Nsmaf (NM_181389) Rat Tagged ORF Clone
CAT#: RR209556
- TrueORF®
Nsmaf (Myc-DDK-tagged ORF) - Rat neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10 ug)
"NM_181389" in other vectors (3)
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CNY 11,880.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FAN |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209556 representing NM_181389
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCACCCGGAAGAGGCAGCGCGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGGTTCTCCTTAC TGCTGCTCAACTTGGAGGAGTACTACTTCGAGCAGCATACGGCTTTCCATGTCCAGCACCAGGGCAGCCC CGAGGAAAGGAAAATCAGAGGCTCCTTAAAGATATGTTCAAAATCAGTGATTTTTGAACCAGATGCAATA TCGCAGCCCATTCTTAAGATTCCTTTGAGAGACTGTTTAAAAATAGGAAAACATGGAGAAAATGGAGCCA ATAAACACTTTGCAAAGGCAAAATCTTTGGGTATTTCACTCATTTCCAGTCAGGTATATTTCATTAAGGA GCACAACATCGTGGCACCGTATAAGATAGAACGGGGAAAAATGGAGTATGTCTTCCAACTGGAAGTTTCT GGGAAGGTGGAAGATGTTGTGGAGACATTGCTACAGCTGCACAGAGCATCCTGCCTTGATAAACTTGGTG ACCAAATGGCCATGATAACAGCTATCTTGCAGTCACGTTTGGCTAGGACATCATTTGACAAAAACAGGTT CCAAAGCGTTTCAGAAAAGCTGCACATGGAGTGCAAAGCAGAGATGATGACGCCTCTGGTGACGAACTCT GGGCATGTGTGCATTACTGACACCAACCTGTATTTCCAGCCTCTCAACGGGTACCCGAAACCTGTGGTCC AGATAACGCTCCAAGATGTCCGGCGCATTTACAAAAGGAGGCACGGCCTCATGCCTTTGGGCTTGGAAGT GTTTTGCACAGAAGATGACCTGTGTTCTGACATATACCTAAAGTTCTATGAGCCTCAAGACAGAGATGAC CTCTATTTTTATATTGCCACGTACCTAGAACACCACGTGGCAGAACACACTGCGGAGAGCTACATGCTGC AGTGGCAGCGAGGGCACCTGTCCAACTACCAGTACCTGCTCCACTTCAACAACCTGGCCGACCGCGGCTG CAATGACCTGTCCCAGTACCCTGTGTTTCCATGGGTAATAAGTGACTACTCCAACCCAGAGCTAGATTTG TCAAATCCCGCAACCTTCCGAGATCTTAGTAAGCCAGTGGGAGCCCTAAATCCAGAGAGGCTGGAGAGAC TGCTGACTCGATACCAGGAAATGCCTGAGCCGAAGTTCATGTATGGGAGCCACTACTCTTCCCCAGGCTA TGTGCTCTTTTACCTCGTTAGGATCGCACCAGAATATATGCTGTGTCTACAAAATGGAAGATTTGATAAT GCAGATAGGATGTTCAACAGCATTGTAGAAACCTGGAAAAACTGTTTGGATGGTGCAACGAACTTCAAAG AGTTGATCCCAGAATTCTATGATGAAGACGCCAGCTTCTTGATCAATAGTCTGAAGTTGGATCTGGGGAA GAGACAAGGAGGGCAGATGGTTGACGACGTGGAGCTTCCTGCATGGGCCTCAAGTCCCCAAGACTTTCTC CAGAAGAACAAGGACGCCTTGGAAAGCAGTTATGTGTCTGAGCACCTTCATGAGTGGATTGATCTGATAT TTGGCTATAAGCAAAAAGGGAGTGAGGCCATAGGGGCCCATAACGTGTTTCACCCCCTGACCTATGAAGG CGGTGTAGACCTGAACAGCATTGAGGATCCCGATGACAAAGTAGCCATGCTGACCCAAATTTTGGAATTT GGGCAGACACCAAAACAGCTGTTTGTGACACCACATCCTCGAAGGATCACCCCCAAGTTTAAAAGTTTGT CCCAAGCGTCCAGCTACAATGCTTCTATGACAGACTCCCCAGTTTCTCCAGGGGAGGAGTCATTTGAAGA CCTGACTGAAGAGAGCAAAACTCTGGCCTGGAATAACATCACCAAGTTGCAGCTACATGAGCAGTACAAA ATCCACAAAGAGGCGGTTACAGGGATAGCAGTGTCTTGCAATGGGTCTTCAGTCTTTACCACCTCACAAG ATTCCACTTTGAAGATGTTTTCTAAGGAATCCAAAATGTTACAAAGAAGTATATCATTTTCAATATGGGC TTTATCGTCTTGTTTACTTTTGCCAGGAGACACCACTGTCATAAGTTCTTCTTGGGATAACAACGTCTAT TTTTATTCCATAGCATTTGGAAGACGCCAAGACACACTGATGGGACATGACGATGCTGTCAGTAAGATCT GTTGGCATAATGACAGGCTGTACTCTGCATCTTGGGACTCTACTGTGAAGGTGTGGTCCGGCGTTCCTGC AGAAATGCCAGGCACAAAAAGACACCAGTTTGACTTACTGGCTGAGCTTGAGCATGATGTCAGTGTCAAT ACGATCAATTTAAATGCTGTGAGTACACTGCTAGTTTCTGGCACCAAGGAAGGCCTGGTGAATATTTGGG ACCTCACTACAGCCACTCTATTACACCAGATTTCATGCCACTCAGGGACAGTATGTGATGCTGCTTTTAG CCCAGACAGTCGCCACGTCCTCAGCACAGGAGTAGACGGCTGCCTGAACGTGATTGATGTGCAGACGGGC ATGCTCATTTCCTCCATGGCCTCAGAGGAGCCGCAGAGGTGCTTTGTCTGGGATGGAAACTCTGTCGTAT CTGGAAGTCGGACTGGTGAGCTGCTTGTCTGGGACCTCCTTGGAGCAAAAGTCAGTGAGCGGATACAGGG CCACACAGGTGCCGTGACGTGCATACGGATGAATGAACAGTGTAGCAGCATCATCACCGGAGGGGAAGAC AGACAAGTTATGTTCTGGAAACTGCAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209556 representing NM_181389
Red=Cloning site Green=Tags(s) MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSPEERKIRGSLKICSKSVIFEPDAI SQPILKIPLRDCLKIGKHGENGANKHFAKAKSLGISLISSQVYFIKEHNIVAPYKIERGKMEYVFQLEVS GKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMMTPLVTNS GHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDD LYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHFNNLADRGCNDLSQYPVFPWVISDYSNPELDL SNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDN ADRMFNSIVETWKNCLDGATNFKELIPEFYDEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFL QKNKDALESSYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEF GQTPKQLFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQLHEQYK IHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSIWALSSCLLLPGDTTVISSSWDNNVY FYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVN TINLNAVSTLLVSGTKEGLVNIWDLTTATLLHQISCHSGTVCDAAFSPDSRHVLSTGVDGCLNVIDVQTG MLISSMASEEPQRCFVWDGNSVVSGSRTGELLVWDLLGAKVSERIQGHTGAVTCIRMNEQCSSIITGGED RQVMFWKLQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181389 |
ORF Size | 2760 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181389.2, NP_852054.1 |
RefSeq Size | 2787 bp |
RefSeq ORF | 2763 bp |
Locus ID | 353233 |
MW | 104.5 kDa |
Gene Summary | cytopasmic protein; mediates activation of sphingomyelinase and apoptosis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209556 | Nsmaf (untagged ORF) - Rat neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10 ug) |
CNY 11,880.00 |
|
RR209556L3 | Lenti ORF clone of Nsmaf (Myc-DDK-tagged ORF) - Rat neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10 ug) |
CNY 13,590.00 |
|
RR209556L4 | Lenti ORF clone of Nsmaf (mGFP-tagged ORF) - Rat neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), (10 ug) |
CNY 14,920.00 |