGCACCTGGAAGGTTCCACGTGGGCAGTGC AGAGTCCATGCTACATGTTCGACCTGGAGGCTACACCCCTCAGAGAGCACTGATTAACCCCTTCGCCCCC TCAAGAATGCCCATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCATACTTTTCCTGTAGGACCATCTG GAGAGGCCATCCAGATCCATCATCAAACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGCGGGA CCCCACCCACTCCTCTGCAGAACTGTTGGAGCTGGCATATCATGAAGCCGCAGGAAGGCACAGCACTTCC CGACAGCCTGGTGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCA ACAGCGGCACAGGTATGAATCCTAGGAACCAGAATAAGGACTCTCTAGAGGATGGTGTTTCTACCTCTCC AGACCCAATGCCAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTACTACTCCTGCATGCCTT CCCCTCACCACTGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACAGAGGGCTGCTATGATC TCCTCCCAGAAGCAGACATGGACAGGAGGGACGAGGAGGGCGTTCAAATGACAGCCCAGCAAGTGTTTGA AGAGTTCATCTGCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAAGACCCAGAAGCCCAGC ACTACAGTCCCACCCCCGCTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTGTCCCGAAACCGCCCTG AAGAGGAGGGCCAGTATTGGCTGAGTATGGGCAGAACCTTCCATAAGGTGACACTTAAGGACAAGATGAT CACAGTGACTCGTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTACACCTACAGCCTCTGC CCTTCCCACTCTGACTCAGAGTTTGTCTCCTGTTGGGTGGAATTCTGCCATGAACGGCTAGAGGAATACA AGTGGAATTACTTAGATCAGTACATTTGTTCTGCCGGCTCCGAAGATTTCAGCTTAATCGAGTCTCTGAA GTTCTGGAGAACCCGATTTCTACTACTGCCAGCCTGTGTCACTGCCACCAAGCGCATCACAGAAGGGGAA GTGCACTGTGACATCTACGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAGCTTCTAGATGGCTTTA TTCGCTTTGTAGAGGGCTTGAACCGCATCCGCAGGCGCCACCGGTCAGACCGCATGATTCGGAAAGGGAC TGCCATGAAAGGCTTGCAGATGACCGGGCCCATCTCCGCACACTCTCTCGAGTCAACGGGCCCCCCAGTC GGGAAGAAAGGAACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAGGCTAGTCAGAAGAGCCTAGGAGAAC AGCAGACAAGTGTGCATGGGAAAAGCTCTACTCAGCCAGCTGAGAACAGCAGTGTTGCCATGACTCCCAC CTATGTGGACAGCCCACGCAAGGCATCTGTGGACCAAACAGCTCCTCTGGTGTTGGACAGCACCAGTTTA GGGGTCAGCACGTGCCAGTCCGTGGATAGAGGCAATAACCAGACCTTTGGGAACTCCCAGAACATAGAAC AGGCCTTTCCCTCTACAGGCTCTGGAGACGGCAGCTCTCAGCAGCACATAGCCAGCTCTCTGACATCGTC CTCTACCCTGGTGGAGATCCTGGAAGCCATGAAGCATCCCTCGACAGGAGTCCAGCTGCTGTCTGAGCAG AAGGGCCTTTCGCCGTGCTGCTTCATCAGTGCGGAGGTCGTACACTGGCTGATGAACAATGTGGAGGGGG TCCAGACGCAGGCGATGGGCATCGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATAGCGCATGCATC TGGAGAAGCATGGCGAACCTTCGTCTACGGCTTCTATTTCTACAAGATAGTCACGGACAGAGAGCCCGAA CGAGTGGCTATGCAGCAGCCCTCTGCCCCATGGCACACAGCAGGAGTGGATGACTTTGCCAGCTTCCAAC GCAAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTTGTGCACTCCGAGATTCCTGCCTTCCTCCTGCC CTGGCTACCTAGCCGGCCAGCCTCTTACGCAAGCAGGCACAGCTCCTTTAGCCGAAGTTTTGGAGGACGG AGCCAGGCAGCTGCACTGTTAGCTGCCACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTCGATGTGA ACAACCGAACGGACCGGCTGGAGTGGTGCAGCTGTTACTACCACGGAAACTTCTCTCTCAACGCAGCCTT TGAAATCAAGCTACACTGGATGGCAGTGACTGCCACAGTGCTGTTTGAGATGGTCCAAGGTTGGCATCGA AAAGCTACATCCTGCGGCTTCTTGTTAGTCCCGGTTTTGGAGGGTCCTTTTGCACTGCCCAGTTACCTGT ACGGTGACCCCTTGAGGGCCCAGCTCTTCATCCCGCTCAACCTCGGCTGCTTGCTCAAGGAGGGCAGCGA GCACCTGTTCGATAGCTTTGAACCAGAGACATATTGGGATCGAATGCACCTTTTCCAGGAAGCCATTGCA CACAGGTTTGGATTTGTGCAAGATAAATATTCTGCCTCCGCGTTTAACTTCCCTGCTGAGAATAAACCAC AGTATATCCACGTCACAGGAACAGTGTTTCTTCAGCTGCCGTACTCCAAACGCAAGTTTTCTGGGCAGCA GCGGCGGCGACGGAACTCTACCAGCTCCACCAACCAAAACATGTTCTGTGAGGAGCGGGTTGGCTACAAC TGGGCCTATAACACCATGCTGACCAAGACATGGCGCTCTAGTGCCACGGGGGATGAGAAGTTTGCCGATC GGCTGCTGAAGGACTTCACGGATTTCTGTATCAACCGTGACAACCGCCTGGTCACATTCTGGACAAACTG CCTGGAGAAGATGCACGCCAGCGCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206263 representing NM_001107229 Red=Cloning site Green=Tags(s) MWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEETRVVFRSTSAMVYIFIQMSCEMWD FDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSIDEFPEVNRASIQQDHKGRFYED FYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAGGFPQGDNSTSAQGNYLEAINLSFNVFDKHYIN RNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSVPRDSRL GDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKSATEKTKNGRDTSLGAPKESENTLPIQVDYDAYD AQVFRLPGPSRAQRLATCRSVREQENHNRKSASSCDVSSSPSLPSRALPTEEVRSQASDDSSLGKSTNIL MIPNPHLHQYEVSSSLGYTSTRDVLENMIEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAP SRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSRQRDPTHSSAELLELAYHEAAGRHSTS RQPGDSMSLNFSGTEELSVSLLSNSGTGMNPRNQNKDSLEDGVSTSPDPMPGFCCTVGVDWKSLTTPACL PLTTDYFPDRQGLQNDYTEGCYDLLPEADMDRRDEEGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPS TTVPPPLSSSPLYSRGLVSRNRPEEEGQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLC PSHSDSEFVSCWVEFCHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGE VHCDIYGDKPRADEDEWQLLDGFIRFVEGLNRIRRRHRSDRMIRKGTAMKGLQMTGPISAHSLESTGPPV GKKGTSALSALLEMEASQKSLGEQQTSVHGKSSTQPAENSSVAMTPTYVDSPRKASVDQTAPLVLDSTSL GVSTCQSVDRGNNQTFGNSQNIEQAFPSTGSGDGSSQQHIASSLTSSSTLVEILEAMKHPSTGVQLLSEQ KGLSPCCFISAEVVHWLMNNVEGVQTQAMGIDIMQKMLEEQLIAHASGEAWRTFVYGFYFYKIVTDREPE RVAMQQPSAPWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGR SQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTATVLFEMVQGWHR KATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNLGCLLKEGSEHLFDSFEPETYWDRMHLFQEAIA HRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYN WAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTNCLEKMHASAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107229
ORF Size	4368 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107229.1, NP_001100699.1
RefSeq Size	8076 bp
RefSeq ORF	4371 bp
Locus ID	305464
MW	164.7 kDa

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