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Trim72 (NM_001077675) Rat Tagged ORF Clone
CAT＃: RR210882
- TrueORF®
Trim72 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 72 (Trim72), (10 ug)
"NM_001077675" in other vectors (3)
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CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1562778 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210882 representing NM_001077675
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGCACCAGGCCTTTTGCGCCAGGAACTGTCTTGCCCGCTGTGCTTGCAGCTGTTCGATGCAC CAGTGACCGCTGAGTGTGGCCACAGTTTCTGCCGTGCCTGCCTGATCCGTGTGGCAGGGGAGCCTGCCGA CGATGGCACGGTTGCCTGTCCCTGTTGTCAGGCATCTACTCGGCCACAGGCGCTAAGCACTAACCTCCAG TTGGCACGCCTTGTGGAGGGTTTGGCACAAGTGCCCCAAGGCCACTGCGAGGAACACCTGGATCCACTGA GCATCTACTGCGAGCAGGACCGCACACTTGTGTGTGGTGTGTGTGCCTCTCTCGGTTCACACCGTGGTCA CCGTCTTCTGCCTGCCGCAGAAGCCCATGCACGTCTCAAGACACAGCTTCCACAACAGAAGGCCCAGCTG CAGGAGGCATGCATGCGCAAGGAGAAGAGTGTAGCAGTCCTGGAGCATCAGCTGGTGGAGGTGGAGGAGA CCGTGCGTCAGTTCCGGGGAGCTGTTGGGGAGCAGCTGGGGAAGATGCGGATGTTCCTGGCTGCCCTAGA AAGTTCTTTGGACCGTGAAGCAGAAAGGGTTCGAGGTGAGGCAGGGGTTGCCTTGCGGCGGGAGCTGTCA AGCCTGAACTCTTACCTGGAGCAACTGAGGCAGATGGAGAAGGTGCTGGAGGAGGTGGCTGACAAGCCAC AGACAGAATTCCTCATGAAATTCTGCCTGGTGACCAGCAGGCTGCAGAAGATTCTGTCAGAGTCACCACC CCCAGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAGAAGATG TTCCGGGCTCTGATGCCAGAGTTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCCTGGTGG TGTCCGCCTCTGGTCGCCGAGTGGAGTGCTCGGAGCAGAAGGCGCCGCCAGCAGGAGAAGACACGTGCCA GTTCGACAAGACGGTAGCGGTAGTGGCGAAGCAGCTGCTGTCACAGGGGGAGCACTACTGGGAGGTGGAG GTGGGCGACAAGCCACGCTGGGCCCTGGGTGTGATGGCGGCTGACGCTTCCCGTCGTGGCCGCCTGCACG CGGTGCCCTCACAGGGGCTGTGGTTGCTCGGCCTGCGCGATGGCAAGATCCTGGAGGCACACGTGGAGGC CAAGGAGCCACGGGCACTGCGCACCCCAGAGAGGCCACCAGCGCGCATTGGCCTCTACCTAAGCTTTGCA GATGGCGTCCTGACTTTCTATGATGCAAGCAACACCGACGCGCTTACACCGCTCTTTTCTTTTCATGAGC GTCTGCCTGGGCCGGTGTACCCCATGTTTGACGTGTGCTGGCACGACAAGGGCAAGAATTCTCAGCCGCT GTTGCTCGTGGGGCCAGACAGTGAGCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210882 representing NM_001077675
Red=Cloning site Green=Tags(s) MSTAPGLLRQELSCPLCLQLFDAPVTAECGHSFCRACLIRVAGEPADDGTVACPCCQASTRPQALSTNLQ LARLVEGLAQVPQGHCEEHLDPLSIYCEQDRTLVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKAQL QEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRMFLAALESSLDREAERVRGEAGVALRRELS SLNSYLEQLRQMEKVLEEVADKPQTEFLMKFCLVTSRLQKILSESPPPARLDIQLPVISDDFKFQVWKKM FRALMPELEELTFDPSSAHPSLVVSASGRRVECSEQKAPPAGEDTCQFDKTVAVVAKQLLSQGEHYWEVE VGDKPRWALGVMAADASRRGRLHAVPSQGLWLLGLRDGKILEAHVEAKEPRALRTPERPPARIGLYLSFA DGVLTFYDASNTDALTPLFSFHERLPGPVYPMFDVCWHDKGKNSQPLLLVGPDSEQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077675 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077675.1, NP_001071143.1 |
| RefSeq Size | 2363 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 365377 |
| UniProt ID | A0JPQ4 |
| MW | 52.8 kDa |
| Gene Summary | Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210882 | Trim72 (untagged ORF) - Rat tripartite motif-containing 72 (Trim72), (10 ug) |
CNY 6,180.00 |
|
| RR210882L3 | Lenti ORF clone of Trim72 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 72 (Trim72), (10 ug) |
CNY 7,980.00 |
|
| RR210882L4 | Lenti ORF clone of Trim72 (mGFP-tagged ORF) - Rat tripartite motif-containing 72 (Trim72), (10 ug) |
CNY 8,840.00 |
