Chek1 (NM_080400) Rat Tagged ORF Clone
CAT#: RR207551
- TrueORF®
Chek1 (Myc-DDK-tagged ORF) - Rat CHK1 checkpoint homolog (S. pombe) (Chek1), (10 ug)
"NM_080400" in other vectors (3)
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CNY 6,180.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207551 representing NM_080400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCTTTTGTGGAAGACTGGGATTTGGTGCAAACTTTGGGAGAAGGCGCCTATGGAGAAGTTC AACTTGCTGTGAATAGAATAACTGAAGAAGCTGTTGCAGTGAAAATTGTAGACATGAAGCGGGCCATAGA CTGCCCAGAAAATATTAAGAAAGAGATCTGCATCAATAAGATGTTAAATCATGAGAATGTAGTGAAATTC TATGGCCACAGGAGGGAAGGCAACATCCAGTACCTGTTTCTGGAGTACTGTAGTGGAGGAGAACTCTTTG ATAGAATAGAGCCAGACATAGGGATGCCTGAACAAGATGCTCAGAGGTTTTTCCATCAGCTCATGGCGGG GGTGGTTTATCTTCATGGCATTGGAATAACTCACAGGGATATTAAACCAGAAAACCTCCTCTTGGATGAA AGGGATAATCTCAAAATCTCTGACTTTGGCTTGGCAACAGTGTTTCGGCATAATAATCGTGAGCGCTTAC TGAACAAGATGTGTGGGACTTTACCTTATGTCGCTCCAGAGCTTCTAAAGAGGAAAGAATTTCACGCAGA ACCAGTTGATGTTTGGTCCTGTGGAATAGTGCTTACTGCAATGTTGGCTGGAGAATTGCCTTGGGACCAG CCCAGTGATAGCTGTCAAGAATATTCTGATTGGAAAGAAAAGAAAACCTATCTCAATCCTTGGAAAAAAA TTGATTCTGCTCCTCTGGCTTTGCTTCATAAAATTCTAGTTGAGAATCCATCAGCAAGGATCACCATCCC AGACATTAAAAAAGATAGATGGTATAACAAACCACTTAACAGAGGAGCGAAGAGGCCACGTGCTACTTCA GGTGGTATGTCAGAGTCTTCTAGTGGATTCTCTAAGCACATTCATTCCAATTTGGACTTTTCTCCAATAA ATAGTGGTTCCAGTGAAGAAAACGTGAAGTTCTCTAGTTCCCAGCCAGAGCCTCGGACAGGGCTTTCCTT GTGGGACACTGGCCCCTCAAATGTGGACAAACTGGTGCAAGGCATCAGTTTTTCCCAGCCTACGTGTCCT GATCATATGCTTGTAAACAGTCAGCTACTTGGTACCCCTGGATCTTCACAGAACCCCTGGCAGCGCTTGG TCAAAAGGATGACACGATTCTTCACTAAATTGGATGCAGACAAATCTTACCAATGCCTGAAAGAGACCTT TGAGAAGTTGGGCTATCAGTGGAAGAAGAGTTGTATGAATCAGGTTACTGTATCAACAATGGATAGAAGA AACAATAAGTTGATTTTCAAAATAAATTTGGTAGAAATGGATGAGAAGATACTGGTTGACTTCCGGCTTT CTAAGGGTGATGGGTTAGAGTTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTCAGCGATATCGTGAG CAGCCAGAAGGTTTGGTTCCCTGTTACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207551 representing NM_080400
Red=Cloning site Green=Tags(s) MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLNHENVVKF YGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDE RDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQ PSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRPRATS GGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCP DHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRR NNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDIVSSQKVWFPVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080400 |
ORF Size | 1428 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080400.1, NP_536325.1 |
RefSeq Size | 2370 bp |
RefSeq ORF | 1431 bp |
Locus ID | 140583 |
UniProt ID | Q91ZN7 |
MW | 54.4 kDa |
Gene Summary | protein kinase that can bind to single-stranded DNA; may function in DNA replication and repair [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207551 | Chek1 (untagged ORF) - Rat CHK1 checkpoint homolog (S. pombe) (Chek1), (10 ug) |
CNY 6,180.00 |
|
RR207551L3 | Lenti ORF clone of Chek1 (Myc-DDK-tagged ORF) - Rat CHK1 checkpoint homolog (S. pombe) (Chek1), (10 ug) |
CNY 7,980.00 |
|
RR207551L4 | Lenti ORF clone of Chek1 (mGFP-tagged ORF) - Rat CHK1 checkpoint homolog (S. pombe) (Chek1), (10 ug) |
CNY 8,840.00 |