Abcc9 (NM_013040) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sur2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206569 representing NM_013040 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTTTCCTTCTGTGGTAACAACATCTCCTCCTACAACATCTACCATGGTGTTCTCCAAAACCCCT GCTTTGTGGACGCACTCAACCTGGTCCCTCACGTCTTCCTGCTGTTTATCACCTTTCCGATCCTGTTCAT TGGATGGGGGAGCCAAAGCTCAAAAGTGCAAATTCATCACAACACATGGCTTCATTTTCCTGGACACAAC CTGAGATGGATTCTGACGTTTGCACTCCTGTTTGTGCATGTCTGCGAGATAGCAGAAGGCATTGTTTCAG ACTCGCAACGGGCGTCCAGGCATCTCCACCTTTTCATGCCGGCTGTGATGGGATTTGTCGCCACCACCAC ATCCATTGTGTATTACCACAACATTGAAACATCAAACTTCCCTAAATTACTTTTAGCTTTATTCCTGTAC TGGGTCATGGCCTTCATTACAAAGACAATAAAGTTGGTCAAATACTGGCAGTTGGGGTGGGGAATGTCAG ACCTGCGCTTCTGCATCACGGGAGTGATGGTCATCTTGAATGGACTGCTGATGGCTGTGGAGATCAATGT CATCCGGGTCCGAAGATATGTTTTCTTCATGAATCCTCAGAAAGTGAAGCCTCCAGAGGACCTCCAGGAC CTGGGCGTGAGGTTCCTCCAGCCGTTTGTGAATTTGCTGTCCAAAGCTACTTACTGGTGGATGAACACGC TTATCATATCAGCTCACAGGAAACCTATTGATCTGAAGGCGATCGGAAAGTTGCCGATAGCAATGAGGGC GGTGACGAATTATGTTTGCTTGAAGGAGGCCTACGAAGAGCAGAAGAAAAAGGCTGCGGATCATCCGAAT CGGACTCCCTCCATATGGCTGGCCATGTACAGGGCTTTTGGGAGACCGATCCTGCTGAGCAGCACGTTCC GCTACTTGGCTGACTTGCTGGGCTTTGCCGGCCCTCTTTGTATTTCCGGAATCGTCCAGCGTGTGAATGA ACCGAAGAATAACACGACGAGATTTTCAGAGACACTCTCATCAAAGGAGTTTCTGGAAAATGCCCATGTG CTGGCTGTCCTGCTCTTCCTGGCCCTCATCCTGCAAAGAACATTTTTGCAGGCTTCGTACTATGTGACCA TAGAGACCGGCATCAACCTGCGTGGGGCTCTGCTGGCTATGATCTACAACAAAATCCTTCGACTGTCTAC TTCTAACCTATCCATGGGCGAGATGACCCTGGGACAGATCAACAACTTGGTTGCCATAGAAACCAATCAG CTCATGTGGTTCTTGTTCCTGTGTCCCAATCTGTGGGCCATGCCCGTTCAGATCATAATGGGGGTGATCC TGCTCTATAATCTGCTTGGGTCAAGCGCACTGGTAGGCGCGGCGGTCATCGTGCTCCTCGCACCGATTCA GTACTTCATCGCCACGAAGCTGGCGGAGGCTCAGAAGAGCACTCTGGATTATTCCACCGAGAGGCTGAAG AAGACAAACGAGATACTGAAAGGCATCAAGCTCCTTAAGCTGTATGCCTGGGAGCACATTTTCTGCAAGA GCGTGGAAGAGACTAGAATGAAGGAGCTCTCCAGCCTCAAAACCTTCGCGCTCTACACGTCGCTTTCCAT CTTCATGAACGCAGCCATTCCCATCGCAGCCGTTCTTGCAACATTTGTGACCCATGCCTATGCCAGTGGC AACAACCTGAAACCCGCAGAGGCCTTTGCCTCTCTGTCTCTCTTCCACATCCTCGTCACACCGCTCTTCC TGCTGTCCACGGTGGTCAGATTCGCAGTGAAAGCCATCATCAGCGTTCAGAAGCTGAATGAGTTTCTCTT GAGCGATGAGATTGGCGAGGACAGCTGGAGGACTGGGGAGGGGACGCTGCCTTTTGAGTCCTGTAAGAAG CACACCGGAGTGCAATCAAAACCGATAAACAGGAAGCAGCCTGGAAGGTACCACCTGGACAACTACGAGC AGGCGCGGCGTCTTCGGCCTGCTGAGACTGAAGATGTTGCCATAAAGGTGACGAACGGATACTTCTCATG GGGCAGTGGTTTAGCCACATTATCCAATATTGACATTCGAATTCCAACAGGTCAGCTAACCATGATTGTG GGTCAGGTGGGTTGTGGCAAATCGTCTCTCCTCCTTGCCATCCTTGGTGAGATGCAGACCCTGGAAGGAA AAGTTTACTGGAACAATGTAAATGAATCTGAGCCTTCTTTTGAAGCAACCCGAAGCAGAAGCAGGTACTC TGTGGCTTATGCTGCCCAGAAGCCTTGGCTCCTCAATGCTACGGTCGAGGAAAACATCACTTTTGGAAGT TCTTTCAACAGACAGAGGTACAAGGCTGTCACCGATGCCTGCTCTCTGCAGCCAGACATTGATTTGTTAC CCTTTGGAGACCAAACTGAAATTGGAGAGAGGGGTATCAACCTGAGTGGGGGTCAGAGGCAGAGAATCTG CGTGGCCCGGGCACTCTACCAGAACACCAATATTGTCTTCTTGGACGATCCATTCTCCGCTCTGGACATC CACCTGAGCGACCACTTGATGCAGGAAGGGATCCTGAAGTTTCTCCAGGACGACAAGAGGACGGTCGTCC TTGTGACTCACAAACTACAGTACCTGACGCACGCGGACTGGATCATAGCCATGAAGGATGGGAGTGTGTT AAGGGAAGGGACTTTGAAAGACATCCAGACCAAAGACGTGGAGCTCTATGAACACTGGAAAACCCTCATG AATCGGCAAGATCAGGAATTAGAAAAGGACATGGAAGCCGACCAAACAACGCTGGAGAGGAAGACTCTCC GAAGAGCTATGTACTCAAGGGAGGCCAAAGCACAGATGGAGGATGAAGATGAAGAGGAGGAGGAGGAGGA AGATGAGGACGACAACATGTCAACTGTAATGAGGCTCAGGACGAAGATGCCCTGGAAGACCTGTTGGTGG TACCTCACTTCAGGAGGGTTTTTCCTGCTCTTCCTCATGATCTTCTCTAAGCTTTTGAAGCACTCTGTGA TCGTGGCCATCGACTACTGGCTAGCTACGTGGACCTCCGAGTACAGTATAAACGACCCAGGGAAAGCTGA CCAGACCTTCTATGTGGCTGGGTTCAGCATCCTCTGTGGAGCGGGCATTTTCCTTTGCCTCGTCACCTCC CTCACTGTAGAATGGATGGGTCTCACCGCCGCCAAGAACCTCCACCACAATCTCCTCAATAAGATAATTC TGGGCCCAATAAGGTTCTTTGATACCACGCCCCTGGGACTGATCCTCAATCGGTTTTCTGCTGATACCAA CATCATCGACCAACATATCCCTCCGACCTTGGAGTCGCTGACCCGCTCTACCCTGCTCTGCCTGTCGGCT ATTGGGATGATCTCCTATGCTACACCCGTGTTTCTCATCGCTCTTGCGCCCCTGGGCGTCGCCTTTTATT TCATCCAGAAATACTTCCGGGTTGCCTCTAAGGATCTCCAGGAACTCGATGACAGCACCCAGCTCCCCCT GCTTTGTCACTTCTCAGAAACAGCTGAAGGGCTTACCACTATCCGGGCCTTCAGGCATGAAACCAGATTC AAGCAACGCATGCTGGAGCTGACAGACACAAACAACATTGCCTACTTATTTCTCTCCGCAGCCAACAGAT GGCTGGAGGTCAGGACGGACTACCTGGGAGCTTGCATTGTTCTGACGGCCTCCATTGCATCCATCAGTGG CTCTTCCAACTCTGGACTAGTGGGCTTGGGCCTTCTGTATGCCCTCACGATAACCAATTACCTGAATTGG GTTGTAAGGAACTTGGCCGACCTCGAAGTCCAGATGGGCGCAGTGAAGAAAGTGAACAGTTTCTTAACTA TGGAGTCTGAGAACTATGAAGGCACCATGGATCCTTCTCAAGTCCCAGAGCATTGGCCACAGGAAGGTGA GATCAAGATTCACGATCTATGCGTCAGATATGAAAATAACCTGAAGCCCGTTCTGAAACATGTCAAGGCT TACATCAAGCCTGGGCAGAAGGTGGGCATCTGTGGTCGCACCGGTAGTGGGAAGTCCTCTCTATCTCTGG CTTTCTTCAGAATGGTCGACATATTTGATGGAAAGATAGTCATTGATGGAATAGACATTTCCAAGCTGCC CTTGCACACGCTCCGCTCTAGACTGTCCATCATTCTCCAGGACCCAATCCTGTTCAGCGGCTCTATCAGA TTTAACTTGGATCCTGAATGCAAGTGCACAGACGACAGGCTCTGGGAGGCTCTGGAAATTGCTCAGTTGA AGAATATGGTCAAATCTCTGCCAGGAGGCCTAGACGCCACTGTCACCGAAGGTGGTGAGAACTTCAGCGT TGGACAGAGACAGCTGTTCTGCCTGGCCAGGGCCTTTGTTCGAAAGAGCAGCATACTCATTATGGATGAG GCCACGGCCTCCATCGACATGGCCACGGAAAACATTTTGCAGAAAGTAGTCATGACAGCCTTTGCGGATC GCACGGTTGTAACCATAGCTCATCGGGTTCACACCATTCTGACTGCTGACCTGGTCATTGTGATGAAGAG AGGAAACATTTTGGAATACGACACCCCGGAAAGCCTCTTGGCCCAGGAGGATGGAGTGTTCGCCTCGTTC GTTCGTGCGGACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206569 representing NM_013040 Red=Cloning site Green=Tags(s) MSLSFCGNNISSYNIYHGVLQNPCFVDALNLVPHVFLLFITFPILFIGWGSQSSKVQIHHNTWLHFPGHN LRWILTFALLFVHVCEIAEGIVSDSQRASRHLHLFMPAVMGFVATTTSIVYYHNIETSNFPKLLLALFLY WVMAFITKTIKLVKYWQLGWGMSDLRFCITGVMVILNGLLMAVEINVIRVRRYVFFMNPQKVKPPEDLQD LGVRFLQPFVNLLSKATYWWMNTLIISAHRKPIDLKAIGKLPIAMRAVTNYVCLKEAYEEQKKKAADHPN RTPSIWLAMYRAFGRPILLSSTFRYLADLLGFAGPLCISGIVQRVNEPKNNTTRFSETLSSKEFLENAHV LAVLLFLALILQRTFLQASYYVTIETGINLRGALLAMIYNKILRLSTSNLSMGEMTLGQINNLVAIETNQ LMWFLFLCPNLWAMPVQIIMGVILLYNLLGSSALVGAAVIVLLAPIQYFIATKLAEAQKSTLDYSTERLK KTNEILKGIKLLKLYAWEHIFCKSVEETRMKELSSLKTFALYTSLSIFMNAAIPIAAVLATFVTHAYASG NNLKPAEAFASLSLFHILVTPLFLLSTVVRFAVKAIISVQKLNEFLLSDEIGEDSWRTGEGTLPFESCKK HTGVQSKPINRKQPGRYHLDNYEQARRLRPAETEDVAIKVTNGYFSWGSGLATLSNIDIRIPTGQLTMIV GQVGCGKSSLLLAILGEMQTLEGKVYWNNVNESEPSFEATRSRSRYSVAYAAQKPWLLNATVEENITFGS SFNRQRYKAVTDACSLQPDIDLLPFGDQTEIGERGINLSGGQRQRICVARALYQNTNIVFLDDPFSALDI HLSDHLMQEGILKFLQDDKRTVVLVTHKLQYLTHADWIIAMKDGSVLREGTLKDIQTKDVELYEHWKTLM NRQDQELEKDMEADQTTLERKTLRRAMYSREAKAQMEDEDEEEEEEEDEDDNMSTVMRLRTKMPWKTCWW YLTSGGFFLLFLMIFSKLLKHSVIVAIDYWLATWTSEYSINDPGKADQTFYVAGFSILCGAGIFLCLVTS LTVEWMGLTAAKNLHHNLLNKIILGPIRFFDTTPLGLILNRFSADTNIIDQHIPPTLESLTRSTLLCLSA IGMISYATPVFLIALAPLGVAFYFIQKYFRVASKDLQELDDSTQLPLLCHFSETAEGLTTIRAFRHETRF KQRMLELTDTNNIAYLFLSAANRWLEVRTDYLGACIVLTASIASISGSSNSGLVGLGLLYALTITNYLNW VVRNLADLEVQMGAVKKVNSFLTMESENYEGTMDPSQVPEHWPQEGEIKIHDLCVRYENNLKPVLKHVKA YIKPGQKVGICGRTGSGKSSLSLAFFRMVDIFDGKIVIDGIDISKLPLHTLRSRLSIILQDPILFSGSIR FNLDPECKCTDDRLWEALEIAQLKNMVKSLPGGLDATVTEGGENFSVGQRQLFCLARAFVRKSSILIMDE ATASIDMATENILQKVVMTAFADRTVVTIAHRVHTILTADLVIVMKRGNILEYDTPESLLAQEDGVFASF VRADM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013040
ORF Size	4635 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013040.2, NP_037172.2
RefSeq Size	6628 bp
RefSeq ORF	4638 bp
Locus ID	25560
UniProt ID	Q63563
MW	174.3 kDa
Gene Summary	ATP-sensitive potassium channel subunit that confers ATP sensitivity and acts as receptor for sulfonylurea; may play a role in mesangial cell contractility in response to calcium ion transients [RGD, Feb 2006]

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