Madd (NM_053585) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210404 representing NM_053585 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAAAAGAAGTTCTGCCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCATCCAAGCA GTGACAGTGTGGCTCAGACTCCTGAACTTCTGCGGAGGTACCCACTAGAGGACCACCCGGAGTTTCCCCT GCCCCCAGATGTGGTGTTCTTCTGCCAGCCAGAGGGATGTCTGAGCGTGCGGCAACGGCGCATGAGCCTC CGAGATGATACCTCTTTTGTCTTCACCCTAACCGATAAGGACACTGGAGTCACCCGTTATGGCATCTGTG TCAACTTCTACCGTTCCTTTCAAAAGCGAATGCCAAAGGAAAAGGCGGAAGGCGGAGCAGGACCCCGTGG GAAGGAAGGAGCTCATGCCCCCTGTGCCTCAGAAGAGGCCGCCACCGAGAGCTCAGAGAGTGGCTCAACC TTGCAGCCTCCTAGTGCTGACTCCACTCCTGACGTAAACCAGTCTCCTCGGGGCAAACGTAGGGCAAAAG CAGGCAACCGCTCCCGAAACAGTACCCTGACATCCCTGTGTGTGCTTAGCCACTACCCTTTCTTCTCTAC CTTCAGAGAGTGTCTGTATACTCTCAAACGTCTGGTAGACTGCTGTAGTGAACGGCTCCTAGGCAAGAAA CCGGGCATCCCTCGAGGTGTACAAAGGGACACCATGTGGCGAATCTTTACTGGATCACTGCTAGTGGAGG AGAAGTCCAGTGCCCTTCTGCATGACCTTCGAGAGATCGAGGCCTGGATCTATCGGTTGCTACGCTCCCC GGTGCCTGTCTCTGGGCAGAAGCGAGTGGACATTGAGGTCCTACCCCAGGAAGTGCAGCAGGCCCTGACA TTTGCTCTTCCAGATCCATCCCGGTTCACCCTAGTAGATTTCCCTCTTCACCTTCCCTTGGAACTTCTGG GTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCTACAGTCTCGAGA CTACAACGCACTCTCCATGTCTGTGATGGCGTTTGTGGCAATGATCTACCCCCTGGAGTACATGTTCCCT GTCATCCCGCTGCTTCCCACCTGCATGGCTTCGGCAGAGCAGCTACTCTTGGCTCCAACCCCATATATCA TTGGAGTCCCTGCCAGCTTTTTCCTCTATAAGCTAGACTTCAAAATGCCTGATGACGTGTGGCTGGTGGA TCTGGACAGCAACAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCAGAACCGGAATCA TTAGAGCTGAAAAAACACTTAAAGCAGGCTCTAGCCAGCATGAGTCTCAACACCCAGCCCATTCTTAATC TGGAGAAATTCCATGAGGGCCAGGAGACACCACTTCTCTTGGGAAGGTTTTCTAATGACCTTCAGTCTAC ACCTTCCACTGAATTCAACCCACTCATTTATGGCAATGATGTAGATTCAGTCGATGTTGCAACGAGAGTG GCCATGGTTCGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACACGTACCCTGCGAC TCTTCCCCCGGCCCGTGGTTGCCTTCCAAGCTGGCTCCTTTCTGGCCTCACGCCCCCGGCAGACCCCCTT TGCTGAGAAACTGGCCAGGACTCAAGCCGTGGAGTACTTTGGAGAATGGATCCTGAACCCTTCTAACTAT GCCTTCCAGCGGATTCACAACAATACGTTTGATCCAGCCCTCATTGGTGACAAGCCTAAGTGGTACGCTC ACCAGCTGCAGCCCATTCACTATCGAGTCTATGACAGCAATTCTCAGCTGGCTGAGGCCCTGAGTGTGCC ACCGGAGCGTGATTCTGAGTCTGACCCCACTGATGACAGTGGCAGTGACAGCATGGACTATGATGACTCA AGCTCTTCTTACTCCTCCCTGGGGGACTTCGTCAGTGAAATGATGAAGTGTGACATCAATGGCGACACTC CCAATGTGGATCCTCTGACGCATGCGGCCCTGGGAGACGCCAGTGAAGTGGAGATTGATGAGCTGCAGCC CCAGAAGGAGGGAGAGGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAGAACCTTCCCCTGCGCTCCAGC TCCAGCACCACTGCCAGCAGTAGCCCTAGCACCATCGTCCACGGCGCCCACTCTGAACCTGCTGATTCTA CAGAGGTGGGAGATAAGGCAGCAACAGGCATCTCCAAGCCCCTCCCTCCTGTGCCTCCCAGCATTTGCAA ATCTACTGTGGACAGGAGACAGACAGAGACTGGAGAGGGGTCAGTGTGCCAGCGAACCTACGACCATCCA TATTTTGAGCCCCAATATGGCTCCCCCGCTGAGGAAGATGATGACGAGCAGGGAGAGAGCTACACGCCCC GGTTCAGCCAACATGCCAGTGGCAGTCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAAGCTGGCCAG TGACTCAGACGCAGAGTCAGACTCCCGAGCAAGCTCTCCCAACTCCACCGTCTCTAACAACAGCACCGAG GGCTTCGGGGGCATCATGTCTTTTGCTAGCAGCCTGTATCGGAACCATAGTACAAGCTTCAGCCTTTCAA ACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGACTACACCATTCCCCAGCCTGAAAGGAAACAGGAG GGCCTTAGTGGACCAGAAATCATCTGTCATTAAACACAGCCCAACTGTGAAAAGAGAACCTCCATCACCG CAGGGCCGGTCCAGCAATTCTAGCGAGAACCAACAGTTCCTGAAGGAGGTGGTCCACAGTGTGCTGGATG GCCAGGGAGTCGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGTGAGCAGCTCCGAGTCTT TGTACTGAGCAAGCTGAGTCGTGCAGTGCAGTCAGAGGATGATGCCCGACAGGATGTCATCCAGGATGTG GAGATCAGTCGGAAGGTGTACAAGGGAATGCTAGACCTCCTCAAGTGCACAGTCCTCAGCCTCGAGCAGT CCTATGCCCATGCTGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCATTA CTATAGTAAAGAACCAGACAAACGCAAGAGAAGTCCAACAGAGAATGTAAATACCCCAGTTGGCAAGGAT CCTGGTCTGGCTGGGCGGGGGGACCCAAAGGCAATGGCTCAGCTAAGGGTCCCTCAGCTGGGTCCTCGGG CACCAAGTGCTACAGGAAGGGGTCCTAAAGAACTGGACACCAGAAGTTTAAAGGAAGAGAATTTTGTAGC GTCTGTGGGACCAGAGGTCATCAAGCCTGTCTTTGACCTGGGTGAGACTGAGGAGAAAAAGTCCCAGATC AGCGCAGACAGTGGTGTGAGCCTGGCATCTGCTTCCCAGAGGACTGATCAAGACTCTGTCATCGGTGTGA GTCCAGCTGTTATGATCCGAAGCTCAAGTCAGGATTCTGAAGTGAGTAACAGTTCAGGAGAGACCCTCGG AGCAGACAGTGACCTGAGCAGCAATGCAGGTGATGGGCCAGGAGGCGAAGGCAGTGCCCACTTGGCAAGT TCTCGGGCTACTCTGTCTGACAGTGAAATTGAAACCAATTCTGCTACAAGCACCATCTTTGGTAAAGCCC ATAGCTTGAAGCCAAAGGAGAAGCCGGCTAGTAGTCCAGTTCGCTCTTCCGAAGATGTAAGCCAGCGAGT CTATCTCTATGAGGGACTACTAGGAAGGGACAAAGGATCCATGTGGGACCAGTTAGAGGATGCTGCTATG GAGACCTTTTCTATAAGCAAAGAGCGGTCTACTTTATGGGACCAAATGCAGTTCTGGGAAGATGCGTTCC TAGATGCTGTGATGTTGGAAAGAGAAGGAATGGGTATGGACCAGGGCCCTCAGGAAATGATCGACAGGTA CCTGTCCCTAGGAGAGCATGACCGGAAGCGCCTGGAGGATGATGAAGATCGCTTACTGGCCACACTCTTA CACAACCTCATATCCTATATGCTCCTGATGAAGGTGAACAAGAACGACATCAGGAAGAAAGTACGGCGCC TAATGGGAAAGTCCCACGTTGGGCTGGTGTACAGTCAACAAATCAATGAGGTGCTCGATCAGCTGACCAA CCTGAATGGGCGTGATCTCTCCATCCGATCCAGTGGCAGCAGGCACATGAAGAAGCAGACGTTCGTTGTG CACGCAGGGACGGACACAAATGGAGATATCTTTTTCATGGAAGTGTGTGACGACTGTGTGGTCTTACGTA GTAACATCGGGACAGTGTACGAGCGCTGGTGGTATGAGAAGCTTATCAACATGACCTACTGTCCCAAGAC CAAGGTCCTGTGCTTATGGCGTAGAAATGGCTCTGAGACGCAGCTCAACAAGTTCTATACCAAGAAGTGT CGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGTGCGGCTGCCCGGCAACAAAGCATCAAACCCG GGCCTGAACTAGGTGGTGAGTTCCCTGTGCAGGACATGAAGACTGGAGAGGGCGGCTTGCTTCAGGTCAC CCTGGAAGGGATCAATCTCAAGTTCATGCATAACCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGC AATACAGTTCGAGGCGTCTTTGTCCTGGAAGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGT ACAAGACTCCAATGGCCCACGAGATCTGCTATTCTGTGTTGTGTCTCTTCTCGTATGTGGCCGCAGTTCG TAGCAGTGAAGAAGACCTCCGAACCCCACCTCGGCCTGTCTCTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210404 representing NM_053585 Red=Cloning site Green=Tags(s) MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSL RDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKAEGGAGPRGKEGAHAPCASEEAATESSESGST LQPPSADSTPDVNQSPRGKRRAKAGNRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKK PGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQEVQQALT FALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFP VIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPES LELKKHLKQALASMSLNTQPILNLEKFHEGQETPLLLGRFSNDLQSTPSTEFNPLIYGNDVDSVDVATRV AMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNY AFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSESDPTDDSGSDSMDYDDS SSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENLPLRSS SSTTASSSPSTIVHGAHSEPADSTEVGDKAATGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDHP YFEPQYGSPAEEDDDEQGESYTPRFSQHASGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTE GFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSP QGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLSRAVQSEDDARQDVIQDV EISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTENVNTPVGKD PGLAGRGDPKAMAQLRVPQLGPRAPSATGRGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQI SADSGVSLASASQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLAS SRATLSDSEIETNSATSTIFGKAHSLKPKEKPASSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAM ETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLL HNLISYMLLMKVNKNDIRKKVRRLMGKSHVGLVYSQQINEVLDQLTNLNGRDLSIRSSGSRHMKKQTFVV HAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKC RELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKC NTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053585
ORF Size	4806 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053585.1, NP_446037.1
RefSeq Size	5249 bp
RefSeq ORF	4809 bp
Locus ID	94193
UniProt ID	O08873
MW	178 kDa
Gene Summary	GDP/GTP exchange protein (GEP) for Rab3 subfamily small G proteins; may play a role in intracellular vesicle trafficking [RGD, Feb 2006]

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