Pgam5 (NM_001025272) Rat Tagged ORF Clone
CAT#: RR206386
- TrueORF®
Pgam5 (Myc-DDK-tagged ORF) - Rat phosphoglycerate mutase family member 5 (Pgam5), (10 ug)
"NM_001025272" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1312028 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206386 representing NM_001025272
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCCGGCAGGCTCTTCAGCTGGCGGCCTGTGGTCTGGCGGGTGGCTCGGCCGCTGTTCTCTTCT CGGCGGTAGCGGTAGGGAAGCCGAGAGCTGGCGGGGATGCAGACACGCGCACGACAGAGCCGCTGGCCTG GACCGGAGCGCGCCCCGGGCACGGCGTCTGGGACTCCAACTGGGACAGGCGAGAGCCACTGTCACTCATT AACCTGAAGAAAAGGAACGTGGAATTTGGAGAAGATGAACTGGCTTCCAGGCTGGACCACTATAAGGCAA AGGCTACAAGACACATATTCCTCATCCGGCATTCCCAGTACAATGTGGATGGCTCGATGGAGAAGGACCG CACCCTGACCCCATTAGGTCGGGAACAGGCTGAACTCACGGGGATCCGACTTGCAAGCCTGGGACTGAAG TTTAATAAAATCGTCCATTCCTCTATGACCCGTGCAGTAGAGACCACAGACATCATCAGTAAGCACCTGC CAGGTGTCTGCAGAGTCAGCACAGACTTGCTACGGGAAGGTGCCCCCATTGAACCGGATCCACCTGTGTC TCACTGGAAGCCAGAGGCTGTGCAGTATTATGAAGATGGAGCCCGGATTGAGGCTGCCTTCAGGAACTAC ATCCACCGAGCTGATGCCAAGCAGGAGGAGGACAGCTACGAGATCTTCATATGTCACGCCAATGTCATCC GTTATATTGTTTGTAGGGCACTGCAGTTTCCCCCAGAAGGTTGGCTCCGCCTGTCCCTCAACAATGGTAG TATCACCCACCTGGTGATTCGACCCAATGGTCGTGTTGCACTCAGGACCCTTGGGGACACAGGGTTCATG CCCCCAGACAAGATTACTCGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206386 representing NM_001025272
Red=Cloning site Green=Tags(s) MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDADTRTTEPLAWTGARPGHGVWDSNWDRREPLSLI NLKKRNVEFGEDELASRLDHYKAKATRHIFLIRHSQYNVDGSMEKDRTLTPLGREQAELTGIRLASLGLK FNKIVHSSMTRAVETTDIISKHLPGVCRVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNY IHRADAKQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFM PPDKITRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025272 |
ORF Size | 864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025272.1, NP_001020443.1 |
RefSeq Size | 2016 bp |
RefSeq ORF | 867 bp |
Locus ID | 288731 |
UniProt ID | Q562B5 |
MW | 32.1 kDa |
Gene Summary | Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206386 | Pgam5 (untagged ORF) - Rat phosphoglycerate mutase family member 5 (Pgam5), (10 ug) |
CNY 3,990.00 |
|
RR206386L3 | Lenti ORF clone of Pgam5 (Myc-DDK-tagged ORF) - Rat phosphoglycerate mutase family member 5 (Pgam5), (10 ug) |
CNY 6,080.00 |
|
RR206386L4 | Lenti ORF clone of Pgam5 (mGFP-tagged ORF) - Rat phosphoglycerate mutase family member 5 (Pgam5), (10 ug) |
CNY 6,650.00 |