Siah2 (NM_134457) Rat Tagged ORF Clone
CAT#: RR209142
- TrueORF®
Siah2 (Myc-DDK-tagged ORF) - Rat seven in absentia 2 (Siah2), (10 ug)
"NM_134457" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209142 representing NM_134457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCCGTCCTCCACCGGCCCCAGCGCTAACAAACCCTGCAGCAAGCAGCCGCCGCCGCCTCAGA CCCCGCACGCCCCGTCCCCGGCTGCGCCCCCGGCTGCCGCCACCATCTCGGCTGCGGGCCCCGGCTCGTC CGCGGTGCCCGCCGCGGCAGCGGTGATCTCAGGCCCCGGCGCCGGCGGCGGGGCCGGCCCAGTGTCCCCG CAGCACCACGAGCTGACCTCGCTGTTCGAGTGCCCGGTCTGTTTTGACTATGTCCTGCCCCCCATTCTGC AGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTGAGCTGCTGCCCGACGTGCAGGGG CGCCCTAACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTCGCCTCGGCAGTTCTGTTTCCCTGTAAG TATGCCACCACGGGCTGTTCCCTGACTCTACACCATACAGAGAAACCAGAGCATGAAGACATCTGTGAAT ACCGTCCTTATTCCTGTCCCTGTCCTGGTGCCTCCTGCAAGTGGCAAGGATCCCTAGAAGCTGTGATGTC CCATCTCATGCATGCCCACAAGAGCATCACTACCCTTCAGGGAGAGGACATAGTCTTTCTAGCTACAGAC ATTAACCTGCCAGGGGCTGTGGACTGGGTGATGATGCAGTCCTGTTTTGGCCACCACTTCATGCTGGTAC TCGAGAAGCAAGAGAAGTACGAAGGTCACCAGCAGTTCTTTGCCATCGTCCTCCTCATTGGCACCCGCAA GCAAGCAGAGAACTTTGCCTACAGACTGGAGTTGAATGGGAACCGGAGGAGACTGACCTGGGAGGCCACA CCCCGGTCCATTCATGATGGTGTGGCTGCAGCCATCATGAACAGTGACTGCCTTGTTTTTGACACAGCCA TAGCACATCTCTTTGCAGATAATGGGAACCTTGGAATCAATGTCACAATATCTACATGCTGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209142 representing NM_134457
Red=Cloning site Green=Tags(s) MSRPSSTGPSANKPCSKQPPPPQTPHAPSPAAPPAAATISAAGPGSSAVPAAAAVISGPGAGGGAGPVSP QHHELTSLFECPVCFDYVLPPILQCQAGHLVCNQCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCK YATTGCSLTLHHTEKPEHEDICEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATD INLPGAVDWVMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTWEAT PRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134457 |
ORF Size | 975 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134457.1, NP_604452.1 |
RefSeq Size | 2048 bp |
RefSeq ORF | 978 bp |
Locus ID | 140593 |
UniProt ID | Q8R4T2 |
MW | 34.7 kDa |
Gene Summary | a synaptophysin binding protein and also interact with E2 ubiquitin-conjugating enzyme UbcH8; involved in the regulating the ubiquitination and degradation of synaptophysin [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209142 | Siah2 (untagged ORF) - Rat seven in absentia 2 (Siah2), (10 ug) |
CNY 3,990.00 |
|
RR209142L3 | Lenti ORF clone of Siah2 (Myc-DDK-tagged ORF) - Rat seven in absentia 2 (Siah2), (10 ug) |
CNY 6,080.00 |
|
RR209142L4 | Lenti ORF clone of Siah2 (mGFP-tagged ORF) - Rat seven in absentia 2 (Siah2), (10 ug) |
CNY 6,650.00 |