Lamc3 (NM_001107830) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210588 representing NM_001107830 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGTCCGGGATCCTGTCCCGCCTGGCAACGGTGGTATGTGTGGCGTTGGTGATGCTGAAGGCGC ACTGCGCGACGGGTGCGGGCATGGGCTCTTGCTACGACGGTGCGGGACGCGCACAGCGCTGTCTGCCTGA GTTCGAGAACGCGGCGTTCGGCCGACGCGCCGAGGCCTCGCACACGTGCGGACAGCCCCCAGAGGACTTC TGTCCGCACGTGGGGGCACCAGGAGCTGGGCCACAGTGCCAGCGCTGTGATGATGCTGACCCCCGGCGAC GCCACGACGCCTCCTACCTCACAGACTTCCACAGCCCTGATGACAGCACCTGGTGGCAAAGTCCGTCCAT GGCCTTCGGGGTGCAGTATCCCACTTCGGTTAACCTGACCCTGCATCTAGGGAAGGCCTATGAGATCACC TATGTGAGGCTAAAGTTCCACACCAGCCGCCCCGAGAGCTTTGCCATCTACAAGCGCACGCACACGGGTG GCCCCTGGGAGCCCTACCAATACTACAGTGCCTCCTGCCAGAAAACCTATGGCCATCCTGAGGGCCACTA CCTGCGACCTGGTGAAGATGAGAGGGTGGCTTTCTGCACCTCTGAGTTCAGCGACATCTCCCCCCTGAAT GGGGGCAACGTGGCCTTCTCCACTCTGGAGGGCCGTCCCAGTGCCTACAACTTTGAGGAGAGCCCTGTGC TGCAGGAGTGGGTTACCAGCACCGATCTCCTGATCTCTCTGGATCGTCTCAACACGTTTGGGGATGACAT CTTCAAGGACCCCAGAGTGCTCCAGTCTTACTACTACGCTGTGTCTGACTTCTCTGTGGGTGGCAGGTGC AAATGCAATGGTCACGCCAGTGCGTGCGGGCCCAACGAGGCTGGTCAGCTGGCTTGTCACTGCCAGCACA ACACCACGGGAGTGGACTGTGAGCGTTGTCTACCCTTCTTCCAGGACCGTCCGTGGGCCCGCGGCACCGC AGAGGATGCCAACGAGTGTCTGCCCTGCAACTGTAGTGGGCACTCTGAGGAGTGCACGTTTGACAGGGAG CTCTATCGGAGCACGGGCCATGGTGGGCACTGCCAGCGCTGCCGAGACCACACAGCTGGGCCACATTGTG AGCATTGTGAGAAGAACTATTACAGATGGGACCCGAAGACACCATGCCAACCCTGTGACTGCCACCCAGC AGGCTCCCTGAGTCTCCAGTGTGACAACTCGGGCACCTGTCCCTGCAAGCTGACGGTGACTGGCTGGAAG TGTGATCGCTGCCTGCCTGGATTCCACTCACTCAGTGAGGGCGGCTGCAGACCCTGTACCTGCAATGTCG CTGGCAGCTTGGGCACCTGCGATCCCCGAAGTGGAAACTGCCCCTGCAAAGAGAATGTGGAAGGCGGTCT GTGTGACAGATGCCGCCCTGGGACTTTTAACCTGCAGCCCCATAACCCAGCCGGCTGCAGTAGCTGCTTC TGTTACGGCCACTCCAAGGTGTGTGCCCCTGCTTCAGGGTTCCAGGAACACCACATCCGCTCAGACTTCC GCCATGGAGCTGATGGCTGGCAGGCCAGAAGCACAGAGGTGTCAGAGCGTCCTCTGCAATGGAGCCAGAG CGGGATCCTCCTGGGCCTGCGAGGAGGGGAGGAACTCTCAGCCCCAGAGAAGTTCCTGGGAGACCAGAGA CTCAGCTATGGACAGCCACTCATACTGACCCTCCAAGTACCCCCCGGGGGCTCCCCACCTCCTATGCAGC TGAGACTGGAGGGGGCAGGTTTATCTCTGGCTCTGAAACCCTCCAATCTACCCAGCTCTCAGGACACCAG GCAGCCAGGACGAGTTCAGCTCCAGTTCCTCCTGCAGGAGACTTCTGAAGAGGCAGAGCCTCCACTGCCC GCCTTCCACTTCCAGCGCCTGCTTTCCAATCTGACCACTCTGAGCATCTGGACCAGTGGCCAAGGCCTGG GCCACTCTGGCCACGTGCTCTTGTGTGAAGTCCATCTCACATCGGCCCGGCCTCAGCGTGGGCTTGCCCC ACCAGCCTCTTGGGTGGAGACCTGCTTATGTCCCCAGGGATACACGGGCCAGTTCTGTGAATTCTGTGCT CTGGGATACAAAAGAGAAATACCTCATGGGGGTCCCTATACCAACTGCATCCCCTGCACCTGCAACCAGC ATGGAACCTGTGACCCCAACACAGGGATCTGCCTGTGTGGCCACCACACGGAGGGTCCATCCTGTGAGCG ATGCATGCCAGGTTTCTACGGTAACGCCTTCTCAGGCCACGCTGATGATTGCCAGCCCTGTCCATGCCCT GGCCAATCAGCCTGCACGACCATCCCAGAGAGTAGAGATGTGGTGTGCACACACTGCCCCCCTGGTCAGA GAGGACGACGCTGTGAGAGCTGTGAAGATGGCTTCTTCGGAGATCCCCTAGGACTCTCTGGAGCTCCCCA GCCCTGCCGCCGATGCCAGTGCAGCAGGAACGTGGATCTCAATGCTGTGGGCAACTGCGATCCTCATTCT GGCCGCTGTCTGCGCTGTCTGCACAACACAACAGGGGCCCACTGCGAGCACTGTCAGGAGGGTTTCTATG GGAGTGCCTTGGCCACGAGGCCTGCGGACAAATGTGCTCCCTGCAGCTGTGACCCGAGGGGCTCAAGCAG TCAGAAGACCTGCAACCCAGCGACTGGCCAGTGTGCCTGCCTGCCTTACGTCACCGGGAGGGATTGCAGC CGCTGCAGCCCTGGCTTCTACGACCTCCAGCCTGGGAGGGGCTGCCAGAGCTGCAAATGTCACCCAGTGG GCTCCTTGGAGAACAAGTGCCACCCCAAGACTGGCCAGTGCCCCTGTCGACCCGGTGTCACCGGCCAAGC CTGTGACAGATGCCAGCTGGGTTTCTTTGGCTTCTCCATCAAGGGCTGCCGAGACTGTAGGTGTTCCCCA CTGGGTGCTGCCTCACCCCAGTGCCATGAGAACAGCACGTGTGTGTGCCGGCCTGGCTTCGTGGGCTATA AATGTGACCGCTGCCAGGACAATTTCTTCCTCGTGGATGGTGACACAGGCTGCCAAGAGTGCCCCACCTG CTATGCTCTAGTGAAGGAGGAGGCAGCCAAGCTGAAGACCAGGCTGATGCTGATGGAGGGGTGGCTTCAG GGGTCCGAGTGTGGCAGCCCCTGGGGACCACTAGACATTCTGCAGGGAGAAGCCCCTCGGGGGGATGTCT ACCAAGGTCACCACCTATTTCAAGAGGCCCGGGGGACCTTCCTGGAGCAGATGGTGGGCCTGGAGGAGTC TGTGAAGGCCACTTGGGAGCAGCTGCAGGTGCTGAGAGGGAACGCACACTGTGCCCAGGCTGGAGCTCAG AAGACCTGCAACCAGCTGGCAGAGCTGGGGGAGACGCTGCGTTCCTCAGAGGAGGAGGTCCTGCGTGCAG CCTCAGCTCTCTCATTTCTGGCAAATCTTCAGGAAGGATCCAGCGCACCCACCAATTGGGGTCGCCTGGC ATCAGAGGCCTACATCCTCACCAGGAGCCACAGGGACACAGCCACCAAGATCGAAGCTACCGTGGAAAGG GCCCTGCTCGCCTCCAACACCAGCTATGAGCTCCTGTGGAAGCTGTTGAAAGGCAGTGTGGCCTCAGAGG CCCAGCAGGAACTGGAGGAGAGGTACCAGGAGGTGCAGGCAGCTCAGACTGCCCTGGGCATAGCTGTGGC AGAGGCGCTGCCCAAAGCTGAAAAGGCACTAGCCACGGTGAAGCAAGTCGTTGGTGCCGCAGCCCCACGT CTGGGCTCGATGGTCACCCCTGAAGCAATGGACTCCCAAGCTAGATACCTAGGCCGGAGAGTGAAAGCCC TGGAGCAGAAGCTGCAGCAGAACCAGGCAGGCCAAGCGGTGGGAGCCCTGCAGGTAGAGGCTGGGAGAGC CTTGAGGAAGGTGGAGCCCTTTCTGCAGCTACGAAACAAGACCACAGCTGCCCTGACACAGGTTTCCTCA GCTGTCCAAGCTGCTAAGGTGACCGTCATAGGAGCGAAGACCCTGCTAGCTGACCTAGAGGGAATGAAGC TAAGGTTCCCTCTACCCAAGGAGCAGGCAGCGCTGAAGAGGAAAGCAGGCAGCATCAGGACCAGGCTCTT GAAGGACACGAAGAGAAAGACCAAGCAGGCAGAGAGGATGCTGGGAAATGCTGCCTCGCTCTCCTCCAAC TCCCAGAAGAGAAGCAAAGAAGCAGAACTGATGTCCAAGGAGAATGCCAAGCTTGCCAGAGCTTTCCTGA GGGAAGGGAAGCAGAACTACCGTCATGCCAGCCGACTCGCCAGCCAGACCCAGGCCACACTCCGCCAGGC CTCTCGCCTGGTGCTGACCTCAGAAGCACGGAAACAGGAGCTGGAAGAAGCTAAACAGGTGGCCTCTGGG CTGAGCACTGTGGAGCGCCAGATCCGAGAGTCTCGGATCTCCTTGGAGAAGGACACCAAGGTCCTGTCAG AGCTGCTCGCGAAACTGGGGTCCCTGGGTACCCACCAAGCCCCTGCTCAGACCCTGAACGAGACCCAGCA GGCACTAGAAAGCTTGAGGCTGCAGCTGGATTCACATGGATCCTTGCATCACAAACTAAGGCAGCTGGAG GAAGAGTCTGCTCGACAGGAGCTGCAGATCCAGAGCTTTGAGAACGACCTCGCTGAGATCCGCGCTGACA AGCACAACTTGGAGACCATTCTAAGCAGTCTACCAGAGAACTGTGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210588 representing NM_001107830 Red=Cloning site Green=Tags(s) MAVSGILSRLATVVCVALVMLKAHCATGAGMGSCYDGAGRAQRCLPEFENAAFGRRAEASHTCGQPPEDF CPHVGAPGAGPQCQRCDDADPRRRHDASYLTDFHSPDDSTWWQSPSMAFGVQYPTSVNLTLHLGKAYEIT YVRLKFHTSRPESFAIYKRTHTGGPWEPYQYYSASCQKTYGHPEGHYLRPGEDERVAFCTSEFSDISPLN GGNVAFSTLEGRPSAYNFEESPVLQEWVTSTDLLISLDRLNTFGDDIFKDPRVLQSYYYAVSDFSVGGRC KCNGHASACGPNEAGQLACHCQHNTTGVDCERCLPFFQDRPWARGTAEDANECLPCNCSGHSEECTFDRE LYRSTGHGGHCQRCRDHTAGPHCEHCEKNYYRWDPKTPCQPCDCHPAGSLSLQCDNSGTCPCKLTVTGWK CDRCLPGFHSLSEGGCRPCTCNVAGSLGTCDPRSGNCPCKENVEGGLCDRCRPGTFNLQPHNPAGCSSCF CYGHSKVCAPASGFQEHHIRSDFRHGADGWQARSTEVSERPLQWSQSGILLGLRGGEELSAPEKFLGDQR LSYGQPLILTLQVPPGGSPPPMQLRLEGAGLSLALKPSNLPSSQDTRQPGRVQLQFLLQETSEEAEPPLP AFHFQRLLSNLTTLSIWTSGQGLGHSGHVLLCEVHLTSARPQRGLAPPASWVETCLCPQGYTGQFCEFCA LGYKREIPHGGPYTNCIPCTCNQHGTCDPNTGICLCGHHTEGPSCERCMPGFYGNAFSGHADDCQPCPCP GQSACTTIPESRDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQPCRRCQCSRNVDLNAVGNCDPHS GRCLRCLHNTTGAHCEHCQEGFYGSALATRPADKCAPCSCDPRGSSSQKTCNPATGQCACLPYVTGRDCS RCSPGFYDLQPGRGCQSCKCHPVGSLENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKGCRDCRCSP LGAASPQCHENSTCVCRPGFVGYKCDRCQDNFFLVDGDTGCQECPTCYALVKEEAAKLKTRLMLMEGWLQ GSECGSPWGPLDILQGEAPRGDVYQGHHLFQEARGTFLEQMVGLEESVKATWEQLQVLRGNAHCAQAGAQ KTCNQLAELGETLRSSEEEVLRAASALSFLANLQEGSSAPTNWGRLASEAYILTRSHRDTATKIEATVER ALLASNTSYELLWKLLKGSVASEAQQELEERYQEVQAAQTALGIAVAEALPKAEKALATVKQVVGAAAPR LGSMVTPEAMDSQARYLGRRVKALEQKLQQNQAGQAVGALQVEAGRALRKVEPFLQLRNKTTAALTQVSS AVQAAKVTVIGAKTLLADLEGMKLRFPLPKEQAALKRKAGSIRTRLLKDTKRKTKQAERMLGNAASLSSN SQKRSKEAELMSKENAKLARAFLREGKQNYRHASRLASQTQATLRQASRLVLTSEARKQELEEAKQVASG LSTVERQIRESRISLEKDTKVLSELLAKLGSLGTHQAPAQTLNETQQALESLRLQLDSHGSLHHKLRQLE EESARQELQIQSFENDLAEIRADKHNLETILSSLPENCAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107830
ORF Size	4740 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107830.1, NP_001101300.1
RefSeq Size	6512 bp
RefSeq ORF	4743 bp
Locus ID	311862
MW	172 kDa

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