Sae1 (NM_001012063) Rat Tagged ORF Clone
CAT#: RR207212
- TrueORF®
Sae1 (Myc-DDK-tagged ORF) - Rat SUMO1 activating enzyme subunit 1 (Sae1), (10 ug)
"NM_001012063" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Uble1a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207212 representing NM_001012063
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGAAGGAGGAGGTCAGCGGCGGCGGCGGCGGCATCAGCGAGGAGGAGGCAGCGCAGTATGACC GACAGATCCGCCTGTGGGGACTGGAGGCTCAGAAGCGGCTTCGAGCTTCTCGGGTACTGATTGTCGGCAT GAAAGGACTTGGGGCTGAAATCGCCAAGAACCTTATCCTGGCAGGAGTGAAAGGGCTCACCATGCTGGAC CATGAACAGGTATCTCCAGAAGACCTTGGAGCTCAGTTCTTGATTCGAACTGGGTCTGTGGGCCAAAACA GGGCCGAGGCCTCTTTGGAACGAGCCCAGAATCTCAACCCCATGGTGGATGTAAAGGTGGACACTGAGGA TATAGAGAAGAAGCCAGAGTCCTTCTTCACAGAGTTTGATGCGGTGTGCCTGACTTGCTGCTCCAAAGAT GTCATCATTAAAGTCGACCAGATCTGTCACAGAAATAGCATCAAGTTCTTCACAGGAGATGTCTTTGGGT ACCATGGGTACACCTTTGCAAATCTTGGAGAGCATGAATTTGTAGAAGAGAAAACCAAAGTTACCAAAGT CAGCCAAGGAGTGGAAGATGGACCTGATGCCAAGAGAGCAAAGCTTGACTCATCGGAGACCACCATGGTC AAAAAGAAAGTGCTTTTCTGCCCTGTAAAGGAAGCGCTAGCAGTGGACTGGAGTGGAGAGAAAGCCCAGG CTGCCCTGAAGCGCACTGCCCCAGACTACTTTCTGCTGCAAGTGTTGCTGAAGTTCCGCACGGATAAAGG GAGAGACCCTACTTCTGACAGCTACAGCGAGGATGCCGAGCTGCTGCTGCAGATACGCAATGACGTGTTT GACTCGCTGGGCGTCAGTCCTGACCTGCTTCCCGATGACTTTGTCAGGTACTGCTTCTCTGAGATGGCCC CAGTATGTGCTGTGGTTGGAGGAATCTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAAAGGGACCCTCC TCACAACAACTTCTTCTTCTTCGATGGCATGAAGGGGAGTGGAATTGTGGAGTGCCTTGGTCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207212 representing NM_001012063
Red=Cloning site Green=Tags(s) MVEKEEVSGGGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLD HEQVSPEDLGAQFLIRTGSVGQNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTEFDAVCLTCCSKD VIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVTKVSQGVEDGPDAKRAKLDSSETTMV KKKVLFCPVKEALAVDWSGEKAQAALKRTAPDYFLLQVLLKFRTDKGRDPTSDSYSEDAELLLQIRNDVF DSLGVSPDLLPDDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001012063 |
ORF Size | 1047 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001012063.1, NP_001012063.1 |
RefSeq Size | 1914 bp |
RefSeq ORF | 1050 bp |
Locus ID | 308384 |
UniProt ID | Q6AXQ0 |
MW | 38.5 kDa |
Gene Summary | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207212 | Sae1 (untagged ORF) - Rat SUMO1 activating enzyme subunit 1 (Sae1), (10 ug) |
CNY 3,990.00 |
|
RR207212L3 | Lenti ORF clone of Sae1 (Myc-DDK-tagged ORF) - Rat SUMO1 activating enzyme subunit 1 (Sae1), (10 ug) |
CNY 6,080.00 |
|
RR207212L4 | Lenti ORF clone of Sae1 (mGFP-tagged ORF) - Rat SUMO1 activating enzyme subunit 1 (Sae1), (10 ug) |
CNY 6,650.00 |