Srsf6 (NM_001014185) Rat Tagged ORF Clone

CAT#: RR208448

  • TrueORF®

Srsf6 (Myc-DDK-tagged ORF) - Rat splicing factor, arginine/serine-rich 6 (Sfrs6), (10 ug)



  "NM_001014185" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Sfrs6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR208448 representing NM_001014185
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGCGTCTACATAGGACGCCTGAGCTACAACGTCCGCGAGAAGGACATCCAGCGCTTTTTCAGCG
GCTACGGCCGCCTCCTCGAAATCGACCTCAAAAATGGGTACGGTTTCGTGGAGTTCGAGGACTCCCGTGA
TGCGGACGACGCCGTTTACGAGCTGAACAGCAAGGAGCTGTGCGGCGAGCGCGTGATCGTAGAGCACGCC
CGGGGCCCGCGCCGGGACCGCGATGGCTACAGCTACGGAAGCCGCAGTGGTGGAGGTGGATACAGCAGTC
GGAGAACTTCTGGCAGAGACAAATATGGACCACCTGTTCGTACAGAATACAGGCTTATTGTAGAAAATCT
ATCTAGTCGTTGCAGTTGGCAAGACTTAAAGGATTTCATGCGGCAAGCAGGAGAGGTGACTTATGCAGAT
GCTCACAAAGAACGAACAAATGAGGGTGTAATTGAATTTAGATCCTACTCTGACATGAAGCGTGCTTTGG
ATAAACTGGATGGCACAGAAATAAATGGCAGGAATATACGGCTTATTGAAGATAAGCCACGAACAAGCCA
TAGGCGCTCTTACTCTGGAAGTAGATCCAGATCACGGTCTAGAAGAAGATCTCGGAGTAGGAGTCGCAGA
AGCAGCCGCAGTAGATCTCGAAGTATCTCAAAAAGTCGCTCGCGATCTAGGTCTCGGAGCAAAGGTCGAT
CCCGATCCCGCTCAAAGGGCAGGAAATCCAGATCAAAGAGCAAATCTAAGCCCAAGTCTGATCGGGGTTC
CCATTCACATTCAAGAAGCAGATCTAAGGATAAGTATGGGAAGTCACGAAGTAGGTCACGTTCTCGGTCC
CCCAAAGAAAATGGAAAAGGAGATATAAAGTCAAAATCCAGATCCCGGAGCCAGTCTCGATCCCATTCGC
CTCTGCCTGCTCCACCCTCAAAGGCTCGGTCCATGTCCCCTCCGCCAAAAAGAGCTTCAAGGTCCCGTTC
TAGATCTCGTTCCAGGTCCAGATCAAGTTCCAGAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR208448 representing NM_001014185
Red=Cloning site Green=Tags(s)

MPRVYIGRLSYNVREKDIQRFFSGYGRLLEIDLKNGYGFVEFEDSRDADDAVYELNSKELCGERVIVEHA
RGPRRDRDGYSYGSRSGGGGYSSRRTSGRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYAD
AHKERTNEGVIEFRSYSDMKRALDKLDGTEINGRNIRLIEDKPRTSHRRSYSGSRSRSRSRRRSRSRSRR
SSRSRSRSISKSRSRSRSRSKGRSRSRSKGRKSRSKSKSKPKSDRGSHSHSRSRSKDKYGKSRSRSRSRS
PKENGKGDIKSKSRSRSQSRSHSPLPAPPSKARSMSPPPKRASRSRSRSRSRSRSSSRD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014185
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014185.2, NP_001014207.2
RefSeq Size 3513 bp
RefSeq ORF 1020 bp
Locus ID 362264
UniProt ID G3V6S8
MW 39 kDa
Gene Summary Plays a role in constitutive splicing and modulates the selection of alternative splice sites. Plays a role in the alternative splicing of MAPT/Tau exon 10. Binds to alternative exons of TNC pre-mRNA and promotes the expression of alternatively spliced TNC. Plays a role in wound healing and in the regulation of keratinocyte differentiation and proliferation via its role in alternative splicing (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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