Wnk4 (NM_175579) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ac2-059; Prkwnk4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208361 representing NM_175579 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCACCTCGAAATACAGAGACCGGGGTCCACATGTCCCAAACGGAGGCCGACCTGGCTTTGCGGC CATCTCCGTCTCTCACCTCCATGGGGCCGACCCGCCTGGGGCCACCTCCTCGCCGAGTGCGCCGCTTCTC TGGGAAGGCTGAGCCCCGGCCGCGCTCTTCGAGACCGAGCCGCCGCAGCTCAGTCGATCTGGGACTGTTG AGCTCCTGGTCTCAACCAGCCTCACTCCTTCCGGAACCTCCTGATCCTCCGGACTCCGCTGGTCCCATGA GGAGCCCACCTTCAAACTCTAAAGAACACCCCGAGGGCACATGGACGGGGGCAGCCCCGGTGAAGGCTGC AGACTCTGCCTGTCCTGAGCTTACAGTATCTTCCGGAGGTCCGGGGTCCCGGGAGCCACCAAGGGTTCCT GATGCTGCAGCCCGGGAACGGCGGCGGGAGCAGGAAGAAAAAGAGGACACAGAGACCCAGGCTGTGGCAA CGTCTCCCGACGGCCGATACCTCAAGTTTGACATTGAGATTGGAAGAGGTTCCTTCAAGACCGTGTATCG AGGGCTAGACACCGACACCACGGTAGAGGTGGCCTGGTGTGAGCTGCAGGCTCGGAAACTCTCTCGGGCT GAACGGCAGCGTTTCTCTGAGGAAGTTGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCT ATGACTCCTGGAAGTCGGTGCTGAGGGGTCAGGTTTGCATCGTGCTGGTCACAGAACTCATGACCTCGGG CACGCTCAAGACGTACCTGAGGAGATTCCGAGAAATGAAGCCCCGAGTCCTTCAGCGCTGGAGTCGCCAG ATCCTACGGGGACTTCATTTCTTACATTCCCGAGTGCCCCCCATCCTGCACCGAGATCTCAAATGTGACA ATGTCTTCATTACCGGCCCTTCAGGCTCTGTCAAAATCGGAGATCTCGGACTGGCCACGCTCAAGCGCGC CTCCTTTGCCAAGAGCGTCATTGGGACCCCGGAATTCATGGCCCCTGAGATGTATGAGGAAAAGTACGAC GAGGCTGTGGATGTGTACGCCTTTGGCATGTGCATGCTGGAGATGGCTACGTCTGAGTATCCCTACTCTG AGTGTCAGAACGCAGCACAAATCTACCGAAAGGTCACTTCGGGCACAAAGCCCAACAGCTTCTACAAAGT GAAGATGCCAGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAATGAGAGGTTCACTATC CAGGATCTTCTGACCCACGCGTTCTTCCGCGAGGAACGTGGTGTGCATGTGGAGCTGGCAGAGGAAGATG ATGGAGAGAAGCCGGGTCTCAAACTCTGGCTGCGCATGGAGGACGCACGACGCGGGGGGCGCCCAAGGGA CAACCAGGCCATCGAGTTCCTGTTCCAGCTCGGTCGGGATGCAGCGGAAGAGGTGGCTCAGGAGATGGTA GCCTTGGGCTTAGTCTGCGAAGCAGACTACCAGCCAGTGGCCCGTGCAGTTCGAGAACGGGTTGCTGCCA TCCAGCGGAAGCGGGAGAAGCTAAGAAAAGCCAGGGAATTGGAGGTTCTCCCACCAGACTCAGGACCTCC TCCAGCAACTGTGTCTATGACTCCTGGTCCCCCCAGTGCCTTCCCCCCAGAGCCCGAGGAGCCAGAGGCT GACCAGCACCAATCCTTCCTCTTCCGCCATGCCAGCTACTCATCCACTACATCTGATTGCGAGACTGATG GCTACCTCAGCTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCCGGGGGGATGCCATC CAGCCCCGCTGAGCCCCATCTCTGCTTGCCCTCGGGTTTTGCCTTGTCCATTCCACGGTCTGGCCCTGGC AGTGACTTTTCTCCTGGGGACAGCTACGCCTCAGATGCAGCCTCAGGCCTCAGTGACATGGGAGAAGGGG GGCAAATGAGGAAAAATCCAGTGAAGACTCTGCGACGGAGACCTCGATCCCGGCTTCGGGTCACGAGTGT CTCAGACCAGAGCGACAGAGTCGTGGAGTGCCAGCTGCAAACTCACAACAGCAAGATGGTGACGTTCCGA TTTGATCTGGATGGGGACAGCCCAGAAGAAATCGCAGCTGCCATGGTTTATAATGAATTCATTCTGCCCT CGGAGCGAGACGGATTCCTGAGCCGGATCCGGGAGATTATCCAGCGAGTGGAGACCCTGCTGAAGAGAGA TGCTGGTCCCTCGGAGGCCACTGAAGATGCGCTGAGCCCTCAGGAAGAGGCAGCAGCCATGCCTGCCCTC CCAGGCCCATCCGATGAAGAGCTCCAGAGAAGCATCTCCCCGGAACAGAGGAGCTGGGCAGCCTTCTCTA CATCTCCATCTTCTCCTGGCACCCCCTTGTCCCCTGGAACCCCCTTTTCCCCTGGGACCCCTCCTGTCTT CCCATGCCCCATCTTCCCTATCACTTCACCCTCATGCCATCCCTACCCGTTCTCACAGGTCTCTTCAAAT CCCTGTCCACAGGCCCCCAGCTCCCTACTTCCCTCCTCCTCCGGTGCTTCTCAGGTTCCATTCCCATCCC CTTCCCTTCCCACCAGCTCTCCCCTCCCATTCTCTCCTAGTTATCCCCAAGTCCCTCTTCACCCTGCTTC TCTTCCCACCTGCCCCTCCCCTCCCCCTCTCCCCTCCACCACAGCAGCCCCTCTCCTCTCTCTGGCTAGT GCCTTCTCTCTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCATCCCCTGGGCTTCTCTCTCAGT CTCCTCCAGCCCCTCCAGGTCCTCTGCCTAGCATGCCCCTTCCCCTTGCTTCTTGTGACCAGGAGAGCCT TTCAGCCCAAACAGCTGAGACAGAGAATGAGGCTTCCCGAAATCCTGCTCAGCCACTACTGGGTGATGCT AGACTGGCACCCATATCTGAAGAGGGAAAACCCCAGCTGGTTGGCCGATTCCAAGTGACTTCATCCAAGG AACCAGCAGAGCCTCCCCTACAACCAGCATCTCCAACTCTCTCCAGATCCCTAAAGCTTCCAACCCCTCA GCTGACCTCAGAGAGTTCAGACACAGAGGACAGTGCTGCAGGAGGACCAGAGACCAGGGAGGCTCTGGCA GAGAGTGACCGTGCAGCCGAGGGCTTGGGGGTCGCCATCGATGAGGAAAAGGATGAAGGGAAGGAACCCC AAATTGGGGGCAGTTCCCCAATCTTGAGCCAGCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCT GTGTCTAAGCAGTGAGGAGTCAGAGAGCAGCGGGGAGGACGAGGAGTTCTGGGCTGAGCTGCAGAACCTT CGGCAGAAGCACTTGTCGGAAGTGGAAGCACTACAGACGCTACAGAAGAAGGAAATCGAAGACTTGTACA GCCGGCTTGGAAAGCAACCCCCACCTGGTATTGTAGCTCCAGCGGCTATGCTGTCCTGCCGCCAGCGCCG CCTCTCCAAGGGAAGCTTTCCCACCTCCCGCCGCAACAGCCTGCAGCGCTCTGATCTCCCTGGCCCTGGC ATCATGCGGAAGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGA CATTCGCCGGGGATGTTGGCAGGATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208361 representing NM_175579 Red=Cloning site Green=Tags(s) MLAPRNTETGVHMSQTEADLALRPSPSLTSMGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLL SSWSQPASLLPEPPDPPDSAGPMRSPPSNSKEHPEGTWTGAAPVKAADSACPELTVSSGGPGSREPPRVP DAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQARKLSRA ERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQ ILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD EAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTI QDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMV ALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEA DQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPG SDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFR FDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEAAAMPAL PGPSDEELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSN PCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLAS AFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDA RLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALA ESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNL RQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPG IMRKNSLSGSSTGSQEQRASKGVTFAGDVGRM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175579
ORF Size	3666 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175579.3, NP_783169.2
RefSeq Size	4093 bp
RefSeq ORF	3669 bp
Locus ID	287715
UniProt ID	Q7TPK6
MW	132.8 kDa
Gene Summary	mutation of the human homolog causes pseudohypoaldosteronism type II [RGD, Feb 2006]

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