Prkaa1 (NM_019142) Rat Tagged ORF Clone
CAT#: RR210018
- TrueORF®
Prkaa1 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1), (10 ug)
"NM_019142" in other vectors (3)
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CNY 7,030.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AMPKalpha1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210018 representing NM_019142
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCAGACTCAGTTCCTGGAGAAAGATGGCGACGGCCGAGAAGCAGAAGCACGACGGGCGGGTGAAGA TCGGCCACTACATCCTGGGGGACACGCTGGGCGTCGGCACCTTCGGGAAAGTGAAGGTGGGCAAGCACGA GTTGACTGGACATAAAGTTGCTGTGAAGATACTCAACCGGCAGAAGATTCGAAGCCTGGACGTGGTCGGG AAAATCCGCAGAGAGATCCAGAACCTGAAGCTTTTCAGGCACCCTCATATAATCAAACTGTACCAGGTCA TCAGTACACCGTCTGATATTTTCATGGTCATGGAATATGTCTCAGGAGGAGAGCTATTTGATTATATCTG TAAAAATGGAAGGTTGGACGAAAAGGAGAGTCGACGTCTGTTCCAGCAGATCCTTTCTGGTGTGGACTAT TGTCACAGGCATATGGTGGTCCACAGAGATTTGAAACCTGAAAACGTCCTGCTTGATGCACACATGAATG CAAAGATAGCCGACTTCGGTCTTTCAAACATGATGTCAGATGGTGAATTTTTAAGAACGAGCTGTGGCTC GCCCAATTATGCTGCACCAGAAGTAATTTCAGGAAGATTGTACGCAGGCCCTGAAGTAGACATCTGGAGC AGCGGGGTCATTCTCTATGCTTTGCTGTGTGGAACTCTCCCTTTTGATGATGACCACGTGCCAACTCTTT TTAAGAAGATATGTGACGGGATATTTTATACCCCTCAGTATTTGAATCCCTCTGTAATAAGCCTTTTGAA GCATATGCTGCAGGTAGATCCTATGAAGAGGGCCACAATAAAAGATATCAGGGAACATGAATGGTTTAAG CAGGACCTTCCAAAATATCTCTTTCCTGAAGACCCGTCTTATAGTTCAACCATGATTGATGATGAAGCCT TAAAAGAAGTGTGTGAGAAGTTCGAGTGCTCAGAGGAGGAGGTCCTCAGCTGCCTGTACAACAGAAACCA CCAGGACCCACTGGCAGTTGCCTACCACCTCATAATAGACAACAGGAGAATAATGAACGAAGCCAAAGAT TTCTACTTGGCAACAAGCCCACCCGATTCTTTCCTCGATGATCACCATTTAACTCGGCCTCACCCTGAGA GAGTACCATTCTTGGTTGCCGAAACACCAAGGGCCCGACACACCCTAGATGAATTAAACCCACAGAAATC CAAACACCAAGGCGTACGGAAGGCAAAGTGGCATTTGGGGATTCGAAGTCAAAGCCGACCCAATGACATC ATGGCAGAAGTGTGTAGAGCAATCAAGCAGTTGGACTATGAATGGAAGGTTGTAAACCCCTATTATTTGC GTGTGCGAAGGAAGAACCCTGTGACAAGCACATTTTCCAAAATGAGTCTACAGCTATACCAAGTGGATAG TAGGACTTACTTATTGGATTTCCGAAGTATTGATGATGAGATTACAGAAGCCAAATCAGGGACTGCTACT CCACAGAGATCGGGATCCATCAGCAACTATCGATCTTGCCAAAGGAGCGACTCCGACGCCGAGGCTCAAG GAAAGCCCTCAGAAGTCTCTCTTACCTCATCCGTGACCTCCCTCGACTCCTCTCCTGTTGACGTAGCTCC AAGACCAGGAAGTCACACGATAGAATTTTTTGAAATGTGTGCAAATCTAATTAAAATTCTTGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210018 representing NM_019142
Red=Cloning site Green=Tags(s) MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVG KIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDY CHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWS SGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFK QDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKD FYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDI MAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTFSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTAT PQRSGSISNYRSCQRSDSDAEAQGKPSEVSLTSSVTSLDSSPVDVAPRPGSHTIEFFEMCANLIKILAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019142 |
ORF Size | 1677 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019142.2, NP_062015.2 |
RefSeq Size | 4674 bp |
RefSeq ORF | 1680 bp |
Locus ID | 65248 |
UniProt ID | P54645 |
MW | 64 kDa |
Gene Summary | catalytic subunit of AMP-activated protein kinase; phosphorylates hepatic acetyl-CoA carboxylase and adipose hormone-sensitive lipase [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210018 | Prkaa1 (untagged ORF) - Rat protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1), (10 ug) |
CNY 7,030.00 |
|
RR210018L3 | Lenti ORF clone of Prkaa1 (Myc-DDK-tagged ORF) - Rat protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1), (10 ug) |
CNY 8,930.00 |
|
RR210018L4 | Lenti ORF clone of Prkaa1 (mGFP-tagged ORF) - Rat protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1), (10 ug) |
CNY 9,790.00 |