Cacna1b (NM_147141) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207906 representing NM_147141 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGCG GCGGGGCCGGCGGGGCCGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAACGCGCACGGACCATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATATGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATATATGATCCTGGCCACCATCATCGCCAACTGTATTGTCCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAACGACTGGATGACACGGAACCTTACTTCATCGGCATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCTCTGGGCTTCGTGTTCCACAAAGGCTCCTACCTCCGGA ATGGCTGGAACGTCATGGACTTCGTGGTGGTCCTCACAGGGATTCTTGCCACAGCTGGAACTGACTTTGA TCTGCGCACCCTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGTTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTCAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATCGGGCTGCTGCTCTTCTTCGCCA TCCTCATGTTCGCTATCATCGGCCTCGAGTTCTATATGGGCAAATTCCATAAGGCCTGCTTCCCCAACAG CACAGATGCAGAGCCTGTGGGTGACTTTCCTTGTGGCAAGGAGGCCCCTGCTCGTCTGTGTGACAGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACCGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC CGGCAACACGTGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTCGGTGTGCTTTCAGGAGAGTTTGCCAAAGAGCGGGAGCGAGTCGAGAACCGCCGTGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAACTGAATGGGTACTTGGAGTGGATCTTCAAGGCGGAGGA AGTCATGTTGGCAGAGGAGGACAAGAACGCAGAAGAGAAGTCCCCTTTGGATGTGTTGAAGAGAGCTGCT ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCTG CTGGGTCTCCCTTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACAGAGAGCTCATCGTACTTCCGGAGGAA GGAGAAGATGTTCCGGTTCCTTATCCGTCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGC GTGGTGGCCCTGAACACGTTGTGTGTGGCCATGGTACACTATAATCAGCCTCAGCGGCTTACCACTGCAC TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTACGGTCTAGG GCCCAGAAGCTACTTCCGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTCGGAATCAGTGTGCTGCGGGCTCTCCGACTGCTGA GGATTTTCAAAGTCACCAAGTATTGGAACTCCCTGAGGAACCTGGTTGTTTCCCTCCTCAACTCCATGAA GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTTTTCATTGTGGTCTTCGCTCTGTTGGGGATGCAGCTG TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC TCACTGTGTTTCAGATTCTGACAGGAGAGGACTGGAATGCAGTCATGTATCATGGGATTGAGTCACAAGG AGGAGTCAGCAAAGGCATGTTTTCATCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG TTGAACGTTTTCTTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG AGATGGAAGAGGCAGCCAATCAGAAGCTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT GTCTGCTGCCAACATCTCCATTGCTGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAG CGGGCCAGTCAGCTAAGGCTCCAGAACCTGCGTGCCAGCTGTGAGGCACTGTACAGTGAGATGGACCCGG AGGAGCGCCTGCGTTATGCCAGCACGCGCCACGTGAGGCCAGACATGAAGACACACATGGACCGACCCCT AGTGGTGGAACCTGGTCGGGATGGCCTGCGGGGACCCGCCGGGAACAAGTCAAAGCCTGAGGGCACGGAG GCCACCGAAGGTGCGGATCCACCACGCCGACACCACCGGCATCGTGATAGGGACAAGACCTCAGCCTCAA CCCCTGCTGGAGGCGAACAGGACAGGACAGACTGCCCAAAGGCCGAAAGCACCGAGACCGGGGCCCGGGA GGAACGTGCGCGCCCTCGTCGAAGTCACAGCAAGGAGGCTCCAGGGGCTGACACACAAGTGCGTTGTGAG CGCAGTAGACGTCACCACCGGCGCGGATCCCCGGAGGAGGCCACTGAACGGGAACCTCGGCGCCACCGTG CCCACCGGCACGCACAGGACTCAAGCAAGGAAGGCAAGGAGGGCACTGCACCGGTGCTTGTACCCAAGGG CGAGCGTCGCGCAAGACATCGAGGCCCGCGTACGGGCCCCCGTGAGACAGAGAACAGTGAGGAGCCCACA CGCAGGCACCGTGCAAAGCATAAGGTGCCACCAACACTTGAGCCCCCAGAGAGGGAGGTTGCAGAGAAGG AGAGCAACGTGGTGGAAGGGGATAAGGAAACTCGAAATCACCAGCCCAAGGAACCTCGCTGTGACCTGGA GGCCATTGCGGTTACAGGCGTGGGCTCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAA CAGCCAGAGGATGCAGACAACCAGCGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGACCCCAGCACCA CTGTGCATGTCCCAGTGACACTGACAGGCCCTCCCGGGGAGGCCACTGTAGTTCCCAGTGCTAACACGGA CCTGGAAGGCCAAGCGGAGGGCAAGAAGGAGGCAGAGGCTGACGATGTGCTGAGAAGAGGCCCCAGGCCC ATCGTTCCCTACAGTTCCATGTTCTGCCTCAGCCCCACCAACCTACTCCGTCGCTTCTGCCATTACATTG TGACCATGCGGTACTTTGAGATGGTGATTCTTGTGGTCATCGCCTTGAGCAGCATTGCCCTGGCTGCTGA GGATCCCGTGCGGACCGACTCATTCCGGAACAATGCTCTGAAGTACATGGACTACATCTTTACAGGAGTC TTCACCTTTGAGATGGTCATAAAGATGATAGACTTGGGCCTGCTGCTGCACCCTGGGGCCTACTTCCGGG ACCTGTGGAACATTCTGGACTTCATTGTTGTCAGTGGAGCCCTGGTGGCATTTGCATTCTCAGGATCCAA AGGGAAAGACATCAATACCATCAAGTCTCTGAGAGTCCTGCGAGTCCTGCGGCCCCTCAAGACCATCAAG CGGCTGCCTAAACTCAAGGCTGTGTTTGACTGTGTGGTGAACTCTCTGAAGAATGTCTTGAACATCCTGA TCGTCTACATGCTCTTCATGTTTATATTTGCCGTCATCGCCGTCCAACTCTTCAAAGGGAAGTTCTTTTA CTGCACTGATGAGTCCAAGGAGCTGGAGCGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAAGAG GTAGAAGCCCAGCCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTGCTCTGGGCCTTGCTGA CTCTGTTTACGGTGTCCACAGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGACGCCACCTATGA GGAGCAGGGGCCAAGCCCCGGGTTTCGGATGGAGCTTTCCATCTTCTATGTGGTCTACTTTGTGGTCTTC CCTTTTTTCTTTGTCAACATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTGA TGTCTGAGTGCAGTCTGGAAAAGAATGAGAGGGCTTGCATTGACTTTGCCATCAGCGCCAAACCCCTGAC ACGGTACATGCCTCAGAACAAGCAGTCGTTCCAGTATAAGACATGGACATTTGTGGTCTCTCCACCCTTT GAGTACTTCATTATGGCCATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTACGATGCCCCTT ATGAGTACGAGCTGATGCTGAAGTGCTTGAACATCGTCTTCACATCCATGTTCTCTCTGGAGTGCATCCT GAAGATCATCGCCTTCGGGGTGTTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACTGTT TTGGGAAGTATTACTGATATTTTAGTAACGGAGATTGCGGAAACGAACAACTTCATCAACTTGAGCTTCC TTCGCCTCTTCCGGGCAGCACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCTTGTTATG GACCTTTGTCCAGTCCTTTAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTAC GCCATCATCGGCATGCAGGTTTTTGGAAACATTGCCCTTGATGATGGCACCAGCATCAACCGACACAACA ACTTCCGGACATTTCTGCAAGCCTTAATGCTGTTGTTCAGGAGTGCCACTGGGGAGGCCTGGCACGAAAT CATGCTGTCTTGCCTGGGCAACCGGGCCTGCGACCCACATGCCAACGCCAGCGAATGCGGGAGCGACTTT GCCTATTTTTATTTTGTCTCCTTCATCTTCCTCTGTTCCTTTCTGATGCTGAACCTCTTTGTTGCTGTGA TCATGGACAATTTCGAATACCTCACGCGGGATTCTTCCATCCTAGGGCCGCACCACCTCGATGAATTCAT TCGCGTCTGGGCTGAATACGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTG AAACACATGTCCCCACCTCTGGGTTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTC GAATGAACATGCCCATATCCAATGAGGACATGACGGTACACTTTACATCCACACTGATGGCCCTCATCCG GACGGCACTGGAGATCAAGCTTGCCCCAGCGGGGACAAAACAGCACCAATGTGATGCTGAGCTGAGGAAG GAGATCTCTTCTGTGTGGGCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTGCCACCCCACAAACCTG ACGAGATGACAGTGGGGAAGGTCTATGCGGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCAC CAGAGATCAGACTCACCAAGCTCCTGGAGGCCTGTCCCAGATGGGTCCTGTTTCCCTGTTCCATCCTCTG AAGGCCACCCTGGAGCAGACACAGCCCGCTGTGCTCCGAGGAGCTCGGGTTTTCCTTCGACAAAAGAGTG CAACTTCCCTCAGCAATGGGGGCGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCCCTGTCCTGGGG CACGCAGAGGACCCAGGACGTACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATC CCTGTGGGGCAGCCAGGAGCACTGGCTGTAGATGTCCAGATGCAGAACATGACATTGAGAGGACCGGATG GGGAGCCCCAGCCTGGCCTGGAGAGCCAAGGCCGAGCGGCCTCTATGCCACGCCTGGCGGCAGAAACACA GCCGGCCCCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCGCATGGGACTCAG CTTTGCAACACAGTCCTGGACCGGCCACCTCCTAGCCAGGTGTCCCATCACCACCACCACCGCTGCCACC GGCGCAGGGACAAGAAGCAGAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACACAGAAGGTGCACC AAGTACTGCTGCAGGATCTGGCCTGCCCCATGGAGAAGGGTCCACAGGCTGCCGGCGGGAGCGTAAGCAA GAGCGAGGCCGGTCCCAGGAGCGGAGGCAGCCCTCCTCCTCTTCTTCAGAGAAGCAGCGCTTCTATTCCT GTGACCGCTTTGGGAGCCGGGAGCCCCCACAACCTAAGCCCTCCCTCAGTAGCCACCCCATATCGCCAAC AGCGGCACTAGAGCCAGGACCCCACCCGCAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACA TCTGGTGCTAGCACGCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCCCTGACCCCACGCCCCA GCATCACCTACAAGACGGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGTGGCCTTCCAGCCTT CTCCCCTGGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAG CCTCTAGCTTCTGGCTCCCGCATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCTG CCCACAACCTGCCTGAGGATACACTCACCTTTGAAGAGGCCGTGGCCACCAACTCTGGCCGCTCCTCCAG GACTTCCTATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTACCACTGC ACTTTGGGACTCAGCACCGGCGTCCGGGCGCGGCACAGCTACCACCACCCAGACCAGGATCACTGGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207906 representing NM_147141 Red=Cloning site Green=Tags(s) MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFT VNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGI FCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQ VVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCDSD TECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNL VLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAA TKKSRNDLIHAEEGEDRFVDLCAAGSPFARASLKSGKTESSSYFRRKEKMFRFLIRRMVKAQSFYWVVLC VVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFE VVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQL FGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTL LNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQ RASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPAGNKSKPEGTE ATEGADPPRRHHRHRDRDKTSASTPAGGEQDRTDCPKAESTETGAREERARPRRSHSKEAPGADTQVRCE RSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGKEGTAPVLVPKGERRARHRGPRTGPRETENSEEPT RRHRAKHKVPPTLEPPEREVAEKESNVVEGDKETRNHQPKEPRCDLEAIAVTGVGSLHMLPSTCLQKVDE QPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGEATVVPSANTDLEGQAEGKKEAEADDVLRRGPRP IVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGV FTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIK RLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEE VEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVF PFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPF EYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSLECILKIIAFGVLNYFRDAWNVFDFVTV LGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIY AIIGMQVFGNIALDDGTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDF AYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEML KHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRK EISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPL KATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDVLYEARAPLERGHSAEI PVGQPGALAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQ LCNTVLDRPPPSQVSHHHHHRCHRRRDKKQRSLEKGPSLSVDTEGAPSTAAGSGLPHGEGSTGCRRERKQ ERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQPKPSLSSHPISPTAALEPGPHPQGSGSVNGSPLMST SGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQ PLASGSRIGSDPYLGQRLDSEASAHNLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHC TLGLSTGVRARHSYHHPDQDHWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_147141
ORF Size	6999 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_147141.1, NP_671482.1
RefSeq Size	9695 bp
RefSeq ORF	7002 bp
Locus ID	257648
MW	261.9 kDa

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