Map3k4 (NM_001107456) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208609 representing NM_001107456 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACACCGAGTCAGTCTCCACATAAAGACTTGGGGAAGGGAGTGGAGGCCGTGGAGGAGTACAGCT ACAAGCAGGGGAAGAAGACGCGAGCGACTCTGAGGACAACAGAGCGAGACCATAAGAAAAATGCACAGTG CTCGTTCATGTTGGACTCAGTGGCCGGGTCTTTGCCAAAAAAATCTATTCCAGATGTGGATCTCAATAAG CCTTACCTCAGCCTCGGCTGTAGCAACGCCAAGCTGCCAGTCTCTATGCCCATGCCTATAGCCAGAACTG CGCGGCAGACTTCCCGGACGGACTGCCCAGCGGATCGGTTAAAGTTCTTTGAAACACTGCGCCTTTTGCT AAAGCTTACCTCAGTCTCGAAGAAGAAGGACAGGGAACAGAGGGGACAGGAAAACACGGCTGCTTTCTGG TTCAACCGGTCAAATGAACTGATCTGGTTAGAGCTCCAGGCCTGGCACGCAGGCCGCACCATCAATGACC AGGACCTCTTTTTATACACAGCCCGCCAAGCCATCCCAGATATTATCAATGAAATCCTCACATTCAAAGT TAATTATGGAAGCGCTGCCTTCTCTAGAAATGGAGCTGGTTTCAACGGTACCTCGCTAGAAGGTCAGTGC AGAACCCTTCATGGGACGAACAGTCTAGGCTGTTCAACCTACCACGAGCACCTCCAGCGCCAGAGGGTCT CATTTGAGCAGGTGAAACGGATAATGGAGCTGCTAGAGTACATGGAAGCACTTTATCCGTCCTTGCAGGC TCTGCAGAAGGACTACGAGAGATACGCCGCAAAGGACTTTGAGGACAGAGTGCAGGCACTCTGCCTGTGG CTAAACATCACGAAAGATTTAAATCAGAAGTTGAGGATTATGGGCACTGTTCTGGGCATCAAGAACTTAT CAGACATTGGCTGGCCAGTGTTTGAAATCCCTTCCCCTCGGCCATCCAAAGGCTACGAGCCTGAGGAGGA GGGCGAGGACACAGAGGGAGAGCTGAGGGAGCTGGAGAGTGGGACGGAGGAGAGTGATGAGGAGCGAAGT CCCAATCCGAGGGTGCCAGAGCTCAGGCTGTCTGCCGACACCATCTTGGACAGTCACTCCCAGGGCTGCG CGTCCAGGAAACTTGAGAGGCTCGAGTCCGAGGAGGACTCTGTAGGCTGGGGGATAGCAGACTGTGGTCC CGAAGCCAGTAGGCATTGTTTGACTTCTATCTATAGACCGTTCGTGGACAAAGCACTGAAGCAAATGGGG CTGAGGAAGTTAATTTTACGACTTCATAAGCTAATGAATGGCTCCTTGCAAAGGGCTCGTGTAGCTCTGG TAAAGGATGACCGTCCTGTGGAGTTCTCTGACTTTCCAGGTCCCATGTGGGGCTCGGATTATGTGCAGTT GTCGGGGACGCCTCCTTCCTCGGAGCAGAAGTGCAGTGCTGTGTCCTGGGAAGAGCTGAGGGCCATGGAC CTGCCCTCCTTTGAGCCCGCCTTCCTGGTGCTCTGCCGGGTCCTGCTGAACGTGATCCATGAGTGCCTGA AGCTGCGGCTGGAACAGAGGCCGGCCGGGGAGCCTTCCCTCTTGAGTATCAAGCAGGTGTATTTTGACTA CATGAGAAGCTGGATCCAAATGCTACAGCAGTTACCTCAGGCTTCCCATAGCTTAAAAAACCTGCTAGAA GAAGAATGGAATTTCACCAAAGAAATAACCCATTACATCCGGGGAGGAGAAGCACAGGCTGGAAAGCTTT TCTGTGACATTGCAGGGATGCTGCTGAAATCCACGGGGAGCTTTCTGGACTCTGGCCTACAGGAGAGCTG TGCTGAACTGTGGATCAGCGCCGACGACAACGGTGCTGCCGACGAGCTAAGGAGGTCTGTCATCGAGATC AGCCGAGCACTCAAGGAGCTCTTCCATGAGGCCCGGGAAAGAGCCTCCAAGGCCCTGGGCTTTGCTAAAA TGCTGAGGAAGGACCTAGAAATAGCAGCAGAGTTTGTTCTGTCTGCGTCTGCCCGAGAGCTCCTAGACGT TCTGAGAGCAAAGCAGTATGTTAAGGTACAGATTCCTGGGCTAGAGAATTTGCACGTGTTTGTCCCCGAC AGCCTCGCTGAGGAGAAGAAAATTATTTTGCAGCTACTCAATGCTGCCACAGGAAAGGACTGCTCAAAGG ATCCGGACGACGTCTTTGTGGATGCCTTCCTGCTCCTGACCAAGCATGGGGACCGAGCCCGTGACTCAGA AGATGGCTGGAGCACATGGGAAGCACGAGCTATCAAAATTGTGCCTCAGGTGGAGACCGTGGACACCCTG AGAAGCATGCAGGTGGACAACCTTCTGCTGGTTGTCATGGAGTCTGCTCACCTCGTACTTCAGAGAAAGG CCTTCCAGCAGTCCATTGAGGGGCTGATGACTGTACACCATGAGCAGACATCCAGCCAGCCAGTCATTGC CAAAGCTTTACAGCAGCTAAAGAACGATGCGCTCGAGCTGTGCAACAGAATAAGCGATGCCATCGACCGC GTGGACCACATGTTCACGCTGGAGTTGGATGCCGAGGTCGAGGAGTCCGAGTCGGCCACGCTGCAGCAGT ACTACCGAGAGGCCATGATTCAGGGCTACAACTTCGGGTTTGAGTATCATAAAGAAGTTGTTCGTTTGAT GTCTGGAGAATTCAGACAGAAGATAGGAGACAAATATATAAGCTTTGCCCAGAAGTGGATGAATTATGTG CTGACCAAATGTGAGAGCGGCAGAGGCACAAGACCCAGATGGGCCACCCAAGGTTTTGATTTCCTACAAG CCATTGAACCTGCCTTTATTTCAGCTTTACCAGAAGATGACTTCTTGAGTTTGCAAGCCCTGATGAATGA GTGCATTGGGCACGTCATAGGAAAGCCACACAGCCCTGTCACAGCTATCCATCGGAACAGCCCCCGCCCT GTGAAGGTGCCCCGATGCCACAGTGACCCTCCTAACCCTCACCTCATCATCCCAACTCCAGAGGGATTCA GGGGTTCCAGCGTCCCTGAAAATGACCGCTTGGCCTCCATAGCTGCAGAACTGCAGTTCAGGTCTCTGAG TCGGCACTCGAGCCCCACGGAAGAGCGAGACGAGCCAGCATATCCTCGGAGCGACTCAAGTGGATCAACA CGGAGAAGCTGGGAACTTCGAACACTCATCAGCCAGACCAAAGACTCGGCCTCTAAGCAGGGGCCCATAG AGGCCATCCAGAAGTCAGTCCGACTGTTTGAAGAGAGGAGGTATCGAGAGATGAGGAGGAAGAATATCAT CGGTCAAGTGTGCGATACCCCTAAGTCCTATGATAACATCATGCATGTTGGACTGAGGAAGGTGACGTTT AAGTGGCAAAGAGGAAACAAAATCGGAGAAGGGCAGTATGGAAAAGTGTACACCTGCATCAGCGTCGACA CAGGGGAGCTGATGGCCATGAAGGAGATTCGATTTCAGCCTAACGACCATAAGACTATCAAGGAAACTGC AGACGAGTTGAAAATATTTGAAGGCATCAAGCACCCCAACCTGGTCCGGTACTTTGGTGTGGAGCTTCAC AGGGAAGAGATGTACATCTTCATGGAGTACTGTGATGAAGGCACACTAGAGGAGGTGTCAAGGCTGGGCC TTCAGGAGCACGTCATCCGGTTGTATTCCAAGCAGATCACTGTCGCCATCAACGTCCTCCATGAGCATGG CATCGTTCACCGAGACATCAAAGGTGCCAATATCTTCCTTACATCGTCTGGACTAATCAAACTGGGAGAT TTTGGATGCTCAGTGAAACTTAAAAACAATGCTCAGACCATGCCTGGCGAGGTGAACAGCACCCTAGGGA CAGCAGCTTACATGGCCCCTGAAGTTATTACCCGAGCTAAAGGAGAAGGCCACGGACGTGCGGCAGACAT CTGGAGTCTGGGGTGCGTCGTCATAGAGATGGTGACTGGCAAGCGGCCTTGGCATGAGTTTGAACACAAC TTTCAGATTATGTACAAGGTGGGAATGGGACACAAGCCACCAATCCCCGAAAGGTTAAGCCCTGAAGGAA AGGACTTTCTCTCGCACTGCCTGGAAAGTGACCCGAAGATAAGGTGGACAGCCAGCCAGCTCCTTGACCA CGCTTTTGTCAAGGTTTGCACAGATGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208609 representing NM_001107456 Red=Cloning site Green=Tags(s) MNTPSQSPHKDLGKGVEAVEEYSYKQGKKTRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNK PYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFW FNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSAAFSRNGAGFNGTSLEGQC RTLHGTNSLGCSTYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLW LNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEEEGEDTEGELRELESGTEESDEERS PNPRVPELRLSADTILDSHSQGCASRKLERLESEEDSVGWGIADCGPEASRHCLTSIYRPFVDKALKQMG LRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMD LPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQVYFDYMRSWIQMLQQLPQASHSLKNLLE EEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLDSGLQESCAELWISADDNGAADELRRSVIEI SRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDVLRAKQYVKVQIPGLENLHVFVPD SLAEEKKIILQLLNAATGKDCSKDPDDVFVDAFLLLTKHGDRARDSEDGWSTWEARAIKIVPQVETVDTL RSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVHHEQTSSQPVIAKALQQLKNDALELCNRISDAIDR VDHMFTLELDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYV LTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRP VKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGST RRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNIMHVGLRKVTF KWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELH REEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGD FGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEFEHN FQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107456
ORF Size	4161 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107456.1, NP_001100926.1
RefSeq Size	4908 bp
RefSeq ORF	4164 bp
Locus ID	308106
MW	157.3 kDa

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