Asic1 (NM_024154) Rat Tagged ORF Clone
CAT#: RR207637
- TrueORF®
Asic1 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 2, neuronal (Accn2), (10 ug)
"NM_024154" in other vectors (3)
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CNY 6,750.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Accn2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207637 representing NM_024154
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATTGAAGACCGAGGAGGAGGAGGTGGGTGGTGTCCAGCCGGTGAGCATCCAGGCTTTCGCCAGCA GCTCCACGCTGCATGGTCTTGCCCACATCTTCTCCTATGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGC CCTGTGCTTCCTGGGTTCGCTGGCCGTCCTGCTGTGTGTGTGCACTGAGCGTGTGCAGTACTACTTCTGC TATCACCACGTCACCAAGCTTGACGAAGTGGCTGCCTCCCAGCTCACCTTCCCTGCTGTCACACTGTGCA ATCTCAATGAGTTCCGCTTTAGCCAAGTCTCCAAGAATGACCTGTACCATGCTGGGGAGCTGCTGGCCCT GCTCAACAACAGGTATGAGATCCCGGACACACAGATGGCTGATGAAAAGCAGCTAGAGATATTGCAGGAC AAGGCCAACTTCCGGAGCTTCAAGCCCAAGCCCTTCAACATGCGTGAATTCTACGACAGAGCGGGGCACG ATATTCGAGACATGCTGCTCTCGTGCCACTTCCGTGGGGAGGCCTGCAGCGCTGAAGATTTCAAAGTGGT CTTCACTCGGTATGGGAAGTGTTACACATTCAACTCGGGCCAAGATGGGCGGCCACGGCTGAAGACCATG AAAGGTGGGACTGGCAATGGCCTGGAGATCATGCTGGACATTCAGCAAGATGAATATTTGCCTGTGTGGG GAGAGACCGACGAGACATCCTTCGAAGCAGGCATCAAAGTGCAGATCCACAGTCAGGATGAACCCCCTTT CATCGACCAGCTGGGCTTTGGTGTGGCTCCAGGTTTCCAGACGTTTGTGTCTTGCCAGGAGCAGAGGCTC ATCTACCTGCCCTCACCCTGGGGCACCTGCAATGCTGTTACCATGGACTCGGATTTCTTCGACTCCTACA GCATCACTGCCTGCCGGATTGATTGCGAGACGCGTTACCTGGTGGAGAACTGCAACTGCCGTATGGTGCA CATGCCAGGGGACGCCCCATACTGCACTCCAGAGCAGTACAAGGAGTGTGCAGATCCTGCCCTGGACTTC CTAGTGGAGAAAGACCAGGAATACTGCGTGTGTGAGATGCCTTGCAACCTGACCCGCTACGGCAAGGAGC TGTCCATGGTCAAGATCCCAAGCAAAGCCTCCGCCAAGTACCTGGCCAAGAAGTTCAACAAATCGGAGCA GTACATAGGGGAGAACATTCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATCGAGCAG AAAAAGGCCTATGAGATCGCAGGGCTGTTGGGTGACATCGGGGGCCAGATGGGGTTGTTCATCGGTGCCA GCATCCTCACCGTGCTGGAACTCTTTGACTATGCCTACGAGGTCATTAAGCACAGGCTGTGCAGACGTGG AAAGTGCCAGAAGGAGGCTAAGAGGAGCAGCGCAGACAAGGGCGTGGCGCTCAGCCTGGATGACGTCAAA AGACACAATCCCTGCGAGAGCCTCCGAGGACATCCTGCCGGGATGACGTACGCTGCCAACATCCTACCTC ACCATCCCGCTCGAGGCACGTTTGAGGACTTTACCTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR207637 representing NM_024154
Red=Cloning site Green=Tags(s) MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFC YHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQD KANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTM KGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRL IYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDF LVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQ KKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRSSADKGVALSLDDVK RHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024154 |
ORF Size | 1578 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024154.2, NP_077068.1 |
RefSeq Size | 3771 bp |
RefSeq ORF | 1581 bp |
Locus ID | 79123 |
UniProt ID | P55926 |
MW | 59.6 kDa |
Gene Summary | forms a proton-gated ion channel that may play a role in sensory neuron function and pain perception [RGD, Feb 2006] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Dual actions of Psalmotoxin at ASIC1a and ASIC2a heteromeric channels (ASIC1a/2a)
,Liu, Y;Hagan, R;Schoellerman, J;,
Sci Rep
,PubMed ID 29739981
[ASIC1]
|
Regulation of breast tumorigenesis through acid sensors
,null,
Oncogene
,PubMed ID 26686084
[Asic1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207637 | Asic1 (untagged ORF) - Rat amiloride-sensitive cation channel 2, neuronal (Accn2), (10 ug) |
CNY 5,888.00 |
|
RR207637L3 | Lenti ORF clone of Asic1 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 2, neuronal (Accn2), (10 ug) |
CNY 8,650.00 |
|
RR207637L4 | Lenti ORF clone of Asic1 (mGFP-tagged ORF) - Rat amiloride-sensitive cation channel 2, neuronal (Accn2), (10 ug) |
CNY 9,500.00 |