Dhfr (NM_130400) Rat Tagged ORF Clone
CAT#: RR206722
- TrueORF®
Dhfr (Myc-DDK-tagged ORF) - Rat dihydrofolate reductase (Dhfr), (10 ug)
"NM_130400" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Dhfr1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206722 representing NM_130400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCGTCCGCTGAACTGCATCGTCGCCGTGTCCCAAAATATGGGCATTGGCAAGAACGGAGACTTAC CCTGGCCTCTGCTCAGGAATGAGTTCAAGTACTTCCAGAGAATGACCACAACCTCTTCAGTAGAAGGTAA ACAGAATCTGGTGATTATGGGTAGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGAC AGAATTAATATAGTTCTCAGTAGAGAACTCAAGGAACCACCACAAGGAGCTCATTTTCTTGCCAAAAGTC TGGATGATGCCTTAAAACTTATTGAACAACCAGAGCTAGCAAGTAAAGTGGACATGGTCTGGGTAGTCGG AGGCAGCTCTGTCTACCAGGAAGCCATGAATCAGCCAGGCCACCTCAGACTCTTTGTGACAAGGATCATG CAGGAGTTTGAAAGTGACACATTCTTCCCAGAAATTGATTTGGAGAAATATAAACTTCTCCCAGAATACC CAGGCGTCCTCTCTGAGATCCAGGAGGAAAAAGGCATCAAGTACAAGTTTGAAGTCTATGAAAAGAAAGA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206722 representing NM_130400
Red=Cloning site Green=Tags(s) MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKD RINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIM QEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_130400 |
ORF Size | 561 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_130400.2, NP_569084.1 |
RefSeq Size | 760 bp |
RefSeq ORF | 564 bp |
Locus ID | 24312 |
UniProt ID | Q920D2 |
MW | 21.6 kDa |
Gene Summary | key component of purine and pyrmidine biosynthesis pathway; NADPH-dependent molecule reduces dihydrofolate to tetrahydrofolate [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206722 | Dhfr (untagged ORF) - Rat dihydrofolate reductase (Dhfr), (10 ug) |
CNY 3,990.00 |
|
RR206722L3 | Lenti ORF clone of Dhfr (Myc-DDK-tagged ORF) - Rat dihydrofolate reductase (Dhfr), (10 ug) |
CNY 6,080.00 |
|
RR206722L4 | Lenti ORF clone of Dhfr (mGFP-tagged ORF) - Rat dihydrofolate reductase (Dhfr), (10 ug) |
CNY 6,650.00 |