Mtrr (NM_001039003) Rat Tagged ORF Clone
CAT#: RR206318
- TrueORF®
Mtrr (Myc-DDK-tagged ORF) - Rat 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr), (10 ug)
"NM_001039003" in other vectors (3)
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CNY 6,560.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MSR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206318 representing NM_001039003
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGGTTTTTGTTACTGTATGCTACACAGCGAGGGCAGGCAAAGGCCATAGCTGAAGAAATAAGTG AGCAGGCTCTGTCACACGGGTTTTCCGCAGATCTTCACTGCGTCAGTGAGTCAGAAAAGAGCTCTCTAAG TATTCCTGCTGTGTCCCCAGAATATTTGGAGGTCTATCTTCAGGAGTCTCTTGGCCAGGATGAAAACCAA GCATCTGTGCCACCATCAGTCGATCCAATTTTTCAAGTTCCAATTTCAAAAGCTGTTGAGCTGACTACAA ACGATGCCATAAAGACCACTCTGCTGTTGGAATTGGACATCTCAAAAGTAGAGTTTTCCCATCAACCTGG AGATTCCTTCAATGTGATCTGTCCAAACAGTGGTTCTGAAGTAGAAGACTTGCTGCAAAGGCTGCAGCTG GCTGACAAACAAGCACACCGGGTCATCCTGAAAATTAAGATGGACACCAAGAAGAAAGGAGCTTCTCTGC CCCAGCATGTGCCTGAGGGGAGTTCTCTCCAGTTCATCTTCACCTGGTGTCTTGAAATACGAGCAGTTCC TAAAAAGGCTTTTTTGAGGGCCCTTTCGGATTACACCAGCGATGCCACTGAAAAGCGGAGGCTTCAGGAG CTCTGCAGTAAGCAGGGAGCAGCTGATTACAACCGCTTCATCCGAGATGCCAGTGTCTGCCTGCTAGACC TCCTGCTTACCTTCCCATCCTGCCAGCCTCCGCTCAACCTCCTGCTGGAGCACCTTCCTAAACTCCAGCC TCGACCATACTCGTGCGCAAGCTCCAGCTTACTCCATCCAGACAAGCTTCACTTTGTGTTTAACATCGTA GAGCTTCCGTCGAACACCACTGCAGCCTCACTGCGGAAGGGAGTGTGCACAGGCTGGCTAGCCACATTGG TTGCTCCATTTCTTCAGCCAAACACAGAAGTTTTGACTGCAGACCATAGTGATGCTCTGGCTCCTGAGAT ACTTATCTCTCCTCGGGCAACAAATTCCTTCCACTTACCAGATGACCTGTCGGCCCCCATCATAATGGTG GGTCCAGGAACTGGTGTAGCCCCCTTTGTTGGCTTCCTGCAGCACAGAGAAAAACTCCAAGAACAACACC CAGACGGAAACTTTGGAGCAATGTGGCTGTTTTTTGGCTGCAGACATAAGGACAGAGACTATTTGTTCAG GGAGGAGCTCAGGCATTTCCTCAAGACTGGGGTCTTGACTCACCTGAAGGTCTCATTCTCAAGAGATGCT GCGCCTGAGGAAGAGGAGGAGGCCCCAGCAAAGTATGTGCAAGACAACCTCCAGCATCACAGCCAGCAGG TGGCCAGGACCCTCCTTCAGGAGAATGGCTACATTTACGTGTGTGGAGACGCCAAGAATATGGCTAAGGA TGTACACGACGCCCTTGTAGAAATCATAAGCAAAGAAGCTGGAGTTGACAAACTAGAAGCAATGAAGACA CTGGCAACCTTAAAGCAAGAAAAGCGGTATCTGCAGGATATCTGGTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206318 representing NM_001039003
Red=Cloning site Green=Tags(s) MRRFLLLYATQRGQAKAIAEEISEQALSHGFSADLHCVSESEKSSLSIPAVSPEYLEVYLQESLGQDENQ ASVPPSVDPIFQVPISKAVELTTNDAIKTTLLLELDISKVEFSHQPGDSFNVICPNSGSEVEDLLQRLQL ADKQAHRVILKIKMDTKKKGASLPQHVPEGSSLQFIFTWCLEIRAVPKKAFLRALSDYTSDATEKRRLQE LCSKQGAADYNRFIRDASVCLLDLLLTFPSCQPPLNLLLEHLPKLQPRPYSCASSSLLHPDKLHFVFNIV ELPSNTTAASLRKGVCTGWLATLVAPFLQPNTEVLTADHSDALAPEILISPRATNSFHLPDDLSAPIIMV GPGTGVAPFVGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFREELRHFLKTGVLTHLKVSFSRDA APEEEEEAPAKYVQDNLQHHSQQVARTLLQENGYIYVCGDAKNMAKDVHDALVEIISKEAGVDKLEAMKT LATLKQEKRYLQDIWS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001039003 |
ORF Size | 1518 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001039003.1, NP_001034092.1 |
RefSeq Size | 2387 bp |
RefSeq ORF | 1521 bp |
Locus ID | 290947 |
UniProt ID | Q498R1 |
MW | 56.5 kDa |
Gene Summary | Involved in the reductive regeneration of cob(I)alamin (vitamin B12) cofactor required for the maintenance of methionine synthase in a functional state. Necessary for utilization of methylgroups from the folate cycle, thereby affecting transgenerational epigenetic inheritance. Folate pathway donates methyl groups necessary for cellular methylation and affects different pathways such as DNA methylation, possibly explaining the transgenerational epigenetic inheritance effects.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206318 | Mtrr (untagged ORF) - Rat 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr), (10 ug) |
CNY 6,560.00 |
|
RR206318L3 | Lenti ORF clone of Mtrr (Myc-DDK-tagged ORF) - Rat 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr), (10 ug) |
CNY 8,460.00 |
|
RR206318L4 | Lenti ORF clone of Mtrr (mGFP-tagged ORF) - Rat 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr), (10 ug) |
CNY 9,220.00 |