Eif4g3 (NM_001106693) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210289 representing NM_001106693 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCACAACCTCAGGCCCGGTCTCCGCCCAGCAGAGCAGTGCCAGTACACTGCCCAGACAACTGGA AGAGGAGGAAGGTTTTGGAACAGACTCCCGTTTACAGGTCCCTGGCTGGAAGAGGCTGGATAAAATACTG CATTTTTTTCCAGAGGCCTCAAATCCAGCCTCCCAGAGCGGCCATCCCAAACAGCAGTCCTTCCCTCCGT CCTGGTGCGCAGACCCCCACCGCCGTGTATCAGGCCAATCAGCACATCATGATGGTTAACCATCTGCCTA TGCCATACCCAGTGCCCCAGGGGCCTCAGTACTGCATACCACAGTACCGCCACAGTGGCCCTCCGTATGT GGGGCCCCCACAGCAGTACCCAGTTCAGCCACCCGGCCCCGGGCCTTTTTATCCTGGACCAGGACCTGGG GACTTTGCTAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCAGTGTATCAGTCAGCACCTATCATAG TGCCTACGCAGCAGCAGCCCCCTCCAGCCAAGAGAGAGAAAAAAACTATACGAATTCGAGATCCAAATCA GGGAGGTAAAGACATAACCGAGGAGATTATGTCTGGAGGTGGCAGCAGAAATCCCACTCCACCTATAGGA AGACCCACATCCACACCCACTCCTCCTCAGCAGCTGCCCAGCCAGGCCCCTGAGCACAGCCCTGTGGTAT ATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGA AGAAAAGCCAAAACCAGACCCAGTGTTCCAGTCTCCTTCAACAGTCCTTAGGCTCGTGCTTAGTGGGGAG AAGAAAGAGCAAGCAGGCCAGATACCCGAGACTGCTGCAGGAGAGCCCAGCCCAGAGCCTCCTCGGACCT CATCACCCACCACCCTTCCTCCACTTGCCCGGAGTTCACTCCCTTCCCCCATGTCCGCTGCCCTTAGCAG CCAGCAGCTGCTCACTGCTGAGGACAAGTGTGAACTCTCTTCCTCAAAAGATGATGCCCCCCCTGTGCGC AGCCCCACGTCTTGCACAGCAGCACCAGGCCCTTCACTAACAGATGACATTGGTCTATGCAAGAAACCCT GTGGTGTAGCACCTCCTGACGGCCAGCTCGTTTCTAGTCCTGTCCTAATTAATGAAATGAACGGAGTTGG TGAGAAATTACCAGCCAAGGAGAACACTGTGGACATGGTGAGACAGGAAGTGTTGCCACTGACTCTTGAA CTGGAGATTCTGGAGCATCCCCAGGAAGAAAGGAAAGTGGAGTGTACCCCGACTCCCATCACCCCGTCCA CGATGCCTTCCTTCTCCCCAGCCCCTCCAACTCCGCCAGCTTCTCCTCCTTGTACTCCTGTTGTCCTGTC TGCCACCATTGCTCGATCTCCTGCTGTTGCCACTGTGATCCAGAGAGTTGTCGACGAGGGAGAGAGCCTG AGAACTTGTCTTAGCAAGGATGCCAAGGAGACACAGGACAAAGCGGAGGCAGAGTCAGATGGGCAAACAG AGGAGACTGCGGATCCCCAGAACGCACATGCAGGAAGGAGCCCTGCCCCAGTGCAAACAGCTACCACTGC GCCAAAGACGTGGAAGAAAACAAAAGAGCGGACCCGAACCCCAGATGAAGTGTTAGAAGCAGAGGCAGAG CCTAAAGCAGAAGAAGAGCTTTCAGTTGACAGTGTCCTTGAACCTGAGCAAGAGAAGATGAGCCAAGGGT TTCAGTTTGAGAGAGACCCCTCTACCCTGAAAAGAGCGAAAGCTGAGGAAGAAAACGGAGAAGAAGCAGA GCCTGTGCGCAATGGTGCTGAGAGTGCTTCCGAGGGGGAAGGAGGAGACGCTAATTCAGGCTCTACCGAT AGCTCTGGAGATGGGGTCACATTCCCATTTAAAGCAGAGTCCTGGAAACCTGCAGATACAGAAGGCAAGA AGCAATATGACCGGGAGTTCCTATTGGACATCCAGTTTATGCCTGCCTGTATCCAAAAACCAGAAGGCCT ACCTCCCATCAGCGATGTGGTTCTTGACAAGATCAACCAGCCCAGATTGCCAATGCGGACCCTGGATCCT CGAATTTTGCCTCGAGGACCTGATTTCACCCCAGCCTTTGCAGATTTTGGAAGACAGACGCCTGGTGGAA GAGGCGTGCCTTTGTTGAGTGTTGGACCTCGGAGGTCTCAACCTGGCCAGAGAAGGGAGCCCAGGAAGAT AATTACTGTCTCCGTAAAAGAAGACGTGCACTTGAGAAAGGCAGAGAATGCCTGGAAGCCCAGCCAGAAG CGGGACAGCCATGCCGATGACCCCGAAAGCATTAAAACGCAGGAACTTTTCAGAAAAGTTCGAAGTATCC TAAATAAACTGACACCACAGATGTTCAACCAGCTGATGAAGCAAGTGTCGGCACTTACCGTGGACACAGA GGAGCGGCTAAAGGGGGTCATCGACCTGGTCTTTGAGAAGGCTATCGATGAGCCCAGCTTCTCTGTGGCG TACGCAAACATGTGTCGGTGCCTAGTAACGCTGAAGGTACCTATGGCAGACAAGCCCGGTAACACAGTAA ATTTCCGGAAGCTGCTGCTTAATCGTTGCCAGAAGGAGTTTGAAAAGGATAAAGCAGATGATGACGTCTT TGAGAAGAAGCAGAAAGAACTGGAAGCTGCCAGTGCTCCAGAGGAAAGAACGAGGCTTCACGATGAACTG GAAGAAGCCAAGGACAAAGCCCGGCGGAGATCCATCGGCAACATCAAGTTCATTGGAGAGCTCTTTAAAC TCAAAATGCTGACGGAGGCCATCATGCATGATTGTGTGGTGAAGCTGCTAAAGAACCATGATGAGGAATC CCTGGAGTGCCTGTGTCGCCTGCTCACCACCATCGGCAAAGACCTGGACTTTGAGAAAGCAAAGCCGCGC ATGGACCAGTACTTTAACCAGATGGAGAAAATTGTCAAAGAAAGGAAAACTTCCTCCCGGATTCGGTTCA TGCTTCAGGATGTAATTGACCTCAGGCTGTGTAATTGGGTGTCCCGAAGAGCAGATCAGGGGCCAAAGAC TATCGAGCAGATTCACAAAGAGGCCAAAATAGAAGAACAAGAGGAGCAAAGGAAAGTCCAGCAGCTGATG ACCAAGGAGAAGAGAAGACCAGGCGTCCAAAGAGTGGATGAAGGTGGATGGAATACTGTACAAGGGGCCA AGAACAGTCGTGTACTGGACCCCTCCAAATTTTTGAAAATCACTAAGCCCACCATTGACGAGAAGATTCA GCTGGTTCCTAAGGCACAGCTGGGCAGCTGGGGCAAAGGCAGCAGTGGTGGAGCAAAGGCAAGCGAGACT GACGCCTTACGGTCAAGTGCTTCCAGTTTAAATAGATTCTCTCCTCTGCAACCTCCAGCGCCTTCAGGGT CCCCGTCAGCCACGCCTTTAGACTTCGATTCCCGGAGGGCGTTAACCAGTCGTGGGAGCATGGGCAGGGA GAAGAGCGACAAGCCGCTTCCAGCTGGAACAGCCCGTCCCAACACGTTCCTGAGGGGCAGCAGCAAAGAC CTGCTGGACAACCAGTCTCAGGAAGAGCAGCGCAGAGAGATGCTGGAGACGGTGAAGCAGCTGACAGGAG GCGTGGACGCCGAGCGGGCCAGCACTGAGGCCGACCGAAGCAAAGCGAGGGAGCTAGTCAAGTCAGAAGT GTGCACAGTGCCAGCCCCCGACAAGCCTGCACTGTCAGAAGAGGAAGTAGAGAGGAAGTCCAAGTCTATC ATTGACGAGTTCCTGCACATCAATGACTTTAAGGAGGCCACACAGTGCATAGAGGAGCTGAGTGCCCAGG GCCCACTGCATGTGTTCGTGAAGGTGGGTGTGGAGTTCACCCTGGAGCGGAGCCAGATCACCAGAGATCA CATGGGCCACTTACTGTATCAGCTGGTGCAGTCGGAAAAGCTCAGCAAGCAGGACTTTTTCAAAGGCTTT TCTGAAACCTTGGAGTTGGCAGATGACATGGCCATTGACATCCCCCATATTTGGTTGTACCTGGCTGAAC TAGTCACCCCCATGTTAAAAGAAGGGGGAATCTCCATGAGAGAACTTATTGTGGAATTCAGCAAGCCATT ACTTCCTGTTGGTAGAGCTGGGGTCCTGCTTTCCGAAATCCTGCACCTTCTATGCAGACAAATGAAGTTG GACTTCACGGAGTCAGAGGGACCCTGCTCCTCTGAAGCACTTTCAAAGAAAGAATTGTCTGCTGAGGAGC TGTCTCAGCGCCTGGAGAAACTGATCATGGAGGACAAAGCAGATGACGAGCGGATCTTTGACTGGGTGGA GGCCAATCTAGACGAAAGCCAGATGAGTTCGCCCACATTCCTTAGAGCTTTAATGACGGCCGTTTGCAAA GCAGCCATTGTCGCCGACTGTTCTACCTTCAGAGTGGACACTGCTGTGATCAAGCAGAGAGTGCCGATCT TACTCAAGTACCTGGACTCAGACACAGAGAAGGAGCTGCAAGCACTTTATGCACTACAAGCATCAATAGT GAAACTTGACCAACCTGCCAATTTGCTCCGGATGTTTTTTGATTGCCTGTATGATGAGGAGGTGATATCA GAGGATGCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCTGAACAAAACGGGAAGGGCGTAGCCCTCA AATCTGTCACAGCGTTTTTCACGTGGCTTCGGGAAGCAGAAGAGGAGTCGGAGGATAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210289 representing NM_001106693 Red=Cloning site Green=Tags(s) MNSQPQARSPPSRAVPVHCPDNWKRRKVLEQTPVYRSLAGRGWIKYCIFFQRPQIQPPRAAIPNSSPSLR PGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPG DFANAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIG RPTSTPTPPQQLPSQAPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVFQSPSTVLRLVLSGE KKEQAGQIPETAAGEPSPEPPRTSSPTTLPPLARSSLPSPMSAALSSQQLLTAEDKCELSSSKDDAPPVR SPTSCTAAPGPSLTDDIGLCKKPCGVAPPDGQLVSSPVLINEMNGVGEKLPAKENTVDMVRQEVLPLTLE LEILEHPQEERKVECTPTPITPSTMPSFSPAPPTPPASPPCTPVVLSATIARSPAVATVIQRVVDEGESL RTCLSKDAKETQDKAEAESDGQTEETADPQNAHAGRSPAPVQTATTAPKTWKKTKERTRTPDEVLEAEAE PKAEEELSVDSVLEPEQEKMSQGFQFERDPSTLKRAKAEEENGEEAEPVRNGAESASEGEGGDANSGSTD SSGDGVTFPFKAESWKPADTEGKKQYDREFLLDIQFMPACIQKPEGLPPISDVVLDKINQPRLPMRTLDP RILPRGPDFTPAFADFGRQTPGGRGVPLLSVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQK RDSHADDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVA YANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDEL EEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPR MDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLM TKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASET DALRSSASSLNRFSPLQPPAPSGSPSATPLDFDSRRALTSRGSMGREKSDKPLPAGTARPNTFLRGSSKD LLDNQSQEEQRREMLETVKQLTGGVDAERASTEADRSKARELVKSEVCTVPAPDKPALSEEEVERKSKSI IDEFLHINDFKEATQCIEELSAQGPLHVFVKVGVEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGF SETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMKL DFTESEGPCSSEALSKKELSAEELSQRLEKLIMEDKADDERIFDWVEANLDESQMSSPTFLRALMTAVCK AAIVADCSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVIS EDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106693
ORF Size	4749 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106693.1, NP_001100163.1
RefSeq Size	5307 bp
RefSeq ORF	4752 bp
Locus ID	298573
MW	175.4 kDa

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