United States
Germany
Japan
United Kingdom
China
Amph (NM_022217) Rat Tagged ORF Clone
CAT＃: RR210952
- TrueORF®
Amph (Myc-DDK-tagged ORF) - Rat amphiphysin (Amph), (10 ug)
"NM_022217" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Amph1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210952 representing NM_022217
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACATCAAGACGGGCATCTTCGCCAAAAACGTGCAAAAGCGGCTCAACCGGGCACAGGAAAAGG TCCTCCAGAAGCTGGGAAAGGCAGATGAAACAAAAGATGAACAGTTTGAAGAATATGTTCAGAACTTCAA GCGGCAAGAGGCAGAGGGTACCAGACTTCAGCGAGAACTGCGAGGATATTTAGCGGCAATCAAAGGTATG CAGGAGGCCTCAATGAAGCTTACCGAGTCACTACATGAAGTCTACGAGCCAGATTGGTATGGACGGGAAG ATGTAAAGATGGTTGGTGAGAAGTGTGATGTGCTGTGGGAAGACTTCCATCAAAAGCTAGTGGATGGGTC CTTGCTGACACTGGACACGTACCTGGGCCAGTTTCCTGACATAAAGAATCGCATCGCTAAGCGCAGCAGG AAGCTCGTGGATTACGACAGTGCCCGCCATCACCTGGAGGCTCTGCAGAGTTCCAAAAGGAAGGATGAGA GTCGGATCTCTAAGGCAGAAGAGGAGTTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTCAATGTTGACTT ACAAGAAGAATTGCCATCACTATGGTCAAGACGAGTTGGGTTTTATGTCAATACTTTCAAAAACGTCTCT AGCCTTGAGGCCAAGTTTCACAAGGAAATCGCAGTGCTTTGCCACAAGCTTTATGAAGTGATGACGAAAC TTGGTGATCAGCACGCTGACAAGGCCTTCAGCATTCAAGGAGCTCCCAGTGATTCGGGTCCTCTTCGCAT TGCAAAGACACCATCACCCCCAGAGGAGGCTTCACCCCTCCCCAGCCCAACGGCTAGTCCCAACCACACA TTAGCACCTGCATCTCCTGCCCCAGTGCGACCCAGATCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC CACCTCTGCCTAAAGTCACCCCAACAAAGGAACTACAGCAGGAGAACATCATCAATTTCTTTGAGGACAA CTTTGTACCAGAAATCAATGTGACAACACCTTCCCAGAATGAAGTCCTGGAGGTGAAGAAGGAGGAGACT TTGCTGGATTTGGACTTTGACCCTTTCAAACCTGATGTGACCCCAGCAGGTTCTGCTGCAGCGACCCACT CACCCATGTCTCAGACATTGCCCTGGGACTTATGGACGACAAGCACTGATTTGGTACAACCGGCTTCTGG AGGTTCGTTTAATGATTTTACCCAGCCCCAGGATACTTCATTATTCACGATGCAGACAGATCAGAATATG GCTGAAACTGAGCAGGCTCTACCTACAGAGCCCCAGGCAGAGGAGCCTCCCACCACTGCTGCTGCACCTA CTGCTGGGCTGGACCTTGGGCTAGAGATGGAGGAGCCAAAGGAGGAGGCAGCGATCCCTCCTGGAACTGA TGCTGGTGAGACTGTGGGAACTGAGGGGTCCACAGGGGAGGAAGCAGAGGCAGAGAAGGCAGCCCTACCT GCTGGAGAAGGCGAAAGTCCTGAGGGAGCCAAAATCGATGTGGAAAGCACTGAGCTTGCAAGCAGTGAGA GCCCTCAAGCAGCAGAGCTTGAAGCTGGTGCTCCTCAGGAAAAGGTCATCCCTTCTGTTGTCATAGAGCC AGCCTCCAACCATGAAGGAGAAGAACACCAAGAAACCACTACGGGTACTGAAACAAGGGAGGCCACTGAG GACGTGGCTCCCCAGGGCCCAGCCGGTGAGAAGCAGGAACTGGCTACAGAGCCCACGCCTCTGGATTCTC AGGCTGCTACGCCTGCACCAGCAGGTGCTGTGGATGCTTCGCTGTCTGCAGGGGATGCCGCTCAGGAACT GCCTCCTGGCTTTCTCTACAAGGTGGAAACTCTGCATGACTTCGAGGCAGCGAACTCTGATGAACTTACC CTACAAAGGGGTGATGTGGTGCTCGTGGTGCCCTCAGACTCAGAAGCCGACCAGGATGCAGGCTGGTTGG TGGGGGTGAAGGAGTCAGACTGGCTTCAGTACAGAGACCTGGCCACATACAAAGGCCTCTTTCCAGAGAA CTTCACACGACACCTAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210952 representing NM_022217
Red=Cloning site Green=Tags(s) MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGM QEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSR KLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVS SLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEASPLPSPTASPNHT LAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELQQENIINFFEDNFVPEINVTTPSQNEVLEVKKEET LLDLDFDPFKPDVTPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQPQDTSLFTMQTDQNM AETEQALPTEPQAEEPPTTAAAPTAGLDLGLEMEEPKEEAAIPPGTDAGETVGTEGSTGEEAEAEKAALP AGEGESPEGAKIDVESTELASSESPQAAELEAGAPQEKVIPSVVIEPASNHEGEEHQETTTGTETREATE DVAPQGPAGEKQELATEPTPLDSQAATPAPAGAVDASLSAGDAAQELPPGFLYKVETLHDFEAANSDELT LQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRHLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022217 |
| ORF Size | 2049 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022217.1, NP_071553.1 |
| RefSeq Size | 2052 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 60668 |
| UniProt ID | O08838 |
| MW | 74.9 kDa |
| Gene Summary | activates dynamin GTPase activity; may play a role in clatherin mediated endocytosis in synaptic vesicles [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210952 | Amph (untagged ORF) - Rat amphiphysin (Amph), (10 ug) |
CNY 8,840.00 |
|
| RR210952L3 | Lenti ORF clone of Amph (Myc-DDK-tagged ORF) - Rat amphiphysin (Amph), (10 ug) |
CNY 10,550.00 |
|
| RR210952L4 | Lenti ORF clone of Amph (mGFP-tagged ORF) - Rat amphiphysin (Amph), (10 ug) |
CNY 11,690.00 |
