Pygm (NM_012638) Rat Tagged ORF Clone
CAT#: RR209227
- TrueORF®
Pygm (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, muscle (Pygm), (10 ug)
"NM_012638" in other vectors (3)
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CNY 10,740.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Muscpho |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209227 representing NM_012638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAATCAGTGTTCGTGGCCTAGCTGGCGTGGAAA ATGTGTCTGACCTGAAAAAGAACTTCAACCGCCACCTACATTTCACCCTGGTCAAGGATCGCAATGTGGC CACTCCGAGAGATTACTATTTCGCACTGGCTCACACCGTGCGGGACCACCTCGTGGGACGCTGGATCCGC ACACAGCAGCATTACTACGAAAAGGACCCCAAGAGGATCTACTACCTGTCTTTGGAGTTCTACATGGGGA GGACACTGCAGAACACCATGGTGAACCTGGCCTTGGAGAATGCCTGCGATGAGGCCACCTACCAGCTGGG TCTGGACATGGAGGAGCTGGAGGAGATCGAGGAGGATGCAGGGTTGGGCAATGGAGGCCTGGGACGCCTG GCAGCTTGCTTCCTGGACTCCATGGCTACACTGGGGCTAGCTGCCTATGGCTACGGGATCCGCTATGAGT TTGGGATTTTTAATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTGATGACTGGCTTCGCTATGG CAACCCCTGGGAGAAGGCCCGCCCAGAGTTCACGCTGCCTGTGCACTTCTACGGTCGAGTGGAGCACACC AGTCAGGGTGCCAAGTGGGTGGACACACAGGTAGTGTTGGCTATGCCCTATGACACTCCTGTGCCTGGCT ATCGAAACAACGTTGTCAACACCATGCGCCTCTGGTCGGCCAAGGCACCTAATGACTTCAACCTCAAGGA CTTCAACGTTGGCGGCTACATCCAGGCCGTGCTGGACCGAAACCTGGCTGAGAACATCTCACGTGTTTTG TACCCCAATGATAACTTCTTTGAAGGGAAGGAATTGCGGCTGAAGCAGGAGTACTTTGTGGTGGCCGCCA CCCTCCAGGACATCATTCGCCGTTTCAAGTCCTCCAAGTTTGGCTGCCGTGATCCTGTGCGCACAAACTT TGATGCTTTCCCTGATAAGGTGGCCATCCAGCTCAACGACACCCACCCTTCTTTGGCCATCCCTGAACTG ATGAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAAGGCCTGGGATGTGACAGTGAAGACCTGTG CCTACACCAACCACACAGTGCTGCCTGAAGCCCTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCT TCCCCGCCATCTGCAGATCATTTATGAGATCAACCAGCGTTTCCTTAATCGGGTGGCGGCCGCATTCCCT GGGGACGTAGATAGGCTGCGGCGCATGTCACTCGTGGAGGAAGGAGCGGTGAAACGCATCAACATGGCAC ACTTGTGCATTGCTGGTTCCCACGCAGTCAACGGTGTGGCTCGCATCCATTCGGAGATCCTCAAGAAAAC CATCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCCAGAACAAGACCAATGGCATCACCCCTCGG CGCTGGTTGGTTCTGTGTAACCCTGGGCTGGCAGAGGTCATTGCTGAGCGCATCGGTGAGGAGTACATCT CGGATCTGGACCAGCTACGCAAGCTGCTCTCCTATGTGGATGATGAAGCCTTTATCCGTGATGTGGCCAA AGTGAAACAGGAAAACAAGTTGAAATTCTCTGCGTACCTGGAGAGGGAATACAAAGTCCACATCAACCCC AACTCCCTCTTCGACGTCCAGGTGAAGAGGATTCATGAGTATAAACGCCAGCTTCTCAATTGCCTTCACA TCATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGGTTCATGGTGCCAAGGACTATCATGATTGG AGGCAAGGCTGCACCCGGGTACCACATGGCTAAGATGATCATCAAGCTTATCACCGCCATTGGGGATGTG GTCAACCACGACCCTGCGGTGGGAGACCGCCTCCGAGTGATCTTCCTGGAGAACTACCGAGTCTCACTGG CTGAGAAAGTTATTCCCGCTGCTGACCTCTCAGAGCAGATCTCCACCGCGGGCACTGAGGCCTCAGGCAC TGGCAACATGAAGTTCATGCTCAACGGGGCTCTGACCATCGGCACCATGGATGGTGCCAACGTGGAGATG GCAGAGGAGGCTGGGGAAGATAACTTCTTCATCTTCGGCATGCGGGTGGAGGACGTGGAAAGGCTGGACC AGAGAGGGTACAATGCCCAGGAATACTACGACCGGATTCCTGAGCTTCGGCAGATCATCGAACAGTTAAG CAGTGGCTTCTTCTCCCCTAAACAGCCTGACCTGTTCAAGGACATCGTCAACATGCTCATGCACCATGAC CGGTTTAAAGTCTTTGCAGATTATGAGGAATACATTAAATGCCAGGACAAAGTCAGTGAGTTGTACAAGA ACCCAAGAGAGTGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAGTGACCGTAC CATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTTCTCGTCAGCGCCTGCCAGCCCCAGATGAG AAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209227 representing NM_012638
Red=Cloning site Green=Tags(s) MSRPLSDQDKRKQISVRGLAGVENVSDLKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIR TQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHT SQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPEL MRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFP GDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPR RWLVLCNPGLAEVIAERIGEEYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINP NSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDV VNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM AEEAGEDNFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHD RFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDE KI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012638 |
ORF Size | 2526 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012638.1, NP_036770.1 |
RefSeq Size | 2862 bp |
RefSeq ORF | 2529 bp |
Locus ID | 24701 |
MW | 97.3 kDa |
Gene Summary | catalyzes the hydrolysis of intracellular glycogen [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209227 | Pygm (untagged ORF) - Rat phosphorylase, glycogen, muscle (Pygm), (10 ug) |
CNY 10,740.00 |
|
RR209227L3 | Lenti ORF clone of Pygm (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, muscle (Pygm), (10 ug) |
CNY 12,640.00 |
|
RR209227L4 | Lenti ORF clone of Pygm (mGFP-tagged ORF) - Rat phosphorylase, glycogen, muscle (Pygm), (10 ug) |
CNY 13,780.00 |