Lars (NM_001009637) Rat Tagged ORF Clone
CAT#: RR209318
- TrueORF®
Lars (Myc-DDK-tagged ORF) - Rat leucyl-tRNA synthetase (Lars), (10 ug)
"NM_001009637" in other vectors (1)
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CNY 18,910.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC105585 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209318 representing NM_001009637
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAAGAAAAGGAACAGCCAAAGTAGACTTCTTGAAGAAGATTGAGAAAGAAGTCCAGCAGAAAT GGGAAGCGGAGAAAGTGTTTGAAGTCAGCGCGTCTTCACTGGAGAAGCAGAAGCAGAGCAGCAAGGGCAA ATACTTTGTCACCTTTCCATATCCATACATGAATGGACGCCTTCATTTGGGACACACGTTTTCCTTATCC AAGTGTGAGTTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTCCCCTTCGGGTTACACT GTACCGGTATGCCTATTAAGGCATGTGCTGACAAGCTGAAAAGGGAGATAGAGCTGTATGGCTGCCCTCC TGATTTTCCAGAGGAGGAGGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAATGAAGGAT AAAGCCAAAGGGAAAAAGAGTAAAGCCGCTGCTAAAGCAGGATCTTCTAAGTACCAGTGGAACATTATGA AATCCCTTGGGCTCTCTGATGAAGACATCGCGAAGTTTTCTGAAGCAGAACATTGGCTTGATTATTTCCC ACCACTGGCTGTTCAGGATCTGAAGAAAATAGGTTTGAAGGTAGACTGGCGGCGTTCCTTCATCACCACG GATGTGAATCCTTACTATGATTCTTTTGTCAGGTGGCAATTTTTAACTTTAAGAGAAAGAAATAAAATTA AATTCGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAAC CGGAGAGGGTGTTGGACCTCAGGAATATACCCTAGTCAAACTGAAGGTGCTTGATCCATACCCATCAAAA TTAAGTGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCCACACTCAGACCTGAGACTATGTTTGGAC AGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATATTCAT CTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAATGGCGTGGTGCCGGTT GTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACATGCTATAAGGTGA TCTATGTTCTCCCTATGCTGACCATTAAGGAGGATAAAGGCACTGGGGTGGTCACAAGCGTTCCTTCTGA TTCCCCGGATGATTTTGCGGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAGCAAAGTATGCAGTC AGAGATGACATGGTCCTGCCCTTTGAACCGGTGCCAGTCCTTGAAATCCCAGGGATTGGAAATCTTCCTG CTGTGACCGTTTGTGACGAACTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAGGCAAAGGA GAAATTGTATCTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGATTTAAAGGGCAGAAGATCCAA CATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAA AACAAGTGGTGTCGAGGTCTGCAGATGAGTGTGTTGTGGCCCTGTGTGACCAATGGTACTTGGATTATGG AGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGG AAGAATTTTGAAGCTTCCTTAGACTGGCTGCAAGAACATGCCTGCTCAAGAACTTACGGTTTAGGCACGC GTCTGCCTTGGGATGAGCAGTGGTTGATTGAATCCCTCTCAGACTCCACTATTTACATGGCGTTTTACAC AGTTGCACACCTGTTGCAGGGGGGTGACCTGCAGGGTCAGGCGGAGTCTCCACTGGGCATCAGACCCCAG CAGATGACGAGGGATGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCCAAGACTCAGATCCCAA AGGAAAAGCTGGATCAGCTGAAGCAGGAATTTGAGTTTTGGTATCCAGTGGACCTTCGAGCCTCTGGCAA GGATCTGATTCCAAATCATCTTTCATATTACATTTACAATCATGTGGCCATGTGGCCAGAACAAAGCGAC AAGTGGCCTGTATCCGTTCGAGCAAATGGACACCTGCTGCTCAACTCCGAGAAGATGTCAAAATCTACGG GAAACTTCCTCACTTTGAGCCAAGCTGTTGACAAGTTTTCCGCTGATGGAATGCGCCTGGCTCTGGCTGA TGCTGGCGACACTGTTGAAGATGCTAACTTTGTGGAAGCTATGGCAGACGCAGGCATTCTCCGTCTGTAC ACATGGGTAGAGTGGGTCAAAGAAATGCTTGCCAACTGCAGCAGCCTGAGAAGTGGACCTGCCAACTCAT TCAATGATCGTGTGTTTGCCAGTGAAATGAATGCAGGAATTATAAAAACAGATCAAAACTATGAAAAGAT GATGTTTAAAGAAGCGTTGAAAACAGGTTTTTTTGAGTTTCAGGCTGCGAAAGACAAGTACCGTGAACTG GCCACAGAGGGGATGCACAGAGAGCTTGTGTTCCGGTTTATTGAAGTTCAAACAATCCTTCTGACTCCAT TCTGTCCACATTTGTGTGAGCACATCTGGACACTGCTGGGAAAGCCTGATTCAATTATGCATGCCTCATG GCCTGTGGCAGGTCCCGTGGATGAATCCCTGATTCGTTCCTCACAGTACCTCATGGAAGTAGCACACGAC CTTAGATTACGGCTCAAGAATTACATGACGCCAGCTAAAGGGAAGAAGACGGACAAGCAACCAGTTCAGA GGCCCTCACACTGCACCATCTATGTGGCAAAGAACTACCCTGTCTGGCAGCACATTACCCTGACAACCCT ACGGAGCCACTTTGAGGCTAACAATGGAAAATTGCCTGATAACAAAGTCATCGCTTGTGAACTAGGCAGT TTGCCAGAACTGAAGAAATATATGAAGAAAGTTATGCCATTTGTTGCCATGATTAAGGAAAACATGGAGA AGAAAGGGCCGCGAGTCTTGGATTTAGAGTTGGAATTTGATGAGCAGGCAGTCCTTATGGAGAATATAGT CTACCTAACCAATTCTCTTGAGCTGGAACATATAGACGTCAAGTTTGCCTCTGAAGCTGAAGATAAAGTC AGGGAAGAGTGCTGTCCTGGGAAGCCACTTAATGTTTTCAGAACAGAACCTGGTGTGCCAGTTTCCCTAG TAAATCCTCAGCCATCCAGCGGGCACTTCTCTACCAAAATCGACATCAGACAAGGAGATAGCTGTGAGTC CATAATCAGACGCCTGACGAAAATGGACCGAGGAATCAAAGATCTCTCCAAAGTGAAACTCATGCGCTTT GATGATCCTCTGTTGGGGCCTCGACGAGTTCCAGTCCTGGGGAGAGAGCAGAACGAGAAGACACTCATTT CTGAGAATGCCATTTTCCACGTGGACCTAGTGAGCAAGAAAGTTCACCTTACTGAGAACGGGCTGCGCGC AGACATCGGTGACACAATAGTCTATCTGGTTCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR209318 representing NM_001009637
Red=Cloning site Green=Tags(s) MAERKGTAKVDFLKKIEKEVQQKWEAEKVFEVSASSLEKQKQSSKGKYFVTFPYPYMNGRLHLGHTFSLS KCEFAVGYQRLKGKSCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPEEEEEEEESSAKPGDIVMKD KAKGKKSKAAAKAGSSKYQWNIMKSLGLSDEDIAKFSEAEHWLDYFPPLAVQDLKKIGLKVDWRRSFITT DVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLDPYPSK LSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPV VKELMGEEILGASLSAPLTCYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDFAALRDLKKKQALRAKYAV RDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQ HVKKTIQKNMIDAGDALIYMEPEKQVVSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEESR KNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLQGQAESPLGIRPQ QMTRDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSD KWPVSVRANGHLLLNSEKMSKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLY TWVEWVKEMLANCSSLRSGPANSFNDRVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYREL ATEGMHRELVFRFIEVQTILLTPFCPHLCEHIWTLLGKPDSIMHASWPVAGPVDESLIRSSQYLMEVAHD LRLRLKNYMTPAKGKKTDKQPVQRPSHCTIYVAKNYPVWQHITLTTLRSHFEANNGKLPDNKVIACELGS LPELKKYMKKVMPFVAMIKENMEKKGPRVLDLELEFDEQAVLMENIVYLTNSLELEHIDVKFASEAEDKV REECCPGKPLNVFRTEPGVPVSLVNPQPSSGHFSTKIDIRQGDSCESIIRRLTKMDRGIKDLSKVKLMRF DDPLLGPRRVPVLGREQNEKTLISENAIFHVDLVSKKVHLTENGLRADIGDTIVYLVH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009637 |
ORF Size | 3534 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009637.1, NP_001009637.1 |
RefSeq Size | 3834 bp |
RefSeq ORF | 3537 bp |
Locus ID | 291624 |
MW | 134.3 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209318 | Lars (untagged ORF) - Rat leucyl-tRNA synthetase (Lars), (10 ug) |
CNY 18,910.00 |