Acacb (NM_053922) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Acc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206624 representing NM_053922 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTTGCTTCTCTTTCTGACTTACCTGGTTTTCTCCTGCCTGACCATTTCCTGGTTAAAAATCTGGG GGAAGATGACAGACTCGAGGCCGCTCAGCAACAGTAAGGTGGATGCAAGCCTCCTTCCGAGCAAGGAGGA GTCTTTTGCCTCGGACCAGTCAGAGGAGCATGGCGACTGCAGCTGTCCGTTGACAACTCCTGACCAGGAG GAGCTGGCCTCCCACGGAGGTCCTGTAGATGCCAGTCAGCAGAGGAACTCTGTACCAACCTCACACCAGA AGCCTCCGAGGAACCCACTATCTTCCAATGACACCTGTTCCTCCCCAGAACTCCAAACCAACGGGGTAGC AGCACCTGGCTCAGAGGTTCCAGAAGCCAACGGGTTGCCTTTCCCAGCCAGGCCTCAGACCCAGAGAACG GGATCCCCCACTAGGGAGGACAAGAAGCAGGCACCCATCAAGAGGCAGCTGATGACCAGCTTTATCCTGG GCTCCCTCGATGACAACTCCTCTGACGAGGACCCTAGTTCTAACTCCTTTCAGACCTCCTCTCGGAAGGG CAGCAGGGATAGCCTGGGCACCTGTTCCCAGGAGGCTGCATTGAACACAGCTGATCCTGAGTCTCACACA CCTACTATGAGGCCCAGCATGTCTGGACTCCATCTGGTGAAGAGAGGCCGTGAACACAAGAAACTGGACC TGCACAGAGATTTCACTGTAGCTTCCCCAGCCGAATTTGTCACCCGCTTTGGAGGCAACAGGGTTATCGA GACGGTGCTCATCGCCAATAATGGTATCGCTGCGGTCAAGTGGATGCGCTCCATCCGCCGCTGGGCCTAT GAGATGTTCCGTAATGAACGCGCTATCCGGTTTGTGGTTATGGTGACACCCGAGGATCTTAAGGCCAACG CAGAGTACTACAAGATGGCGGACCCAGTACTTCCGGTCCCAGGAGGACCCAATAATAACAACTACGCCAA CGTTGAGCTGATCATAGACATTGCCAAGAGAATCCCTGTGCAGGCCGTGTGGGCTGGCTGGGGCCACGCT TCGGAAAACCCCAAACTTCCAGAGCTACTGTGCAAGCACGGGATTGCTTTTCTAGGTCCCCGAGTGAGGC CAATGTTGGGCCTGGGAGACAGGCTCTCCTCCACCATTGTAGCCCAGACATTGCAGATCCCAACTCTACC CTGGAGCGGAAGCGGTCTCACAGTGGAGTGGACGGAGGACAGCCAGCATCAGGGCAAATGCATCAGCGTC ACGGAAGACGTTTATGAACAAGGCTGTGTGAGAGATGTGGACGAAGGCTTGCAGGCAGCAGAAAAAGTAG GATTTCCTCTGATGATCAAAGCCTCTGAAGGTGGAGGAGGGAAAGGAATCCGGCAGGCTGAGAGTGCAGA GGACTTCCCATGCTTTTTCAGACAGGTGCAGAGTGAGATCCCGGGCTCGCCCATCTTTCTCATGAAGCTG GCCCAGAATGCCCGGCACTTGGAGGTCCAGGTCTTGGCAGATCAGTATGGGAACGCAGTGTCCCTGTTTG GACGAGACTGCTCCATCCAGAGGCGGCACCAGAAGATCATTGAGGAGGCTCCGGCCAACATCGCTGCTCC GGCTGTGTTTGAGTTCATGGAACAGTGTGCCGTCCTCCTGGCCAAGACTGTGGTTTATGTGAGCGCGGGA ACCGTGGGGTACCTATACAGCCAGGATGGCAGCTTTCACTTCTTGGAGCTGAACCCACGCCTGCAGGTGG AACATCCCTGCACTGAAATGATTGCAGATGTCAACCTGCCCGCTGCACAGTTACAGATCGCCATGGGCGT GCCCCTGCACCGGCTGAAGGACATACGGCTTCTGTACGGAGAGTCCCCCTGGGGAGTGACCCCCGTTTCT TTTGAGACCCCTTTGAGCCCTCCCATTGCCCGAGGCCATGTCATTGCAGCCAGGATCACCAGCGAAAACC CAGACGAGGCCTTTAAGCCAAGCTCAGGGACAGTACAGGAGCTGAACTTCCGCAGCAACAAGAACGTGTG GGGTTACTTCAGCGTGGCCGCTGCTGGAGGCTTGCACGAGTTTCCGATTTCCCAGTTTGGGCACTGCTTC TCCTGGGGCGAGAACCAGGAAGAGGCTATTTCGAACATGGTGGTGGCTTTGAAAGAACTGTCTATCCGGG GTGACTTCCGGACCACCGTGGAATATCTCGTCAACCTTCTGGAGACGGAGAGCTTACAGAACAATGATAT CGACACGGGGTGGCTGGACCACCTCATCGCTCAGCGGGTGCAGGCAGAGAAGCCGGACATCATGCTCGGG GTGGTGTTTGGGGCCTTGAACGTGGCAGACGCAATGTTCAGAACCTGTATTACGGAATTCCTGCATTCCT TGGAAAGGGGTCAGGTCCTCCCGGCTGATTCTCTGCTGAACATCGTGGACGTTGAATTGATTTACGGAGG CATCAAATATGTTCTCAAGGTGGCCCGGCAGTCCCTGACCATGTTTGTCCTCATCATGAATGGTTGCCAC ATCGAGATCGATGCCCACCGGCCGAACGATGGGGGCCTGCTCCTGTCCTACAATGGTAGCAGTTACACTA CATACATGAAGGAAGAGGTGGACAGTTACCGGATCACTATCGGCAATAAGACATGCGTGTTTGAAAAGGA AAACGACCCCACCGTCCTGAGATCCCCCTCGGCTGGGAAGCTGATGCAGTACACGGTGGAGGATGGCCAG CACGTGGAAGTCGGGAGCAGCTATGCTGAGATGGAGGTGATGAAGATGATCATGACCCTGAACGTGCAAG AGAGCGGCCGGGTGAACTACATCAAGCGACCAGGGGCGGTATTGGAGGCTGGCTGCGTGGTGGCAAAGCT AGAACTCGATGACCCTTCAAAAGTGCACGCGGCACAGCCGTTCACAGGGGAGCTCCCCGCCCAGCAGACT CTGCCCATCCTCGGGGAGAGGCTGCATCAGGTGTTCCACAGCGTCTTGGAAAATCTGACCAATGTCATGA ATGGCTACTGCCTGCCCGAGCCCTTCTTCAGCATGAAGCTGAAGGACTGGGTGGAGAAGCCCATGATGAC TCTCCGGCATCCCTCCCTACCTCTGCTGGAGCTGCAGGAGATCATGACCAGCGTGGCAGACCGCATCCCG GTTCCGGTGGAGAAGGCAGTCCGCAGGGTGTTTGCGCAGGACGCCAGCAACATCACTTCGGTGCTCTGCC AGTTCCCCAGCCAGCAGATAGCCACCATCCTGGACTGCCACGCCGCCACCCTGCAGCGTAAGGTGGACCG AGAGGCCTTCTTCATGAACACACAGAGCATCGTGCAGCTGATCCAGAGATACCGCAGTGGGACCCGTGGC ATCATGAAGGCTGTGGTGCTAGACCTCCTGAGGAGATATCTGAACGTGGAGCATCATTTCCAGCAAGCCC ACTATGACAAGTGTGTGATCAACCTGAGGGAGCAGTTCAAGGCGGACATGACTCGGGTGCTGGACTGCAT CTTCTCACACTCACAAGTGGCCAAGAAGAACCAGCTGGTGACCATGTTGATAGATGAGCTGTGTGGCCCA GACCCCACCCTGTCAGAAGAGCTGACCTCCATCCTCAAGGAACTCACGCAGTTGAGCAGGAGTGAGCACT GCAAGGTGGCCCTCAGAGCCAGGCAGGTCCTGATTGCCTCTCACCTCCCCTCCTACGAGCTGCGGCACAA CCAGGTGGAGTCATCTTCCTGTCAGCCATTGACATGTAATGGCCACCAGTTCTGCCCGGAAAACCTCAAG AAACTAATACTTTCGGAAACGACCATATTCGATGTCCTGCCCACTTTCTTCTATCACGCTAACAAGGTCG TCTGTATGGCGTCCCTGGAGGTTTATGTGAGGAGAGGTTACATCGCCTACGAGTTAAACAGCCTACAGCA CCGGGAGCTCCCTGACGGCACCTGCGTGGTGGAGTTCCAGTTCATGCTGCCGTCTTCCCACCCCAACCGG ATGGCCATGCCCATCAATGTCTCTGACCCTGACCTGCTGAGACACAGTAAGGAACTCTTCATGGACAGTG GCTTCTCCCCACTGTGTCACCAGCGGATGGGGGCCATGGTGGCCTTCAGGAGATTTGAGGAGTTCACCAG GAACTTCGATGAAGTCATCTCCTGCTTTGCCAACGTGCCTACAGACACTCCTCTCTTCAGTAAGGCGTGC ACTTCCCTCTACTCAGAGGAGGACAGCAAGAGCCTTCAAGAGGAGCCCATCCACATCCTGAATGTGGCCA TCCAGTGCGCCGACCACATGGAGGACGAGAGACTGGTGCCGGTTTTCCGTGCCTTTGTACAGTCCAAGAA ACACATCCTTGTGGATTACGGACTGCGAAGAATCACATTCCTTATCGCCCAAGAGAAGGAATTTCCCAAG TTCTTCACGTTCAGAGCGAGAGATGAGTTTGCAGAAGACCGGATTTACCGCCACTTGGAGCCGGGCCTGG CCTTCCAGCTGGAGCTGAGCCGGATGCGCAACTTTGACTTGACGGCCGTGCCCTGTGCCAACCATAAGAT GCATCTTTACCTGGGAGCCGCCAAGGTGAAGGAAGGGCTGGAGGTGACTGACCACAGGTTCTTCATCCGA GCCATCATAAGGCACTCAGACCTGATCACCAAGGAAGCCTCCTTCGAGTACCTGCAGAATGAAGGGGAGC GGCTGCTGCTGGAAGCCATGGACGAGCTGGAGGTGGCGTTCAACAACACCAGCGTGCGCACTGACTGCAA CCACATCTTCCTCAACTTCGTGGCCCACGTCATCATGGACCCACTCAAGATCGAGGAGTCGGTGCGTGCC ATGGTCATGCGTTACGGCAGTCGGCTGTGGAAGCTCCGTGTGCTGCAGGCACAAGTTAAGATCAACATCC GTCAGACGACCTCGGACTGCGCCGTCCCCATTCGCCTCTTCATCACCAACGAGTCCGGCTACTACCTGGA CATCAGCCTCTACAAAGAAGTGACCGACTCCAGATCCGGAAACATCATGTTTCATTCCTTCGGCAACAAA CAAGGGAGCCTGCACGGGATGCTGATCAACACGCCCTACGTCACCAAGGATCTGCTCCAAGCCAAGCGAT TCCAGGCGCAGTCCCTGGGGACCACCTATGTGTACGACTTCCCAGAGATGTTCAGGCAGGCTCTCTTTAA ATTGTGGGGCTCCCCAGAGAAGTACGGGCCCGATATCCTGACATACACAGAGCTGGTGTTGGACTCCCAG GGCCAGCTGGTGGAGATGAACCGGCTTCCTGGTTGTAACGAGGTGGGCATGGTGGTTTTCAAAATGAGGT TCAAGACCCCGGAGTATCCAGAAGGCCGGGACACTATCGTCATCGGCAACGACATTACCTTCCAAATCGG CTCTTTCGGCATAGGAGAGGACTTCCTGTATCTACGGGCATCGGAGATGGCCCGGACAGAGGGCATCCCC CAAATCTATCTGGCAGCCAACAGCGGCGCCGTATTGGGCCTGTCCGAGGAGATCAAGCAGATATTCCAAG TGGCATGGGTGGACCCTGAGGATCCCTACAAAGGATTTAGATACCTGTACCTGTACCTGACGCCCCAAGA CTACACCCAGATCAGCTCCCAGAACTCCGTGCACTGCAAACACATCGAGGACGAAGGCGAGTCAGGTATT ATCGTTGATGTCATCGGGAAGGACAGCAGCCTGGGTGTGGAGAACCTGCGGGGCTCGGGCATGATTGCAG GAGAGGCTTCTCTGGCTTACGAAAAAAATGTCACCATCAGCATGGTCGACTGCCGCGCCATCGGAATCGG GGCTTACCTGGTGAGGCTGGGCCAGCGGGTGATCCAGGTGGAAAACTCCCACATCATCCTCACGGGAGCC GGTGCTCTCAACAAGGTTCTGGGAAGAGAGGTCTACACATCCAACAACCAACTGGGCGGTGTGCAGATCA TGCACACCAACGGGGTCTCCCACGTCACGGTGCCAGATGACTTCGAGGGGGTCTGCACCATTCTGGAATG GCTGTCATATATACCAAAGGACAATCAAAGCCCAGTCCCCATCATCACTCCTTCTGACCCCATCGACAGG GAAATTGAATTCACCCCAACCAAAGCTCCCTATGACCCCAGGTGGCTGCTGGCAGGGAGGCCTCACCCAA CTCTGAAGGGGACCTGGCAGAGTGGATTCTTCGACCATGGCAGTTTCAAGGAAATCATGGCACCCTGGGA CCAGACTGTGGTGACTGGACGAGCAAGGCTGGGGGGCATCCCTGTAGGGGTGATTGCCGTGGAGACTCGG TCTGTGGAGGTGGCTGTCCCTGCTCACCCAGCCAACTTGGATTCTGAGGCCAAGATCATCCAGCAGGCAG GCCAGGTGTGGTTCCCGGACTCTGCCTTCAAGACGGCTCAGGTCATCAGGGACTTCAACCAGGAGCATCT GCTTCTCATGATCTTTGCTAACTGGAGAGGCTTCTCGGGCGGCATGAAAGACATGTCCGAGCAGATGCTG AAGTTTGGCGCCTACATCGTGGACAGTCTCCGTCTGTCCAAGCAGCCAGTCCTCATCTATATCCCTCCCG GTGCCGAACTCCGAGGGGGCTCCTGGGTTGTCCTCGACTCCAGCATCAACCCCCTGTGCATAGAGATGTA CGCAGACAAAGAGAGCAGGGGGGGTGTTCTGGAGCCCGAGGGCACTGTGGAGATTAAGTTCCGGAAGAAA GATTTGGTGAAGACCATAAGGAGGATTGACCCAGTGTGCAAGAAACTCCTGGAACCAGCTGGGGACACCC AGCTCCCTGACAAGGACCGGAAAGAGCTGGAGAGCCAGCTGAAGGCCCGGGAGGACCTGCTGCTCCCCAT CTACCACCAGGTGGCAGTGCAGTTCGCCGACCTGCATGACACGCCGGGCCACATGCTGAAGAAGGGAATC ATTTCTGATGTCCTGGAGTGGAAGACCACACGTACTTACTTCTACTGGAGGCTGCGCCGGCTGCTGCTGG AGGCACAGGTGAAGCAGGAGATTCTGCGAGCCAGCCCTGAGCTGAGCCATGAGCACACGCAGTCCATGCT GCGACGCTGGTTTGTGGAGACCGAGGGCGCCGTCAAGGCCTACCTGTGGGACAGCAACCAGGTGGTAGTC CAGTGGCTGGAACAGCACTGGTCAGCCAGGGACAACCTGCGTTCCACTATCCGAGAGAACCTCAATTATC TGAAGCGGGACTCTGTCCTCAAGACCATCCAAAGCCTAGTTCAAGAACACCCAGAGGCGACCATGGGACT GTGTGGATACCTGAGCCAGCACCTCACGCCCGCTGAGCAGATGCAGGTGGTTCAGCTGCTGTCGACCACG GAGAGCCCAGCTTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206624 representing NM_053922 Red=Cloning site Green=Tags(s) MVLLLFLTYLVFSCLTISWLKIWGKMTDSRPLSNSKVDASLLPSKEESFASDQSEEHGDCSCPLTTPDQE ELASHGGPVDASQQRNSVPTSHQKPPRNPLSSNDTCSSPELQTNGVAAPGSEVPEANGLPFPARPQTQRT GSPTREDKKQAPIKRQLMTSFILGSLDDNSSDEDPSSNSFQTSSRKGSRDSLGTCSQEAALNTADPESHT PTMRPSMSGLHLVKRGREHKKLDLHRDFTVASPAEFVTRFGGNRVIETVLIANNGIAAVKWMRSIRRWAY EMFRNERAIRFVVMVTPEDLKANAEYYKMADPVLPVPGGPNNNNYANVELIIDIAKRIPVQAVWAGWGHA SENPKLPELLCKHGIAFLGPRVRPMLGLGDRLSSTIVAQTLQIPTLPWSGSGLTVEWTEDSQHQGKCISV TEDVYEQGCVRDVDEGLQAAEKVGFPLMIKASEGGGGKGIRQAESAEDFPCFFRQVQSEIPGSPIFLMKL AQNARHLEVQVLADQYGNAVSLFGRDCSIQRRHQKIIEEAPANIAAPAVFEFMEQCAVLLAKTVVYVSAG TVGYLYSQDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGVPLHRLKDIRLLYGESPWGVTPVS FETPLSPPIARGHVIAARITSENPDEAFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFPISQFGHCF SWGENQEEAISNMVVALKELSIRGDFRTTVEYLVNLLETESLQNNDIDTGWLDHLIAQRVQAEKPDIMLG VVFGALNVADAMFRTCITEFLHSLERGQVLPADSLLNIVDVELIYGGIKYVLKVARQSLTMFVLIMNGCH IEIDAHRPNDGGLLLSYNGSSYTTYMKEEVDSYRITIGNKTCVFEKENDPTVLRSPSAGKLMQYTVEDGQ HVEVGSSYAEMEVMKMIMTLNVQESGRVNYIKRPGAVLEAGCVVAKLELDDPSKVHAAQPFTGELPAQQT LPILGERLHQVFHSVLENLTNVMNGYCLPEPFFSMKLKDWVEKPMMTLRHPSLPLLELQEIMTSVADRIP VPVEKAVRRVFAQDASNITSVLCQFPSQQIATILDCHAATLQRKVDREAFFMNTQSIVQLIQRYRSGTRG IMKAVVLDLLRRYLNVEHHFQQAHYDKCVINLREQFKADMTRVLDCIFSHSQVAKKNQLVTMLIDELCGP DPTLSEELTSILKELTQLSRSEHCKVALRARQVLIASHLPSYELRHNQVESSSCQPLTCNGHQFCPENLK KLILSETTIFDVLPTFFYHANKVVCMASLEVYVRRGYIAYELNSLQHRELPDGTCVVEFQFMLPSSHPNR MAMPINVSDPDLLRHSKELFMDSGFSPLCHQRMGAMVAFRRFEEFTRNFDEVISCFANVPTDTPLFSKAC TSLYSEEDSKSLQEEPIHILNVAIQCADHMEDERLVPVFRAFVQSKKHILVDYGLRRITFLIAQEKEFPK FFTFRARDEFAEDRIYRHLEPGLAFQLELSRMRNFDLTAVPCANHKMHLYLGAAKVKEGLEVTDHRFFIR AIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFNNTSVRTDCNHIFLNFVAHVIMDPLKIEESVRA MVMRYGSRLWKLRVLQAQVKINIRQTTSDCAVPIRLFITNESGYYLDISLYKEVTDSRSGNIMFHSFGNK QGSLHGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGSPEKYGPDILTYTELVLDSQ GQLVEMNRLPGCNEVGMVVFKMRFKTPEYPEGRDTIVIGNDITFQIGSFGIGEDFLYLRASEMARTEGIP QIYLAANSGAVLGLSEEIKQIFQVAWVDPEDPYKGFRYLYLYLTPQDYTQISSQNSVHCKHIEDEGESGI IVDVIGKDSSLGVENLRGSGMIAGEASLAYEKNVTISMVDCRAIGIGAYLVRLGQRVIQVENSHIILTGA GALNKVLGREVYTSNNQLGGVQIMHTNGVSHVTVPDDFEGVCTILEWLSYIPKDNQSPVPIITPSDPIDR EIEFTPTKAPYDPRWLLAGRPHPTLKGTWQSGFFDHGSFKEIMAPWDQTVVTGRARLGGIPVGVIAVETR SVEVAVPAHPANLDSEAKIIQQAGQVWFPDSAFKTAQVIRDFNQEHLLLMIFANWRGFSGGMKDMSEQML KFGAYIVDSLRLSKQPVLIYIPPGAELRGGSWVVLDSSINPLCIEMYADKESRGGVLEPEGTVEIKFRKK DLVKTIRRIDPVCKKLLEPAGDTQLPDKDRKELESQLKAREDLLLPIYHQVAVQFADLHDTPGHMLKKGI ISDVLEWKTTRTYFYWRLRRLLLEAQVKQEILRASPELSHEHTQSMLRRWFVETEGAVKAYLWDSNQVVV QWLEQHWSARDNLRSTIRENLNYLKRDSVLKTIQSLVQEHPEATMGLCGYLSQHLTPAEQMQVVQLLSTT ESPASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053922
ORF Size	7368 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053922.1, NP_446374.1
RefSeq Size	7371 bp
RefSeq ORF	7371 bp
Locus ID	116719
MW	276.1 kDa
Gene Summary	catalyzes the carboxylation of acetyl-CoA to malonyl-CoA during fatty acid biosynthesis; involved in fatty acid oxidation and insulin secretion signaling [RGD, Feb 2006]

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