Smarca4 (NM_134368) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	brg-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210055 representing NM_134368 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACTCCAGACCCACCCCTGGGTGGAACCCCTCGGCCTGGTCCTTCCCCAGGCCCTGGTCCTTCAC CTGGTGCAATGCTGGGTCCTAGCCCTGGCCCCTCACCAGGTTCTGCCCACAGCATGATGGGGCCAAGCCC AGGACCTCCTTCAGCAGGACATCCCATGCCCACCCAGGGGCCTGGAGGGTACCCCCAGGACAACATGCAT CAAATGCACAAGCCTATGGAGTCCATGCACGAGAAGGGCATGCCTGATGACCCACGATACAACCAAATGA AAGGGATGGGCATGCGATCAGGGGCCCACACAGGTATGGGACCTCCGCCTAGTCCCATGGACCAGCATTC TCAAGGTTACCCCTCACCCTTAGGTGGCTCTGAACATGCCTCCAGTCCTGTCCCGGCCAGCGGCCCATCT TCAGGCCCTCAGATGTCCTCTGGGCCAGGAGGGGCCCCACTAGATGGTTCTGACCCCCAGGCCTTGGGGC AGCAAAACAGAGGCCCAACCCCATTTAACCAGAACCAGCTGCATCAACTCAGAGCTCAGATAATGGCCTA TAAGATGTTGGCCAGGGGCCAGCCATTGCCCGACCACCTGCAGATGGCCGTTCAAGGCAAGCGGCCGATG CCTGGAATGCAGCAGCAGATGCCAACACTACCTCCACCTTCAGTGTCTGCCACAGGACCTGGACCCGGCC CTGGCCCTGGCCCTGGCCCAGGTCCAGGACCAGCCCCTCCGAATTACAGTAGACCCCATGGTATGGGAGG GCCCAACATGCCTCCTCCAGGACCCTCAGGTGTGCCCCCCGGGATGCCTGGTCAGCCTCCTGGAGGGCCT CCCAAGCCATGGCCTGAAGGACCCATGGCCAATGCTGCTGCCCCCACAAGCACCCCACAGAAGCTGATTC CCCCGCAACCAACAGGCCGCCCTTCACCTGCACCTCCTGCTGTCCCGCCTGCTGCCTCACCTGTAATGCC ACCACAAACACAGTCCCCAGGGCAGCCAGCCCAGCCTGCTCCATTGGTGCCATTGCACCAGAAGCAGAGC CGCATCACCCCCATCCAGAAGCCCCGGGGCCTGGACCCTGTGGAGATCCTACAGGAGCGGGAGTACAGGC TGCAGGCTCGAATCGCACACAGAATTCAGGAACTTGAAAACCTCCCTGGGTCCCTGGCTGGGGACCTTCG AACCAAAGCAACCATCGAACTCAAGGCCCTTAGGTTGCTGAACTTCCAGAGGCAGCTGCGCCAGGAGGTG GTGGTGTGCATGCGAAGAGACACAGCCCTAGAGACAGCCCTCAATGCCAAGGCTTATAAGCGCAGCAAAC GGCAGTCACTGCGGGAGGCCCGCATCACTGAGAAGCTGGAGAAGCAGCAGAAGATTGAACAGGAACGCAA GCGTCGTCAGAAGCACCAGGAGTACCTCAACAGCATTCTGCAGCATGCCAAGGACTTCAGGGAGTATCAC AGATCAGTCACAGGCAAACTCCAGAAACTCACCAAGGCTGTGGCCACCTACCATGCCAACACTGAGCGGG AGCAAAAGAAAGAAAATGAGCGCATCGAGAAGGAACGGATGCGAAGGCTTATGGCTGAAGACGAGGAGGG CTACCGCAAGCTCATTGACCAGAAGAAGGACAAGCGCCTGGCCTACCTCCTGCAGCAGACAGATGAGTAT GTGGCCAACCTCACAGAGCTGGTGCGGCAGCACAAAGCTGCCCAGGTAGCCAAGGAGAAGAAGAAGAAAA AGAAAAAGAAGAAGGCAGAAAATGCTGAAGGGCAGACACCTGCCATTGGACCCGACGGTGAGCCTCTGGA TGAGACCAGCCAGATGAGTGACCTCCCTGTGAAGGTGATCCATGTGGAGAGTGGCAAGATCCTCACTGGC ACAGATGCCCCAAAAGCCGGGCAGCTGGAAGCCTGGCTTGAGATGAACCCAGGGTATGAAGTAGCTCCCA GATCAGATAGTGAAGAAAGTGGCTCTGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGCAGCCTCAGCCCGC ACAGCCCCCTACACTGCCTGTGGAAGAAAAGAAGAAGATTCCAGACCCAGACAGCGATGATGTCTCTGAG GTGGACGCTCGACACATTATTGAGAACGCCAAGCAGGATGTGGACGATGAGTACGGCGTGTCCCAGGCCC TTGCTCGTGGTCTGCAGTCCTACTATGCTGTGGCCCATGCAGTGACAGAGAGAGTAGATAAGCAGTCAGC CCTCATGGTCAACGGTGTCCTCAAACAGTACCAGATCAAGGGTCTGGAGTGGCTGGTGTCCCTGTACAAC AACAACCTGAACGGCATCCTGGCTGATGAGATGGGGCTGGGGAAGACCATCCAAACCATTGCACTCATCA CGTACCTCATGGAGCACAAGCGCATCAATGGGCCCTTCCTCATCATCGTTCCTCTCTCGACGCTGTCAAA CTGGGCATATGAATTTGACAAGTGGGCCCCCTCTGTGGTGAAGGTTTCCTACAAGGGCTCTCCAGCTGCC AGACGAGCTTTTGTCCCCCAGCTTCGCAGCGGCAAGTTCAATGTCTTATTGACCACCTACGAGTATATCA TCAAAGACAAACATATCCTGGCCAAGATCCGCTGGAAGTACATGATTGTGGATGAAGGCCACCGCATGAA GAACCACCACTGCAAATTGACACAGGTCCTTAATACACACTACGTGGCCCCTCGGCGCCTGCTTCTCACA GGCACGCCACTGCAGAACAAGCTACCTGAGCTCTGGGCACTGCTGAACTTCCTGCTCCCCACTATCTTCA AGAGCTGCAGCACCTTCGAACAGTGGTTCAATGCACCCTTTGCCATGACTGGAGAGAAGGTGGACCTGAA TGAGGAGGAGACCATCCTCATTATTCGTCGTCTACACAAGGTGCTACGGCCCTTCCTGCTGCGGCGTCTC AAGAAGGAAGTTGAAGCCCAGCTCCCTGAGAAGGTAGAATATGTCATCAAATGTGACATGTCAGCCCTGC AGCGTGTGCTCTACCGTCACATGCAGGCCAAAGGTGTGCTGTTGACTGACGGCTCTGAGAAGGACAAGAA GGGCAAAGGTGGCACCAAGACGCTGATGAACACTATCATGCAGCTGCGCAAGATCTGCAACCACCCCTAC ATGTTCCAGCACATTGAGGAGTCCTTCTCTGAGCACCTGGGGTTCACCGGTGGCATCGTGCAAGGATTGG ACCTATATCGTGCCTCAGGGAAATTTGAACTTCTTGATAGAATTCTACCCAAACTCCGTGCAACAAACCA TAAAGTGCTACTCTTCTGCCAAATGACCTCCCTCATGACCATCATGGAAGACTACTTTGCATACCGTGGC TTCAAATACCTCAGGCTTGACGGAACCACAAAAGCAGAAGACCGGGGCATGCTGTTGAAAACCTTTAATG AGCCTGGCTCTGAGTATTTCATTTTCCTACTCAGTACCCGTGCTGGGGGGCTGGGCCTGAATCTGCAGTC AGCTGACACTGTGATCATCTTTGACAGTGACTGGAATCCTCACCAGGACCTGCAAGCACAGGATCGTGCC CATCGGATTGGGCAGCAGAATGAGGTGCGTGTGCTTCGCCTGTGCACCGTCAACAGTGTGGAAGAGAAGA TACTGGCTGCTGCTAAATACAAACTCAATGTGGATCAGAAGGTCATCCAGGCAGGCATGTTTGACCAGAA GTCTTCCAGCCATGAGAGGCGTGCCTTCCTGCAGGCCATCCTGGAGCACGAGGAGCAGGACGAGGAGGAA GATGAGGTGCCTGATGATGAGACCGTCAACCAGATGATTGCCCGGCACGAAGAAGAATTTGACCTCTTCA TGCGCATGGACTTGGACCGCCGGCGTGAAGAAGCCCGCAACCCCAAGCGGAAGCCACGCCTGATGGAAGA GGATGAGCTTCCATCCTGGATCATCAAGGATGATGCAGAGGTAGAGCGGCTGACATGTGAAGAGGAAGAG GAAAAGATGTTCGGCCGTGGTTCTCGCCACCGCAAGGAGGTAGACTACAGCGACTCACTGACAGAGAAGC AGTGGCTCAAGGCTATCGAGGAGGGCACGCTGGAGGAGATCGAAGAGGAGGTCCGGCAGAAGAAATCTTC ACGTAAGCGTAAGCGAGACAGCGAGGCCGGCTCCTCCACCCCGACCACCAGCACCCGCAGCCGTGACAAG GACGAGGAGAGCAAAAAGCAGAAGAAACGTGGGCGGCCACCTGCCGAGAAGCTGTCCCCAAACCCACCTA ACCTCACCAAGAAGATGAAGAAGATCGTGGACGCTGTGATCAAGTACAAAGACAGTAGTGGACGTCAGCT CAGTGAGGTGTTCATTCAGCTCCCCTCTCGCAAGGAACTTCCTGAGTACTATGAGCTCATCCGGAAACCT GTGGACTTCAAGAAGATCAAGGAACGCATCCGAAACCACAAGTACCGCAGCCTCAATGACCTGGAGAAGG ACGTGATGTTGCTGTGCCAGAATGCTCAGACCTTCAACCTCGAGGGCTCCCTGATCTACGAGGACTCCAT CGTCCTGCAGTCTGTCTTCACCAGCGTGCGGCAGAAAATCGAGAAGGAGGATGACAGTGAAGGCGAGGAA AGCGAGGAGGAAGAAGAGGGCGAGGAGGAAGGCTCTGAGTCTGAGTCCCGCTCTGTCAAGGTAAAGATCA AGCTGGGTCGAAAGGAGAAGGCCCAGGACCGACTCAAGGGGGGCAGGCGGCGGCCAAGCCGGGGGTCCCG GGCCAAGCCTGTCGTGAGTGACGATGACAGTGATGAGGAGCAGGAGGAGGACCGCTCAGGAAGTGGCAGC GAGGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210055 representing NM_134368 Red=Cloning site Green=Tags(s) MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPMPTQGPGGYPQDNMH QMHKPMESMHEKGMPDDPRYNQMKGMGMRSGAHTGMGPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPS SGPQMSSGPGGAPLDGSDPQALGQQNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPM PGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGP PKPWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPLVPLHQKQS RITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEV VVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFREYH RSVTGKLQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEY VANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTG TDAPKAGQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQPAQPPTLPVEEKKKIPDPDSDDVSE VDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYN NNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAA RRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLT GTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRL KKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPY MFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRG FKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRA HRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEE DEVPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEE EKMFGRGSRHRKEVDYSDSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSEAGSSTPTTSTRSRDK DEESKKQKKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSGRQLSEVFIQLPSRKELPEYYELIRKP VDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEDDSEGEE SEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSDEEQEEDRSGSGS EED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_134368
ORF Size	4839 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_134368.1, NP_599195.1
RefSeq Size	5568 bp
RefSeq ORF	4842 bp
Locus ID	171379
MW	181.4 kDa
Gene Summary	mouse homolog is a catalytic subunit of mammalian chromatin-remodeling complexes [RGD, Feb 2006]

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