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Igsf5 (NM_001006990) Rat Tagged ORF Clone
CAT＃: RR210338
- TrueORF®
Igsf5 (Myc-DDK-tagged ORF) - Rat cell adhesion molecule JCAM (LOC304000), (10 ug)
"NM_001006990" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Jam4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210338 representing NM_001006990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCAGCTGGAGAGATGTCCTGGCTGTGCTGGTCATCCTGGCTCAGTTGACAGTTTCCGGATCCA GTTATCAGATCATAGAAGGTCCTCGGAATGTAACAGCCCTAAAGGGCTCGGAGGCTCGCTTCAACTGCAC TGTGACTCATGGCTGGAAGCTTATCATGTGGGCTCTCAACGGTATCGTGGAGCTGAGTCTCACCTCCCAG AAACCCATCATCACCAGCAACCGCTTCACCTCTGCCAGCTACAACAGTACTGACAGCTTCATCTCTGAGA TGATCATCCACAATGTACAGCTCAACGACTCGGGGCCCGTGCAATGTAGCCTGCAGAACAGCAATGTGTT TGGATTCGCCTTTCTCTCGGTGCAAGTCATGGGGACTCTGAACATTCCTAGCAGCAACCTTATAGTCACT GAGGGTGAGCCCTGTAACGTGACTTGCTATGCCGTGGGCTGGACCCCACTCCCGAATATTTCCTGGGAGC TTGAGGTTCCTGTAAGTCATTCAAGCTACTATTCCTTTCTGGAGCCGGGCAACTCTCTGAGGGTCTTGAG TGTCCTGGATCTCACACCACTGGGCAACGGGACCTTGACTTGTGTGGCAGAGATGAAGGACTTGCAGGCC AGCGAGTCCTTAACTGTCAACCTGACTGTGGTTCAGCCTCCACCGGACAGTATTGGCGGGGAAGGCCAAG CGCTGCCAACCTGGGCCATCATCCTCCTGGCAGTGGCCTTTTCCTTACTCTTGATCCTGATCATTGCTCT GATTATAATATTCTGTTGCTGTTGTGTCTCCAGGAGAGAAAAGGAAGAATCTACTTATCAAAATGAAATA AGGAAATCTGCAAATGTGAGGACAGGCAAAGCAGATCCCGAGACATGGTTAAAAAGTGGGAAGGAAAACT ATGGGTACAAATCGGATGAAGCAAGGGCTGCACAAATTGCTTCTCTCCCTCCTAAATCTGGTGAAGTTAG CCTTCCAGAACAACGCAGTAGCCTTCCTCAACAGGAACTTGATAAACATCGGCCCAGCCCAGTGACTCAT CCACGAGTTTCCTTTGACATCGCCAGTCCTCAGAAGATCAGAAATGTGACTATAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210338 representing NM_001006990
Red=Cloning site Green=Tags(s) MEGSWRDVLAVLVILAQLTVSGSSYQIIEGPRNVTALKGSEARFNCTVTHGWKLIMWALNGIVELSLTSQ KPIITSNRFTSASYNSTDSFISEMIIHNVQLNDSGPVQCSLQNSNVFGFAFLSVQVMGTLNIPSSNLIVT EGEPCNVTCYAVGWTPLPNISWELEVPVSHSSYYSFLEPGNSLRVLSVLDLTPLGNGTLTCVAEMKDLQA SESLTVNLTVVQPPPDSIGGEGQALPTWAIILLAVAFSLLLILIIALIIIFCCCCVSRREKEESTYQNEI RKSANVRTGKADPETWLKSGKENYGYKSDEARAAQIASLPPKSGEVSLPEQRSSLPQQELDKHRPSPVTH PRVSFDIASPQKIRNVTIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001006990 |
| ORF Size | 1107 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001006990.2, NP_001006991.1 |
| RefSeq Size | 2164 bp |
| RefSeq ORF | 1110 bp |
| Locus ID | 304000 |
| UniProt ID | Q5VJ70 |
| MW | 40.3 kDa |
| Gene Summary | Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210338 | Igsf5 (untagged ORF) - Rat cell adhesion molecule JCAM (LOC304000), (10 ug) |
CNY 3,990.00 |
|
| RR210338L3 | Lenti ORF clone of Igsf5 (Myc-DDK-tagged ORF) - Rat cell adhesion molecule JCAM (LOC304000), (10 ug) |
CNY 6,080.00 |
|
| RR210338L4 | Lenti ORF clone of Igsf5 (mGFP-tagged ORF) - Rat cell adhesion molecule JCAM (LOC304000), (10 ug) |
CNY 6,650.00 |
