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Sik1 (NM_021693) Rat Tagged ORF Clone
CAT＃: RR206846
- TrueORF®
Sik1 (Myc-DDK-tagged ORF) - Rat SNF1-like kinase (Snf1lk), (10 ug)
"NM_021693" in other vectors (3)
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CNY 9,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sik; Snf1lk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206846 representing NM_021693
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATCATGTCGGAGTTCAGCGCGGTCCCCACAGGCACCGGCCAGGGCCAGCAGAAGCCCCTCCGGG TGGGCTTTTACGACGTGGAACGAACCCTGGGCAAAGGCAATTTTGCAGTGGTTAAGCTGGCGCGGCACCG AGTCACCAAAACGCAGGTTGCAATTAAAATAATTGACAAGACACGGTTAGATTCTAGTAATCTGGAGAAG ATCTACCGGGAGGTCCAGCTCATGAAACTCCTGAACCACCCAAATATTATCAAGCTTTACCAGGTTATGG AGACAAAAGACATGCTGTACATTGTCACGGAGTTTGCCAAAAATGGAGAAATGTTCGATTATCTGACTTC CAACGGGCACTTGAGTGAAAACGAGGCTAGGAAGAAATTCTGGCAGATCCTGTCAGCCGTGGAGTACTGC CACAACCATCACATCGTCCACCGGGACCTTAAGACAGAGAACCTTCTGCTGGACGGCAACATGGACATCA AGCTGGCAGATTTTGGATTTGGGAATTTCTACAAGCCAGGGGAGCCTCTGTCTACTTGGTGTGGGAGCCC CCCCTATGCTGCTCCCGAAGTCTTTGAGGGGAAGGAGTATGAAGGTCCCCAGCTGGACATCTGGAGCCTC GGCGTGGTGCTGTATGTCCTGGTCTGTGGCTCCCTCCCTTTTGATGGACCCAACCTGCCTACGCTGAGAC AGCGGGTGCTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGACACTGATCAGACG CATGCTGGTAGTGGACCCCGCCAAGCGCATCACCATTGCCCAGATCCGCCAGCACCGATGGATGCAGGCC GACCCCACCCTCCTGCAGCAGGACGACCCTGCCTTCTCCATGCAGGGCTACACCTCCAACCTGGGCGACT ACAACGAGCAGGTGCTGGGCATCATGCAGGCCCTGGGCATCGACCGGCAGAGGACAGTAGAGTCTCTACA GAACAGCAGCTACAACCACTTTGCGGCTATTTACTACCTCCTGCTCGAGCGCCTCAGGGAGCATCGAAGC ACCCAGCCCTCATCCCGGGCCACCCCTGCACCTGCCAGACAGCCTCAGCTCCGAAACTCAGACCTCAGCA GTCTGGAGGTTCCTCAAGAAATTCTCCCATGTGACCCTTTCCGGCCCTCTCTGCTGTGCCCGCAGCCCCA GGCCTTGGCTCAGTCTGTCCTGCAGGCTGAGATAGACTGTGATCTCCACAGTTCGCTTCAGCCCTTATTC TTCCCCCTGGATACCAACTGCAGCGGAGTGTTCCGGCACCGATCCATCTCCCCCAGCAGCCTGCTGGACA CAGCTATCAGTGAGGAGGCCAGGCAGGGTCCCAGCCTGGAGGAGGAGCAGGAGGTCCAGGAACCCCTGCC TGGCAGCACTGGCCGGAGGCATACACTGGCTGAAGTTTCCACCCACTTCTCCCCGCTCAACCCTCCTTGC ATAATTGTCTCCTCCTCTGCCGCTGTGAGTCCCTCGGAAGGAACTAGCTCTGACAGCTGCCTCCCCTTCT CTGCAAGCGAAGGTCCTGCAGGGCTTGGTGGTGGCCTGGCCACCCCAGGGCTTCTGGGCACCAGCTCTCC AGTCAGATTGGCCTCACCCTTCCTGGGATCACAGTCAGCCACCCCTGTGCTACAGAGTCAGGCAGGTCTG GGTGCGACTGTCTTACCTCCTGTCAGCTTCCAGGAGGGACGGAGAGCGTCGGATACGTCTCTCACTCAAG GGCTGAAGGCCTTCCGGCAGCAGCTAAGGAAAAACGCGAGGACCAAGGGGTTCCTGGGACTGAACAAGAT CAAAGGATTGGCCCGTCAGGTGTGCCAGTCCTCCATCCGAGGTTCCCGGGGAGGGATGAGTACCTTCCAC ACCCCGGCCCCAAGCTCAGGTCTGCAAGGCTGCACGGCCAGCAGTCGCGAGGGCAGGAGCCTGCTCGAAG AGGTGCTGCACCAGCAGAGGCTGCTCCAGTTACAGCACCACTCAGCCGTCTCATCTGACTACCAGCAGGC CCCCCAACTGTCCCCCGTCCCCTACGTGCTCACTCCCTGTGATGGCCTTCTCGTGTCTGGAATCCCACTG CTGCCAACGCCCCTCCTCCAGCCCGGCATGTCCCCCGTGGCGTCTGCCGCGCAGCTCCTGGATGCCCACT TGCACATCAGTGCTGGCCCAGTGGCCCTCCCCACTGGTCCCCTGCCACAGTGCCTCACCAGGCTGTCCCC AAGCTGTGATCCTGCTGGGCTGCCGCAGGGGGATTGTGAGATGGAGGACCTGACCTCTGGCCAGCGGGGG ACGTTCGTCCTGGTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206846 representing NM_021693
Red=Cloning site Green=Tags(s) MVIMSEFSAVPTGTGQGQQKPLRVGFYDVERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEK IYREVQLMKLLNHPNIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYC HNHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKPGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSL GVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCETLIRRMLVVDPAKRITIAQIRQHRWMQA DPTLLQQDDPAFSMQGYTSNLGDYNEQVLGIMQALGIDRQRTVESLQNSSYNHFAAIYYLLLERLREHRS TQPSSRATPAPARQPQLRNSDLSSLEVPQEILPCDPFRPSLLCPQPQALAQSVLQAEIDCDLHSSLQPLF FPLDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPC IIVSSSAAVSPSEGTSSDSCLPFSASEGPAGLGGGLATPGLLGTSSPVRLASPFLGSQSATPVLQSQAGL GATVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSIRGSRGGMSTFH TPAPSSGLQGCTASSREGRSLLEEVLHQQRLLQLQHHSAVSSDYQQAPQLSPVPYVLTPCDGLLVSGIPL LPTPLLQPGMSPVASAAQLLDAHLHISAGPVALPTGPLPQCLTRLSPSCDPAGLPQGDCEMEDLTSGQRG TFVLVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021693 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021693.2, NP_067725.2 |
| RefSeq Size | 2650 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 59329 |
| UniProt ID | Q9R1U5 |
| MW | 84.9 kDa |
| Gene Summary | inhibits CREB binding and transcription of cAMP-responsive element (CRE)-dependent genes Cyp11A and Star [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206846 | Sik1 (untagged ORF) - Rat SNF1-like kinase (Snf1lk), (10 ug) |
CNY 6,264.00 |
|
| RR206846L3 | Lenti ORF clone of Sik1 (Myc-DDK-tagged ORF) - Rat SNF1-like kinase (Snf1lk), (10 ug) |
CNY 11,780.00 |
|
| RR206846L4 | Lenti ORF clone of Sik1 (mGFP-tagged ORF) - Rat SNF1-like kinase (Snf1lk), (10 ug) |
CNY 12,920.00 |
