Alg6 (NM_001033709) Rat Tagged ORF Clone
CAT#: RR207072
- TrueORF®
Alg6 (Myc-DDK-tagged ORF) - Rat asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg6), (10 ug)
"NM_001033709" in other vectors (3)
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CNY 6,560.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC124652 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207072 representing NM_001033709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCTGGACCTGGATGACAGTCGTGGTCTTACTTGGACTCACGGTGCGGTGGACGGTTTCTCTCA ATTCTTACTCAGGTGCTGGAAAACCGCCTATGTTTGGTGATTATGAAGCTCAGAGACACTGGCAAGAAAT TACCCTTAACTTACCAGTCAAACAATGGTATTTTAACAGTAGTGATAACAATTTACAGTACTGGGGATTG GATTACCCACCTCTTACGGCCTATCACAGTCTCTTATGTGCATATGTGGCAAAGTTTATAAACCCAGACT GGGTTGCTCTCCACACATCACGAGGCTATGAGAGTCAGGCACACAAGCTCTTCATGCGTACCACAGTTCT CGCTGCTGATTTGCTCATTTATATACCCGCAGTGCTTTTGTACTGTTACTCCTTAAAAGAAATCTCACCT AAGAGAAAGATCGCCAGCGCGCTATGCATATTGCTGTACCCAGGTCTCATTCTTATCGACTATGGACATT TTCAGTATAATTCTGTGAGTCTTGGCTTTGCTCTGTGGGGTGTTCTTGGAGTGTCTTGTGACTGGGACCT GCTAGGGTCATTGGCCTTTTGCTTAGCTCTAAATTATAAACAGATGGAGCTTTACCACTCCCTACCATTT TTTTGCTTTTTACTTGGCAAATGTTTTAAAAAAGGCCTCAGAGGAAAGGGGTCTGCATTGTTCATTAGGA TAGCCTGTACTGTCGTGGCCTCCTTCCTTCTGTGTTGGCTGCCGTTCTTGACAGAAAGGGAGCATGCCCT GCAGGTCGTGAGAAGACTCTTCCCTGTTGATCGCGGATTGTTTGAGGATAAAGTAGCCAATATTTGGTGC AGCCTTAATGTTTTTCTGAAGATTAAAGACATTTTGCCTCGTCACATCCAGATAGCAATCAGCTTTTGCT TCACATTTTTGAGCTTGCTTCCTGCATGCATAAAGTTGACAGTTCAGCCCTCTGCTAAAGGATTCAGATT CACTCTGGTTAGCTGTGCACTATCATTCTTTCTGTTTTCTTTCCAAGTACACGAGAAGTCCATTCTCTTG GTATCACTACCAGTCTGCTTAGTTTTAACTGAAATTCCTTTCATGTCAACTTGGTTTTTACTAGTTTCAA CGTTTAGCATGCTGCCTCTTCTATTAAAGGACCAACTCCTGCTGCCCTCGGTTGTGACAGTGATGGCGTT TTTAATAGCTTGTTCCACCTTCTTCCCAATGTTTGAAAACACTTCTGAGGAACAACTGCAGCTGAAGTCC TTTGCCGTCTCTGTGAGGAGGCACCTTCCGGGCTTCACGTTTCTTCCCAGAATTATACAGTGTTTGTTTC TTTCTTCAGTCATCACCATGATCCTTCTGACGATTTTGAGTGTAACACTGGACCCTCCTCAGAAATTACC AGACTTGTTTTCTGTACTTATATGTTTTGTGTCCTGTGTAAACTTTGTATTCTTCTTGGTATATTTTAAC ATTGTAATCATGTGGGATTCCAAAAATGGAAGAAATCGTAAGAAAATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207072 representing NM_001033709
Red=Cloning site Green=Tags(s) MESWTWMTVVVLLGLTVRWTVSLNSYSGAGKPPMFGDYEAQRHWQEITLNLPVKQWYFNSSDNNLQYWGL DYPPLTAYHSLLCAYVAKFINPDWVALHTSRGYESQAHKLFMRTTVLAADLLIYIPAVLLYCYSLKEISP KRKIASALCILLYPGLILIDYGHFQYNSVSLGFALWGVLGVSCDWDLLGSLAFCLALNYKQMELYHSLPF FCFLLGKCFKKGLRGKGSALFIRIACTVVASFLLCWLPFLTEREHALQVVRRLFPVDRGLFEDKVANIWC SLNVFLKIKDILPRHIQIAISFCFTFLSLLPACIKLTVQPSAKGFRFTLVSCALSFFLFSFQVHEKSILL VSLPVCLVLTEIPFMSTWFLLVSTFSMLPLLLKDQLLLPSVVTVMAFLIACSTFFPMFENTSEEQLQLKS FAVSVRRHLPGFTFLPRIIQCLFLSSVITMILLTILSVTLDPPQKLPDLFSVLICFVSCVNFVFFLVYFN IVIMWDSKNGRNRKKID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033709 |
ORF Size | 1521 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033709.1, NP_001028881.1 |
RefSeq Size | 3007 bp |
RefSeq ORF | 1524 bp |
Locus ID | 362547 |
UniProt ID | Q3T1L5 |
MW | 57.9 kDa |
Gene Summary | Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man(9)GlcNAc(2)-PP-Dol (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207072 | Alg6 (untagged ORF) - Rat asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg6), (10 ug) |
CNY 6,560.00 |
|
RR207072L3 | Lenti ORF clone of Alg6 (Myc-DDK-tagged ORF) - Rat asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg6), (10 ug) |
CNY 8,460.00 |
|
RR207072L4 | Lenti ORF clone of Alg6 (mGFP-tagged ORF) - Rat asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg6), (10 ug) |
CNY 9,220.00 |