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Chrnb1 (NM_012528) Rat Tagged ORF Clone
CAT＃: RR210553
- TrueORF®
Chrnb1 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, beta 1 (muscle) (Chrnb1), (10 ug)
"NM_012528" in other vectors (3)
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CNY 6,460.00
Product images
CNY 300.00
CNY 11,000.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Acrb; nAChR; RNACRB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210553 representing NM_012528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTAGGGGCGCTGCTTCTGATTCTGGGGATACTCGGGACGCCCCTCGCCCCAGGAGCCCGCGGGT CGGAAGCCGAAGGCCAACTGCTTAAGAAACTTTTCTCGGATTATGATAGCTCAGTAAGGCCGGCGCAGGA GGTGGGAGACCGCGTCGGGGTCAGCATCGGCCTCACCCTGGCGCAACTCATCAGCCTGAACGAGAAGGAT GAAGAAATGAGCACAAAGGTGTACTTAGACCTGGAGTGGACCGACTACAGGTTAAGCTGGGACCCCGCAG AACACGATGGCATCGAGTCTCTCCGTGTCACTGCTGAATCTGTTTGGCTCCCGGACGTGGTGCTGCTGAA CAACAATGATGGAAACTTTGACGTTGCCCTGGACATTAATGTCGTGGTGTCCTTTGAGGGCTCTGTGCGC TGGCAACCCCCTGGCCTGTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTTGACTGGCAGA ATTGCACCATGGTGTTTAGTTCCTACAGCTATGACAGCTCTGAGGTCAGCCTGAAGACAGGCCCAGATCC TGATGGACAGGAGAGGCAGGAAATCTACATCCATGAAGGGACCTTCATCGAGAATGGTCAATGGGAGATT ATCCACAAACCCTCTAGGTTAATCCACCTTCCAGGGGATCGAAGAGGAGGGAAGGAAGGACATCGTGAGG AAGTTATCTTCTATCTCATTATTCGGAGGAAGCCTCTCTTCTACCTGGTCAATGTCATTGCCCCGTGCAT CCTCATCACCCTCCTGGCCATCTTTGTTTTCTACCTTCCACCAGATGCAGGAGAGAAGATGGGGCTCTCC ATCTTTGCCCTGCTGACCCTCACTGTGTTCTTGCTGCTGCTGGCCGACAAAGTGCCTGAGACCTCCCTGG CGGTCCCCATCATCATCAAGTATCTCATGTTTACCATGATCCTTGTCACCTTCTCAGTTATCCTTAGCGT TGTGGTCCTCAACTTGCACCATCGCTCACCCCACACCCACCAAATGCCCTTTTGGGTCCGCCAGATCTTC ATTCACAAGCTCCCTCCATACCTGGGTCTGAAGAGGCCCAAACCCGAGAGAGACCAACTACCCGAACCAC ATCACTCCTTCTCCCCAAGGAGTGGCTGGGGCCGAGGAACTGATGAATATTTCATACGGAAGCCTCCATG TGATTTTCTTTTCCCTAAACTTAACAGGTTTCAGCCTGAATCACCTGCTCCGGACCTGAGACGATTTATC GATGGTCCACCCCGGGCTGTAGGTCTGCCTCAGGAGCTACGCGAGGTCATTTCTTCCATCAGCTACATGG CCCGACAACTTCAGGAACAGGAGGACCACGATGCACTGAAGGAGGACTGGCAGTTTGTCGCCATGGTTGT GGACCGTCTTTTTCTGTGGACATTCATCGTTTTCACAAGCGTCGGGACGCTAGTCATTTTCCTAGATGCC ACGTACCATTTGCCCCCTCCTGAACCTTTTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210553 representing NM_012528
Red=Cloning site Green=Tags(s) MALGALLLILGILGTPLAPGARGSEAEGQLLKKLFSDYDSSVRPAQEVGDRVGVSIGLTLAQLISLNEKD EEMSTKVYLDLEWTDYRLSWDPAEHDGIESLRVTAESVWLPDVVLLNNNDGNFDVALDINVVVSFEGSVR WQPPGLYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLKTGPDPDGQERQEIYIHEGTFIENGQWEI IHKPSRLIHLPGDRRGGKEGHREEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLS IFALLTLTVFLLLLADKVPETSLAVPIIIKYLMFTMILVTFSVILSVVVLNLHHRSPHTHQMPFWVRQIF IHKLPPYLGLKRPKPERDQLPEPHHSFSPRSGWGRGTDEYFIRKPPCDFLFPKLNRFQPESPAPDLRRFI DGPPRAVGLPQELREVISSISYMARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIVFTSVGTLVIFLDA TYHLPPPEPFP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012528 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012528.1, NP_036660.1 |
| RefSeq Size | 2283 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 24261 |
| UniProt ID | P25109 |
| MW | 57 kDa |
| Gene Summary | beta subunit of the nicotinic acetylcholine receptor; can assemble into functionally different acetylcholine receptor channel subtypes [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210553 | Chrnb1 (untagged ORF) - Rat cholinergic receptor, nicotinic, beta 1 (muscle) (Chrnb1), (10 ug) |
CNY 6,460.00 |
|
| RR210553L3 | Lenti ORF clone of Chrnb1 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, beta 1 (muscle) (Chrnb1), (10 ug) |
CNY 8,360.00 |
|
| RR210553L4 | Lenti ORF clone of Chrnb1 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, beta 1 (muscle) (Chrnb1), (10 ug) |
CNY 9,120.00 |
