Taok2 (NM_022702) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Tao2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208910 representing NM_022702 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCTGATGTAGCTGAGCTCTTCTTCAAAGATGACC CTGAGAAGCTTTTCTCTGACCTCCGGGAAATTGGCCATGGCAGTTTTGGAGCTGTGTACTTTGCCCGGGA TGTCCGGAACAGTGAGGTGGTGGCCATCAAGAAGATGTCCTATAGTGGGAAGCAATCAAATGAGAAATGG CAGGATATCATCAAGGAGGTGCGGTTCTTACAGAAGCTACGGCATCCTAATACCATTCAGTACCGGGGCT GTTACCTGAGGGAGCACACAGCTTGGCTGGTGATGGAGTATTGCCTGGGTTCAGCTTCTGATCTTCTCGA AGTGCACAAGAAGCCGCTGCAGGAGGTAGAGATTGCAGCTGTGACCCATGGTGCGCTTCAGGGCCTGGCC TATCTACATTCACACAACATGATCCATAGAGATGTGAAGGCTGGGAACATCTTGCTGTCAGAACCAGGCT TGGTGAAACTGGGGGACTTTGGCTCCGCATCCATCATGGCACCTGCCAACTCATTTGTGGGCACTCCATA CTGGATGGCTCCAGAGGTGATCCTAGCCATGGATGAGGGACAATATGATGGCAAAGTGGATGTCTGGTCC TTGGGGATAACCTGTATTGAGCTAGCGGAGCGGAAGCCACCACTGTTTAACATGAATGCAATGAGTGCCT TATACCACATTGCACAGAATGAATCCCCTGCTCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT TGTTGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTTTTGAAGCACCGCTTT GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTAATTCAGAGGACCAAGGATGCTGTACGGGAAC TAGATAACCTGCAGTACCGAAAGATGAAGAAGATACTATTCCAAGAGGCACCCAATGGCCCTGGTGCTGA GGCCCCAGAGGAAGAGGAGGAAGCAGAACCTTACATGCACCGAGCAGGGACACTGACCAGTCTAGAGAGT AGCCATTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAAAGCAGCTCAGTCAACAGCCTAGCAGATG CCTCAGATAATGAAGAAGAGGAGGAGGAGGAAGAGGAAGAAGAAGAGGAGGAGGAAGAAGAAGGCCCTGA ATCCCGAGAGATGGCCATGATGCAGGAGGGGGAGCATACAGTCACTTCCCACAGCTCCATCATCCACCGG CTGCCGGGCTCAGACAACCTATATGATGATCCCTACCAGCCAGAGATGACCCCAGGTCCACTCCAACCAC CTGCAGCCCCTCCCACCTCCACCTCCTCCTCTTCTGCTCGCCGCAGAGCTTATTGCCGCAACCGAGACCA CTTTGCCACCATCCGTACTGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCGGCCCTG CGGGAGCAACTAAGTGGCTACAAGCGGATGCGGCGTCAGCACCAGAAGCAACTGCTGGCCCTGGAGTCCC GTCTGAGGGGTGAACGTGAGGAGCACAGTGGGCGGTTGCAGCGTGAACTCGAGGCACAGCGGGCTGGCTT TGGGACTGAGGCTGAGAAGCTGGCCCGGAGGCACCAGGCCATTGGTGAGAAGGAAGCACGAGCTGCTCAG GCTGAGGAGCGGAAGTTCCAGCAGCACATCTTGGGGCAGCAGAAGAAGGAACTGGCTGCCCTGCTGGAGG CACAGAAGCGAACCTATAAGCTTCGGAAGGAGCAGTTGAAAGAGGAGCTCCAGGAGAACCCTAGCACACC CAAACGAGAGAAGGCTGAGTGGCTGTTGAGGCAGAAAGAGCAGTTGCAACAGTGCCAGGCAGAGGAGGAG GCAGGGCTACTGCGGAGGCAACGCCAGTACTTTGAGCTTCAGTGTCGCCAATACAAGCGCAAGATGCTAC TGGCTCGGCACAGCCTAGACCAGGACCTGCTTCGAGAGGACTTGAATAAGAAACAGACACAGAAGGACTT GGAGTGTGCTCTGCTGTTACGGCAGCATGAGGCTACCCGAGAGCTGGAGCTACGACAGCTCCAGGCTGTC CAGCGCACACGTGCTGAACTCACCCGCCTTCAGCACCAGACAGAGCTAGGCAACCAGTTGGAGTACAACA AGCGACGGGAGCAAGAGTTGCGGCAGAAGCACGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAAGT ACGTGCAGGCCAGCTACCCATGGGCCTCCCTGCTACCGGGGCTCTGGGACCACTCAGCACAGGCACCCTT AGTGAAGAGCAGCCCTGCTCATCTGGCCAGGAGGCAATCCTGGGCCAAAGGATGCTGGGAGAGGAGGAGG AAGCAGTGCCAGAGAGAATGATTCTGGGAAAGGAAGGGACTACTTTGGAGCCAGAGGAGCAGAGGATTCT GGGGGAAGAAATGGGAACCTTTAGTTCCAGCCCACAAAAACATAGGAGTCTGGTTAATGAGGAAGATTGG GATATATCTAAAGAAATGAAGGAGAGTAGAGTCCCATCCCTGGCATCCCAGGAGAGAAATATTATTGGCC AGGAAGAGGCTGGGGCATGGAATCTGTGGGAGAAGGAGCATGGAAACCTTGTGGATATGGAGTTCAAGCT TGGCTGGGTCCAGGGTCCAGTTCTGACTCCAGTGCCTGAGGAGGAAGAGGAGGAGGAAGAGGAGGGAGGG GCTCCAATTGGAACCCCCAGGGACCCTGGAGATGGCTGTCCTTCCCCAGATATCCCCCCAGAGCCACCTC CATCACATCTGAGACAGTACCCTGCTAGCCAGCTTCCTGGATTCTTGTCTCATGGCCTCCTGACTGGCCT CTCCTTTGCAGTGGGGTCCTCCTCTGGCCTCTTGCCCCTACTACTTCTGCTGCTACTCCCATTGCTGGCA GCCCAGGGTGGAGGTGGCTTGCAGGCAGCACTGCTGGCCCTTGAGGTAGGACTAGTGGGCCTGGGGGCCT CCTACCTGTTCCTTTGTACAGCTCTACACCTGCCACCCAGTCTGTTCTTACTCCTGGCTCAGGGCACTGC ACTGGGGGCTGTCCTTAGCCTGAGCTGGCGCAGAGGCCTTATGGGTGTGCCTCTGGGCCTTGGGGCTGCC TGGCTCCTAGCTTGGCCCAGCCTGGCTTTACCTCTGGCAGCTATGGCGGCTGGGGGCAAATGGGTACGGC AGCAAGGCCCCCAGATGCGTCGGGGCATCTCTCGACTCTGGTTGCGGGTTCTGCTACGCCTGTCACCCAT GGTCTTTCGGGCCCTACAGGGCTGTGCGGCTGTGGGAGACCGGGGGCTGTTTGCCCTGTACCCTAAGACC AATAAGAATGGTTTCCGAAGTCGACTGCCTGTCCCTTGGCCCCGTCAGGGAAATCCTCGCACTACACAGC ACCCACTAGCTCTGTTAGCAAGAGTTTGGGCTCTGTGCAAGGGCTGGAACTGGCGCCTAGCACGGGCTAG CCATAGATTAGCTTCTTGTTTGCCCCCCTGGGCTGTTCATATACTAGCTAGCTGGGGCCTGCTTAAGGGT GAAAGGCCCAGTCGGATCCCTCGGCTGCTACCGCGAAGCCAACGCCGTCTTGGGCTCTCAGCTTCCCGAC AGCTACCACCAGGGACTGTAGCTGGGCGGAGATCTCAGACCCGCAGGGCCCTGCCTCCCTGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208910 representing NM_022702 Red=Cloning site Green=Tags(s) MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKW QDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLA YLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS LGITCIELAERKPPLFNMNAMSALYHIAQNESPALQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRF VLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLES SHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEEEGPESREMAMMQEGEHTVTSHSSIIHR LPGSDNLYDDPYQPEMTPGPLQPPAAPPTSTSSSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSAL REQLSGYKRMRRQHQKQLLALESRLRGEREEHSGRLQRELEAQRAGFGTEAEKLARRHQAIGEKEARAAQ AEERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEE AGLLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAV QRTRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKVRAGQLPMGLPATGALGPLSTGTL SEEQPCSSGQEAILGQRMLGEEEEAVPERMILGKEGTTLEPEEQRILGEEMGTFSSSPQKHRSLVNEEDW DISKEMKESRVPSLASQERNIIGQEEAGAWNLWEKEHGNLVDMEFKLGWVQGPVLTPVPEEEEEEEEEGG APIGTPRDPGDGCPSPDIPPEPPPSHLRQYPASQLPGFLSHGLLTGLSFAVGSSSGLLPLLLLLLLPLLA AQGGGGLQAALLALEVGLVGLGASYLFLCTALHLPPSLFLLLAQGTALGAVLSLSWRRGLMGVPLGLGAA WLLAWPSLALPLAAMAAGGKWVRQQGPQMRRGISRLWLRVLLRLSPMVFRALQGCAAVGDRGLFALYPKT NKNGFRSRLPVPWPRQGNPRTTQHPLALLARVWALCKGWNWRLARASHRLASCLPPWAVHILASWGLLKG ERPSRIPRLLPRSQRRLGLSASRQLPPGTVAGRRSQTRRALPPWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022702
ORF Size	3705 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022702.1, NP_073193.1
RefSeq Size	4298 bp
RefSeq ORF	3708 bp
Locus ID	64666
UniProt ID	Q9JLS3
MW	138.8 kDa
Gene Summary	present in stress-sensitive kinase cascades; selectively activates MEK 3,4, and 6 of the stress-responsive mitogen-activated protein kinase pathways [RGD, Feb 2006]

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