Pik3c2b (NM_001105951) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207150 representing NM_001105951 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCGACTCAGGGCAATGGGGAGCACTGGAAATCCCTGGAGTCGGTGGGCATCAGCCGCAAAGAGC TGGCTATGGCTGAAGCCCTGCAGATGGAGTATGATGCTCTTTCCCGGCTCCGACATCACAAGGAGGAGAG CAGGGCCAAGCAAAACACAGAGCCCTCTCTTATCAGCTGGGATGAGCCAGCCTTGGACTTCTACAGCAAG CCAGCAGGCAGGCGGACAGAGCTCAAGCTCTTGCGTGGTCTTTCTGGCTCTGATCCCACCCTAAATTACA ACTCCATTTCCCCACCAGAAGAGCTCCCCAACTCTACCTCCCAGGACCCACAGCCTGGCACAGATCCCTG GCCCAAGGGCTCCCAATCTGGAGACTATCTCTATATTTTTGATGGCTCAGATGGGAGATGCTCTCTGTCT CCAGTATCAGGTGACACCGATGGCTCTTGTAAGAAACTATCCCCACCTCCTCTCCCTCCTCGGGTCTCTA TCTGGGATGCCCCTCCCTTGCCTCCTAGGAAGGGATCTCCCTCACCCTCCAAGATCTCTCAGCCTGATGA CATCAACAGTTTCTCTTTGGTGGAACAGCCATCTGACAAACTGCTAGGGGCCCAGGACCCAGGAGAGGGA GAGCTACCCAATGGTGGGGGACAGCGACATGTGCTGGGGTCCGTGGACTATGACGGCATCAATGATGCAA TCACGAGGCTCAACTTGAAGTCCACTTACGATTCGGAGATATTGTCTGACGCTACCAGGGGCTGGAAGGA GGGCCGAGGACCCCTGGACTTTAGCAAGGACACCTCTGGGAAACCTGTAGCCCGGAGCAAGACGATGCCC CCTCAAGTGCCCCCTCGAACCTATACGCCCCGATACGCCAACCGAAAAAACGCAACACCTGGCAAGAACC GCCGGATATCTGCCGCTCCGGTGAGCTCTCGGACCCACACTGTCACCAATGGCCATGAATTGTTTGAGGT CTCAGAGGAGAGAGATGAGGAAGTTGCTGCGTTCTGCCACATGCTAGATATCCTGCGCACGGGCTCTGAT ACCCAGGACTATTCCCTAACTGGGTGTGTCTGGAGCACTGTCACCCCGAGCCCAGAGCACCTTGGGGATG AGGTCAACCTGAAGGTGACTGTGTTGTGTGACAGTCTTCGGGAGCCACTCACCTTTACCTGCAACTGTTC CTCCACCGTGGACTTGCTCATTTACCAGACCCTCTGCTACACCCACGACGAGCTGAGGGAGGTGGATGTG GGCGACTTTGTGCTCAAGCCCTGTGGGCTGGAGGAATTCCTGCAAAATAAGCACGCCTTGGGAAGCCATG AGTATATCCAACACTGCCGCAAGTTTGACATCAACATCCGACTACAGCTGATGGAGCAGAAGGCTATCCG GAGCGACCTGGCCCGGACGGTGAATGATGACCAGAGCCCTTCCACCTTGAACTACCTCATCCATCTTCAA GAGCGGCCAGTCAAGCAGACCATCAGCAGGCAGGCCTTGAGTCTTCTGTTTGACACTTACCACAATGAGG TGGATGCCTTCCTACTGGCTGAAGGGGATTTCCCACTGAAGGCTGACAGGGTGGTCCAGTCTGTGAAGGC CATCTGCAATGCTCTGGCTGCTGTGGAGACTCCTGAGATCACGAGTGCTCTCAACCAGCTGCCTCCCTGC CCGTCACGAATGCAGCCGAAAATCCAGAAGAAGGTGGTAGAAGCCTTGACAGCAGCCATCTTGGACTTAG TGGAGCTGTACTGCAGCACATTCAATGCAGACTTCCAGACAGCCGTACCTGGGAGCCGAAAGCATGATTT GGTTCAAGAGGCCTGCCACTTCTCTGGGGCCCTGGCCTTCACTGTCTATGGCACTCACCGTATTCCCATC ATCTGGGCTACCAGCTATGAAGAGTTCTACCTCTCCTGCTCCCTCAGCCACGGTGGCAAGGAACTTTGCA GCCCTCTGCAGACCCGGCGGGCTCACTTCTCCAAGTACCTGTTCCACCTCATCATCTGGGACCAGCAGAT CTGCTTTCCCGTGCAGGTGAACAGGCTGCCTCGGGAGACCCTGCTGTGTGCCACACTCTATGCCCTGCCC ATTCCCCCTCCCGGGAGTTCCTCCGAAGCCAATAAGCAGAAGCGGGTGCCTGAAGCTCTGGGTTGGGTCA CTACCCCCCTCTTCAACTTCAGGCAAGTCTTGACCTGTGGCCGAAAGCTTCTGGGCTTGTGGCCAGCAAC ACAGGAGAACTCCAGTGCCCGTTGGAGTGCACCAAATTTCCACCAGCCAGACAGTGTCATCCTGCAGATT GACTTCCCCACCTCGGCCTTTGACATCAAGTTCACCAGCCCCCCTGGAGACAAGTTCAGCCCCCGCTATG AGTTTGGCAGTCTCCGGGAGGAGGACCAGCGCAAACTTAAAGACATTACGCAGAAGGAGTCCCTTTACTG GCTCTCTGATGCCGACAAGAAGCAACTGTGGGAGAAGCGCTATTACTGCCACACTGAGGTGAGCTCGCTC CCCTTGGTGCTCGCCAGTGCACCCAGCTGGGAGTGGGCTTGCCTGCCGGACATCTATGCTCTCCTGCAAC AGTGGACTCACATGAACCACCAGGATGCCCTGGGACTCCTGCATGCCACCTTCCCGGACCAGGAGGTGCG GCGCATGGCCGTGCAGTGGATCGGCTCCCTCTCGGATGCTGAGCTGTTGGACTACCTGCCCCAGCTAGTA CAGGCCCTGAAGTACGAGTGCTACCTGGATAGCCCCTTGGTGCGCTTTCTCCTGAAGCGAGCCATCTCCG ACCTGCGAGTGACCCACTACTTTTTCTGGTTACTGAAGGACAGCCTCAAGGACTCCCAGTTCAGCATCCG TTACCAATACCTGCTAGCGGCCCTGCTGTGCTGCTGTGGCAAGGGACTGAGGGAGGAGTTTAACCGCCAG TGCTGGCTTGTCAATACCCTGGCCAAACTGGCCCAGCAGGTCCGAGAGGCCACCCCATCTGCACGCCAGG GCATCCTTCGTGCGGGCTTGGAGGAGGTGAGGCAGTTCTTTGCTCTCAATGGTTCGTGCCGTCTGCCGCT TAGCCCCAGTCTGCTGGTGAAAGGCATTGTGCCCAGGGACTGTTCCTATTTCAACTCCAATGCTGTCCCC CTCAAGCTTGCCTTCCAAAATGTGGATCCACTGGGCGAGAATATCAGAGTCATCTTCAAGTGTGGAGATG ACCTTCGCCAGGACATGCTCACCCTGCAGATGATTCGCATCATGAGCAAGATCTGGGTGCAGGAGGGGCT GGATATGCGCATGGTCATTTTCCGCTGCTTCTCCACTGGCCGAGGAAAAGGGATGGTGGAGATGATACCC AATGCCGAGACACTGCGTAAGATCCAGGTGGAGCATGGGGTGACCGGCTCCTTCAAGGACCGTCCCCTGG CTGACTGGCTTCAGAAGCACAACCCTGGGGAGGATGAATATGAAAAGGCTGTGGAGAACTTCATCTACTC TTGTGCCGGCTGCTGTGTGGCTACATACATCTTGGGCATCTGTGACAGACACAATGACAACATCATGCTG AAGACCACGGGCCACATGTTCCATATAGACTTCGGCCGCTTCCTGGGCCACGCGCAGATGTTCGGTAATA TCAAGAGGGACCGCGCCCCGTTTGTCTTCACCTCAGACATGGCGTATGTCATCAACGGGGGTGACAAGCC TTCCAGCCGCTTCCATGATTTTGTTGACCTTTGCTGCCAAGCCTACAACCTCATTCGCAAGCACACCCAT CTCTTCCTCAACCTCCTGGGCCTGATGTTGTCCTGTGGGATCCCCGAGCTCTCAGACCTGGAGGACCTCA AGTATGTGTATGATGCCCTGAGACCTCAGGACACGGAGGCCAATGCCACTACCTACTTCACAAGGTTGAT TGAGTCCAGCTTGGGCAGCGTAGCTACGAAACTCAACTTTTTCATCCACAACCTGGCTCAGATGAAGTTC ACAGGCTCAGACGACCGGCTCACCCTCTCCTTTGCCCCCCGAACACATACTCTCAAGAGCTCTGGCCGCA TCCGTGATGTCTTTCTCTGCCGACATGAGAAGGTCTTCCACCCTAGCAAGGGCTATATCTATGTGGTGAA GGTGATGCGAGAGAACGCTCACGAGGCAACCTACATCCAGAGGACGTTCGAGGAGTTCCAGGAACTGCAC AATAAGCTGCGCCTGCTCTTCCCTTCCTCCTTCCTGCCCAGCTTCCCTAGTCGGTTTGTGATTGGCCGCT CCCGGGGAGAGGCAGTGGCTGAGAGGCGGAAGGAGGAGCTAAATGGCTACATCTGGCACTTGATTCATGC AGCCCCTGAAGTGGCTGAGTGTGACCTGGTATATACTTTCTTCCATCCTCTGCCCCGCGATGAGAAGACT TCAGGCCCTAGCCCAGCCCCGAAGTCATCAGATGGGACGTGGGCCCGGCCGGTTGGGAAGGTCGGTGGGG AGGTGAAGCTGTCCATTTCCTACAAAAACAACAAACTCTTCATCATGGTGATGCATATTCGGGGCTTGCA ACCGCTCCAGGATGGCAGTGACCCTGACCCATATGTAAAGATTTACCTCCTTCCTGACCCTCAGAAAGCC ACAAAGAGGAAAACCAAAGTAGCCCGGAAAACCTGCAACCCTACCTACAATGAGATGTTGGTGTATGATG GGATTCCCAAGGGAGACCTACAACAGCGGGAGCTCCAGTTGAGTGTGCTAAGTGAGCAAGGATTTTGGGA GAACGTGCTTCTCGGGGAGGTGCACATCCGCCTTCGGGAGCTGGACCTGGCCCAGGAGAAGACCGGCTGG TTTGCACTGGGATCTCGAGGTCATGGAACCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207150 representing NM_001105951 Red=Cloning site Green=Tags(s) MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSK PAGRRTELKLLRGLSGSDPTLNYNSISPPEELPNSTSQDPQPGTDPWPKGSQSGDYLYIFDGSDGRCSLS PVSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPDDINSFSLVEQPSDKLLGAQDPGEG ELPNGGGQRHVLGSVDYDGINDAITRLNLKSTYDSEILSDATRGWKEGRGPLDFSKDTSGKPVARSKTMP PQVPPRTYTPRYANRKNATPGKNRRISAAPVSSRTHTVTNGHELFEVSEERDEEVAAFCHMLDILRTGSD TQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIYQTLCYTHDELREVDV GDFVLKPCGLEEFLQNKHALGSHEYIQHCRKFDINIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQ ERPVKQTISRQALSLLFDTYHNEVDAFLLAEGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPC PSRMQPKIQKKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFSGALAFTVYGTHRIPI IWATSYEEFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALP IPPPGSSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSSARWSAPNFHQPDSVILQI DFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLSDADKKQLWEKRYYCHTEVSSL PLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLV QALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQ CWLVNTLAKLAQQVREATPSARQGILRAGLEEVRQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVP LKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIP NAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIML KTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTH LFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKF TGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKVFHPSKGYIYVVKVMRENAHEATYIQRTFEEFQELH NKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKT SGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGSDPDPYVKIYLLPDPQKA TKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVHIRLRELDLAQEKTGW FALGSRGHGTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105951
ORF Size	4863 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105951.1, NP_001099421.1
RefSeq Size	5340 bp
RefSeq ORF	4866 bp
Locus ID	289021
MW	183.1 kDa

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