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Gcs1 (NM_031749) Rat Tagged ORF Clone
CAT＃: RR206473
- TrueORF®
Gcs1 (Myc-DDK-tagged ORF) - Rat glucosidase 1 (Gcs1), (10 ug)
"NM_031749" in other vectors (3)
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CNY 10,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mogs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206473 representing NM_031749
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGGCGAGAGGCGACGGCGCGCTGCGGCCGCAGAGGGGGCGCGCCCGCTGGAGAGGGCGCGGG GTGCGGGCCGGCGGGATGGCAGAGCCGGCGGGGCGCGCGGCTCGGCGGGCGGCGCGGCCCTGGCCGTGGT GGTTTTGGCGCTGGCCTTCGGCCTGTCGGGGCGCTGGGTGCTGGCGTGGCTGGGTGTGCGCCGCGCGCTC ACACTGCACCCCGCTCCGTCAGCGCTACCGCCGGACTCCTCCAGTCCTGCCGTGGCCCCGGAATTTTTTT GGGGTACCTATCGCCCACACGTCTATTTCGGCATGAAGACTCGCAGCCCCAAACCACTGCTCACGGGACT GATGTGGGCGCAGCAAGGCGCCACCCCGGGGACCCCTCCTAAGCTCAGGCACACATGCGAACAAGGAGAC GGCGTGGGTCCCTATGGCTGGGAGTTCCATGATGGACTCTCCTTTGGTCGGCAACATATCTATGATGGGG CCTTAAGGCTCACCACTGAGTTCGTCAAGAGATCTGGGGGTCATCACGGAGGGGATTGGAGCTGGAGAGT TACCGTAGAGCCTCAGGCCTCAGGTACACCTTCCTTCCCTTTGGTCTCCCTGTTCTTCTACGTGGTAACA GATGGGCAAGAGGTCCTACTACCAGAGGTTGGAGCCAAAGGACAGTTGAAGTTCATCAGTGGGCACACCA GTGAACTTGGTGACTTCCGCCTCACACTTCTGCCACCAACCACTCCAGGAGACACTGTCCCTAAGCATGG CAGCTACAATGTCTTCTGGTCCTCCAACCCAGGGCTGCCTCTGCTCACAGACATGGTAAAGAGCCACCTA AACAGCTGGTTTCATCACCGGCCCCCAGGAGCCTCTCCGGAACGATACCTTGGCTTGCCTGGATCTCTTA AGTGGGAAGAGAGAGGCCCTAGTGGGCAAGGGCAGTTCTTGGTCCAGCAGGTGACACTGAAAGCCCCTTT CTCTGTGGAGTTTGTGTTTGAATCTGGCAGTGCCAGGACAGGAAGAGACCAAGCCTCTGAGCAGCTGGTA GGTGGCCAACTGACCCGGGCCCTGGAGAGCCATGCTGCAGCATTCAAGGAGCGTTTTGAGAGGACCTTCC AGCTAAAGGAGAAGGGCCTGAGCCCTGAGGAGCAGGCTCTGGGTCAGGTTGCCCTCAGTGGCCTACTTGG TGGGATTGGTTACTTCTATGGACAGGGTTTGGTGTTGCCAGACACTGGCATGGAAGGGTCTGAACAGAAA ATGGACCCTTCCCTATTTCCGCCTGTCCCCCTTTTCTCTGGGGTCCCTTCCCGGTCATTCTTCCCACGAG GCTTCCTCTGGGATGAAGGCTTTCACCAGCTAGTGGTCCAGCGATGGGATCCCCACCTCACCCGGGAGGC CCTAGGACACTGGCTGGGGCTGCTCAATGCTGACGGTTGGATTGGGCGAGAGCAGATTCTGGGGGATGAG GCCAGAGCCCGAGTGCCCCCGGAATTCCTAGTGCAACGTGCAGCCCATGCCAACCCTCCAACCCTGCTCC TGCCAGTCATACACATGCTAGAAGGCCGGGCCCCTGAGGACTTGGCCTTCCTGCGCAGGGCTTTCCCCCG CTTGCATGCCTGGTTCTCTTGGCTCCATCAAAGTCAGGCAGGGCCAGTGCCACTTTCCTACCGCTGGCGG GGCAGGGATCTAGCTTTGCCTACCCTACTTAACCCCAAGACACTGCCCTCGGGCCTGGATGACTACCCCA GGGCCTCACATCCTTCTGCAGCAGAGCGGCACCTGGACCTGCGGTGCTGGGTGACATTGGGTGCCCGGGT GCTGTCACAGCTTGCAGAAGAGCTTGGGGAGACTGAAGCTGCTGCAGAGTTGGGCCCATTGGCTGCCTCT CTAGAAGCAGCTGGAAGTCTCGATGAGCTGCACTGGGCTCCTGAGCTGGGTGTCTTTGCCGACTTTGGGA ACCACACAAAAGCAGTACAGCTCAAGTCTAGGCCCCCACAGGGGCTGGTTAGGGTGGTAGGCAGGCCTCC AGCTCGACTGCAGTATGTGGATGCCCTGGGCTATGTCTCTCTTTTTCCTTTGCTGCTACAACTGCTGGAG CCCAGCTCACCCCGCTTGGGACCCCTGCTGGACGTTCTAGCTGATAGCCGCCATCTCTGGAGCCCCTTTG GGTTGCGCTCCCTTTCAGCCTCCAGTCTCTTTTATAAACAGCGAAATACGGAACACGATCCACCTTACTG GCGAGGTGCGGTGTGGCTGAATATCAACTACCTGGCTTTGGGGGCACTACACCACTATGGGCGTGTGGAG GGTCCTCACAAGGTTCAGGCTGCCAAACTCTACCGTGAACTTCGGGCCAATGTAGTAAGCAACGTACGGC AGCAATACCAGGCTACAGGGTTTCTGTGGGAACAGTACAGTGACCAGGATGGGCGGGGCATGGGCTGCCG CCCCTTCCAAGGATGGACCAGTCTTGTTTTACTGATCATGGCTGAAGAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206473 representing NM_031749
Red=Cloning site Green=Tags(s) MARGERRRRAAAAEGARPLERARGAGRRDGRAGGARGSAGGAALAVVVLALAFGLSGRWVLAWLGVRRAL TLHPAPSALPPDSSSPAVAPEFFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPGTPPKLRHTCEQGD GVGPYGWEFHDGLSFGRQHIYDGALRLTTEFVKRSGGHHGGDWSWRVTVEPQASGTPSFPLVSLFFYVVT DGQEVLLPEVGAKGQLKFISGHTSELGDFRLTLLPPTTPGDTVPKHGSYNVFWSSNPGLPLLTDMVKSHL NSWFHHRPPGASPERYLGLPGSLKWEERGPSGQGQFLVQQVTLKAPFSVEFVFESGSARTGRDQASEQLV GGQLTRALESHAAAFKERFERTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQGLVLPDTGMEGSEQK MDPSLFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVVQRWDPHLTREALGHWLGLLNADGWIGREQILGDE ARARVPPEFLVQRAAHANPPTLLLPVIHMLEGRAPEDLAFLRRAFPRLHAWFSWLHQSQAGPVPLSYRWR GRDLALPTLLNPKTLPSGLDDYPRASHPSAAERHLDLRCWVTLGARVLSQLAEELGETEAAAELGPLAAS LEAAGSLDELHWAPELGVFADFGNHTKAVQLKSRPPQGLVRVVGRPPARLQYVDALGYVSLFPLLLQLLE PSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYLALGALHHYGRVE GPHKVQAAKLYRELRANVVSNVRQQYQATGFLWEQYSDQDGRGMGCRPFQGWTSLVLLIMAEEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031749 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031749.2, NP_113937.1 |
| RefSeq Size | 2702 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 78947 |
| UniProt ID | O88941 |
| MW | 91.9 kDa |
| Gene Summary | human homolog cleaves the distal alpha-1,2-linked glucose residue from Glc(3)-Man(9)-GlcNAc(2); involved in the N-linked oligosaccharide processing pathway [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206473 | Gcs1 (untagged ORF) - Rat glucosidase 1 (Gcs1), (10 ug) |
CNY 10,640.00 |
|
| RR206473L3 | Lenti ORF clone of Gcs1 (Myc-DDK-tagged ORF) - Rat glucosidase 1 (Gcs1), (10 ug) |
CNY 12,540.00 |
|
| RR206473L4 | Lenti ORF clone of Gcs1 (mGFP-tagged ORF) - Rat glucosidase 1 (Gcs1), (10 ug) |
CNY 13,680.00 |
