Map3k6 (NM_001107909) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209109 representing NM_001107909 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCCGTGTCCCGGGGCCGGGGTTCTAGAACGGGCGGGCAGCTGCTGGCAGGACCCTCTGGCGG AGGCGCTGAGCCGGGGCCGCTCGTCTCCTGCGGTCGCCGGTCGGGGCTGCGCGCGGAGCCGGCCTCTCAG TGTGGTCTACGTGTTGACCCGCGAGCCTGGACCCGGGGTGGAGCCCGGGTCGGGAACCGAGGCAGAGCCG CTGCCGCTGCGATGCCTGCGCGAGGCCTGTGCTCAGCTCCAGAGCACGCGGCCACCCCCGCAACTGCGCA GCCTGCCCTTTGCGACGCTGGCGCTAGGCGACACCGCTGCGCTAGACTCCTTCTACAACGCGGACGTAGT GGTGCTGGAGGTGAGCAACTCCCTGGCACAGCCTTCCTTGTTCTACCACCTCGGCGTGCGTGAGAGCTTC AGCATGACCAACAATGTGCTCCTCTGTTCCCAGGCAGAGCTCCCTGACCTGCAGGCCCTTCGTGAGGATG TTTTCCAGAAGAACTCGGATTGCGTTGGCAGCTACACACTGATTCCTTATGTTGTGACAGCCACTGGCCG GGTCTTATGTGGCGATGCAGGCCTCCTGAGGGGCATAGCCGATGGGCTAGTACAGGCTGGGGTGGGCACT GAGGCCCTCCTCACGCCCCTGGTGGGCCGGCTTGTCCGTCTCTTGGAGGCCACACCCACAGACTCTTGCG GCTACTTCCGGGAGACCATTCGTCAAGATATCCGGAAGGCACGGGAGCGGTTCAGCGGGCAGCAGCTGAG GCAGGAGCTGGCTCGCCTGCAGCGGAGGCTGGACAGCGTAGAGCTGTTGAGCCCCGACATCATCATGAAT CTGCTTCTGTCATACCGCGACGTCCAGGACTACTCCGCCATCATTGAGCTGGTGGAGACGCTGCAGGCTT TGCCCACCTGTGATGTGGCCGAACAGCACAATGTCTGCTTCCACTACACGTTTGCCCTCAACAGGAGGAA CAGGCCTGGGGACCGTGAGAAGGCCCTGGCTGTCCTTCTGCCATTGGTGAAGCATGAAGGCCCCGTGGCT CCAGACCTGTACTGCATGTGTGGTCGTATCTACAAGGACATGTTCTTCAGCTCCAGCTTCCAAAGTACTG GGCACCTGGAGCAGGCCTATCACTGGTACCGCAAGGCATTTGATGTGGAGCCCAGCCTACACTCGGGCAT CAATGCAGCTGTGCTGCTCATTGCAGCTGGGAAACACTTTGACGACTCTGAGGAGCTCCAGCTGATAGGC ATGAAGCTGGGCTGCCTGTTAGCCCGCAAAGGCTGTGTGGAGAAGATGCAGTATTACTGGGACGTGGGGT TCTACCTGGGAGCCCAGATTCTTGCCAACGACCCCATCCAGGTGGTGCTGGCTGCAGAACAACTATACAA GCTCAATGCCCCCATATGGTACTTGGTGTCAGTGATGGAGACCTTCTTACTGTACCAGCATTTCAGACCC ACACCAGAGCCCTCGGGAGGACCCGTGCTGCGAGCTCACTTTTGGCTCCATTTCTTGCTACAATCCTGCC AGCCTTTCAAGGCGACCCCTCCTCAGGAGGACCAGTGCCTGGTACTGGTGCTGGAGATAAACAAGGTTCT GCTGCCTGCCAGACTTGAGATTCAGGGAGCCGACCCCATGAGCGCAGTGACCCTAAGCTTGCTGGAGCCA GAGACTCAGGATGATCCTTCCAGCTGGACCTTCCCAGTCACCTCCATCTGTGGGATCAGCGCCTCCAAGC TGGACCAGCGCTGCTGCTTCCTGTACGCACTTCCTCCGGCCCAGGACGTCCAGCTGTGCTTTCCCAGCGT AGAGCGCTGCCGGTGGTTCTGTAGCCAAATTCAGGTCTTGGTGATGAATCCAGATTCCTCGGCGCCCACT GAGGAGGCAGAAGGCACAAGGGAGGTGCTGGAGTTTGATTATGAATATTCGGAGACGGGTGAGCGGCTGG TGCTGGGCAAAGGCACGTACGGGGTGGTGTATGCTGGCCGCGACAGGCACACGAGGGTACGAATCGCCAT CAAAGAGATCCCAGAGAGAGATAGCAGATTCTCTCAGCCTCTGCACGAGGAGATCGCTCTCCACAAACGC CTTCGCCACAAGAATATAGTGCGCTATTTGGGCTCAGCCAGCCAGGGCGGCTACCTCAAGATCTTCATGG AGGAAGTGCCTGGAGGCAGCCTGTCCTCCTTGCTTAGGTCAGTGTGGGGACCCCTAAAGGACAACGAGAG TACTATTAGCTTCTACACGCGTCAGATCCTGCAAGGACTTAGCTACCTGCATGAGAACCGCATTGTTCAC CGAGACATCAAGGGGGACAATGTACTGATCAACACCTTCAGTGGGCTGCTCAAGATTTCTGACTTTGGCA CCTCCAAGCGGCTGGCAGGCATCACACCCTGCACGGAGACTTTCACAGGGACCCTGCAGTATATGGCCCC AGAAATCATTGACCAGGGCCCACGAGGATATGGAAAGGCGGCTGACATCTGGTCTCTGGGCTGCACTGTG ATTGAGATGGCCACAGGTTGGCCGCCCTTTCACGAACTAGGGAGCCCGCAGGCTGCCATGTTTCAGGTGG GCATGTACAAGGTACATCCACCAGTGCCAAGTTCCCTGTCAGCTGAGGCCCAAGCCTTCCTCCTCCGAAC TTTTGAGCCAGATCCCCGCCTCCGAGCCAGTGCCCAAGAGCTGTTGGGAGACCCCTTCCTGCAACCAGGG AAGAGGAGCCGCAGCCCTGGCTCTCCTCGGCACACTCCCCGGCCCTCAGGCACCCCTTCCAGTACTTCCA GTCCTTCAGCTGACTCAGCCACACAGTCTCAGACATTCCCAAGGCCCCAGGCACCCTCTCAGCACCCACC CAGCCCTCCGAAGCGCTGCCTTAGTTATGGGGACACCAGTCAGCTCCGTGTGCCCGAGGAATCCGCTGCC GAGGAACCCGCGTCCCCAGAAGAGAGTTCAGGCCTGAGCCTGCTGCACCAGGAGAGCAAGCGCCGAGCCA TGCTGGCTGCAGTGCTGGAACAGGAGGTGCCCATACTAGCAGAGAATCTCCTGGATCAGGAACAGGATTC TCGACTCAGCAGGAATCATGTGGAACTTCTGCTTCGGTGCCTCGGGGCACAAATCCATACTCCTAACCGC CGGCAGCTGGCCCAGGAGCTGCGGGCCCTGCAAGCTCAACTGCGGGCCCAGAGCCTGAGCCCTGCGCTTT TGAAGGGGCCGCTCTTCGCCTTTCCAGACGCGGTGAAGCAGATCCTCCGCAGACGCCAGATCCGCCCACA CTGGATGTTCGTGTTGGACTCGCTGCTCAGCCGGGCAGTCCGGGCGGCGCTGGCCGTACTGGACGCGGAG GCAGAGAAGAAAGCGGTCTCACCGAAGTCAGAGGAGTGGAGTCAAGGGGAGTCCCAGCAGAAACCGCTGG AGAGCCAGCCGCCGCTGCAGAGTCAGCTCCCACCAGAGCAGGAACCTCCATCCCTGATGATACAGCTGGG TCTCTTGCGAGCTGAGACTGACAGGCTTCGGGACATTCTGGCTGAGAAGGAACGTGAGTGCCAGGCCCTC GTGCAACAGGCCCTGCATCGGGTGCACGCAGAGACCAGGACGTATGCCCTGGCTTCAGAGACCCCGGCCA CTCTCCCAAAGGACCAGAAACTGGTGCAGTGGCTACAGGACCTGAGTGTAGATCCAACCACTATCCAAAC GCTCCTGAGCCATAGCTTTACCCTTCAAACCCTGCTCACCTGTGCCACTCAGGATGACCTCGTCTACACC AGAATCAGGGGAGGGATGGTATGCAGAATTTGGAGAGCCATCTTGGCACAGCGAGCAGGAGCCACGTCGG TCACCCCTGGACCCCGGGATGCTGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209109 representing NM_001107909 Red=Cloning site Green=Tags(s) MAGPCPGAGVLERAGSCWQDPLAEALSRGRSSPAVAGRGCARSRPLSVVYVLTREPGPGVEPGSGTEAEP LPLRCLREACAQLQSTRPPPQLRSLPFATLALGDTAALDSFYNADVVVLEVSNSLAQPSLFYHLGVRESF SMTNNVLLCSQAELPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGIADGLVQAGVGT EALLTPLVGRLVRLLEATPTDSCGYFRETIRQDIRKARERFSGQQLRQELARLQRRLDSVELLSPDIIMN LLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDREKALAVLLPLVKHEGPVA PDLYCMCGRIYKDMFFSSSFQSTGHLEQAYHWYRKAFDVEPSLHSGINAAVLLIAAGKHFDDSEELQLIG MKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDPIQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRP TPEPSGGPVLRAHFWLHFLLQSCQPFKATPPQEDQCLVLVLEINKVLLPARLEIQGADPMSAVTLSLLEP ETQDDPSSWTFPVTSICGISASKLDQRCCFLYALPPAQDVQLCFPSVERCRWFCSQIQVLVMNPDSSAPT EEAEGTREVLEFDYEYSETGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHKR LRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLSYLHENRIVH RDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTV IEMATGWPPFHELGSPQAAMFQVGMYKVHPPVPSSLSAEAQAFLLRTFEPDPRLRASAQELLGDPFLQPG KRSRSPGSPRHTPRPSGTPSSTSSPSADSATQSQTFPRPQAPSQHPPSPPKRCLSYGDTSQLRVPEESAA EEPASPEESSGLSLLHQESKRRAMLAAVLEQEVPILAENLLDQEQDSRLSRNHVELLLRCLGAQIHTPNR RQLAQELRALQAQLRAQSLSPALLKGPLFAFPDAVKQILRRRQIRPHWMFVLDSLLSRAVRAALAVLDAE AEKKAVSPKSEEWSQGESQQKPLESQPPLQSQLPPEQEPPSLMIQLGLLRAETDRLRDILAEKERECQAL VQQALHRVHAETRTYALASETPATLPKDQKLVQWLQDLSVDPTTIQTLLSHSFTLQTLLTCATQDDLVYT RIRGGMVCRIWRAILAQRAGATSVTPGPRDAE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107909
ORF Size	3876 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107909.1, NP_001101379.1
RefSeq Size	4339 bp
RefSeq ORF	3879 bp
Locus ID	313022
MW	143.2 kDa

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