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Wasf1 (NM_001025114) Rat Tagged ORF Clone
CAT＃: RR207646
- TrueORF®
Wasf1 (Myc-DDK-tagged ORF) - Rat WAS protein family, member 1 (Wasf1), (10 ug)
"NM_001025114" in other vectors (3)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC109303 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207646 representing NM_001025114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGGTGAAAAGAAACATCGACCCTAGGCACTTGTGCCACACAGCACTGCCCAGGGGCATTAAGA ACGAGCTGGAATGTGTGACCAACATCTCCCTGGCAAATATAATTAGACAACTGAGTAGCCTAAGTAAATA TGCTGAAGACATATTTGGAGAATTATTCAATGAAGCACATAGTTTTTCCTTCAGAGTTAACTCATTACAA GAGCGCGTGGACCGATTGTCCGTTAGTGTCACACAGCTTGATCCTAAAGAAGAAGAACTGTCCTTACAAG ACATAACGATGAGAAAAGCTTTCCGGAGCTCTACAATTCAGGACCAGCAGCTTTTCGACCGCAAGACTTT GCCTATCCCGCTGCAGGAAACGTACGATGTGTGCGAGCAGCCTCCGCCTCTCAATGTCCTCACACCCTAC AGGGACGATGGTAAGGAGGGGCTGAAGTTTTATACCAACCCTTCGTATTTCTTTGATCTGTGGAAAGAAA AGATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGAAAGCAGAAGCAGAAAAATCTAGACCGTCC TCATGAACCAGAAAAAGTGCCAAGAGCACCACATGACAGGCGGCGAGAATGGCAGAAGCTGGCCCAAGGT CCAGAGCTGGCTGAAGATGATGCCGATCTCCTCCACAAGCACATCGAAGTTGCTAACGGCCCAGCCTCTC ATTTTGAGACAAGGCCGCAGACATACGTGGACCACATGGATGGATCTTACTCACTCTCTGCCTTGCCATT CAGTCAGATGAGTGAACTCCTAAGCAGAGCTGAGGAGAGGGTCTTAGTCAGACCCCATGAACCACCTCCA CCTCCACCAATGCATGCAGCAGGGGATGCAAGACCCACCCCCACCTGTGTCAGCTCTGCTGCGGGTTTGA TAGAGAATCGTCCCCAGTCCCCAGCTGCAGGCAGAACACCCGTGTTTGTGAGCCCCACGCCCCCACCTCC TCCACCACCTCTCCCGTCTGCCCTGTCCACCTCCTCACTGAGAGCTTCCATGACCTCAACTCCTCCCCCA CCGGTGCCTCCCCCGCCTCCTCCTCCAGCCCCTGCCTTGCAGGCCCCAGCAGTGCCCCCGCCTCCAGCCC CTCTTCAGATTGCCCCTGGTGTCCTTCACCCAGCTCCTCCTCCAATCGCTCCTCCTCTAGTACAGCCCTC TCCCCCGGTAGCCAGAGCCGCCCCAGTGTGTGAGACTGTACCCGTTCATCCACTCCCACAAGGTGAAGTG CAGGGGCTGCCTCCGCCCCCGCCGCCGCCGCCTCTGCCTCCTCCAGGCATTCGGCCATCATCACCTGTCA CGGTCGCAGCTCTTGCGCATCCTCCCTCTGGGCTCCACCCAACTCCATCTCCTGCCCCTGGTCCCCATGC TCCATTAATGCCTCCGTCCCCACCTTCACAAGTCCTGCCTGCCTCTGAGCCAAAGCGCCATCCTTCCACC CTACCCGTGATCAGTGACGCGAGGAGTGTGCTGCTGGAGGCCATACGGAAAGGCATTCAGCTTCGCAAAG TGGAAGAGCAGCGTGAACAGGAAGCAAAGCATGAGCGGATTGAAAACGACGTGGCCACCATCCTGTCACG CCGCATCGCTGTGGAGTACAGCGACTCGGAAGACGACTCGGAGTTCGATGAAGTAGACTGGCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207646 representing NM_001025114
Red=Cloning site Green=Tags(s) MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQ ERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPY RDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQG PELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPP PPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPP PVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEV QGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPST LPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025114 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025114.1, NP_001020285.1 |
| RefSeq Size | 2579 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 294568 |
| UniProt ID | Q5BJU7 |
| MW | 61.5 kDa |
| Gene Summary | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207646 | Wasf1 (untagged ORF) - Rat WAS protein family, member 1 (Wasf1), (10 ug) |
CNY 7,130.00 |
|
| RR207646L3 | Lenti ORF clone of Wasf1 (Myc-DDK-tagged ORF) - Rat WAS protein family, member 1 (Wasf1), (10 ug) |
CNY 9,120.00 |
|
| RR207646L4 | Lenti ORF clone of Wasf1 (mGFP-tagged ORF) - Rat WAS protein family, member 1 (Wasf1), (10 ug) |
CNY 9,980.00 |
