Cadps (NM_013219) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Caps; Caps1; Caps2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211146 representing NM_013219 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACCCTTCGTCCAGCGAAGAGGAATCGGATGAGATCCTGGAAGAGGAGAGCGGCAAGGAGGTGC TGGGCTCGGCCGCGTCCGGAGCGCGCCTGTCTCCAAGCCGCACCAACGAGGGCTCGGCGGGCAGCGCCGG CATGGGGGGCAGTGGCGCCGGGGCCGGGGTGGGCGCCGGCGGCGGTGGGGGTAGCGGCGCGAGCAGCGGC GGCGGGGCCGGGGGGCTGCAGCCCAGCAGCCGCGCCGGCGGCGGCCGGCCCTCCAGCCCCAGCCCGTCGG TGGTGAGCGAGAAGGAGAAGGAAGAGTTGGAGCGGCTGCAGAAGGAAGAGGAGGAGAGGAAGAAGAGGCT GCAGCTGTACGTGTTCGTGATGCGCTGCATTGCCTACCCCTTCAACGCCAAGCAGCCCACCGACATGGCT CGGCGGCCAAGGAAGATCAGCAAACAGCAGCTGCAGACTGTCAAGGACCGATTCCAGGCTTTCCTCAATG GGGAGACCCAAATCGTGGCAGATGAAGCCTTTATGAATGCTGTCCAGAGCTACTATGAGGTGTTTCTCAA GAGTGACCGTGTGGCCCGCATGGTACAGAGTGGGGGCTGCTCAGCCAACGACTCTCGGGAGGTCTTCAAG AAACACATCGAGAAGAGGGTACGCAGCCTACCTGAGATTGATGGCCTCAGCAAGGAGACTGTGCTAAGCT CCTGGATGGCTAAGTTTGACGCCATCTACCGTGGTGAGGAGGACCCCAGGAAGCAGCAAGCCCGGATGAC AGCCAGTGCAGCTTCTGAGTTGATTCTGAGCAAGGAACAGCTCTATGAGATGTTCCAGAACATTCTTGGG ATCAAGAAATTTGAACATCAACTCCTATATAATGCGTGTCAGCTGGACAATCCAGATGAGCAGGCAGCCC AGATCAGACGAGAGCTGGACGGACGTCTCCAAATGGCAGACCAAATAGCCAGGGAACGCAAATTTCCTAA GTTCGTATCCAAAGAAATGGAAAATATGTACATCGAGGAGCTGAAGTCCTCCGTCAACCTACTCATGGCC AACTTGGAGAGCATGCCGGTGTCCAAGGGTGGAGAGTTCAAGCTGCAGAAACTGAAACGCAGTCACAACG CGTCCATCATTGACATGGGTGAGGAGAGCGAGAACCAGCTGTCCAAGTCGGATGTCCTGCTGTCCTTCTC CTTGGAGGTTGTCATCATGGAAGTCCAAGGCCTTAAGTCCTTGGCTCCAAACCGAATCGTGTACTGCACA ATGGAGGTGGAGGGTGGAGAGAAACTTCAGACTGATCAAGCAGAGGCTTCTAAACCAACCTGGGGCACTC AAGGCGACTTCTCTACAACCCACGCGCTGCCAGCTGTTAAAGTAAAGCTATTCACAGAGAGCACTGGTGT CCTGGCCTTGGAAGACAAGGAACTCGGGCGGGTTATCCTCCATCCCACCCCAAACAGCCCCAAACAATCC GAGTGGCACAAAATGACAGTCTCCAAAAACTGCCCCGATCAAGACCTCAAAATCAAACTCGCTGTCCGAA TGGATAAGCCTCAAAACATGAAGCACTCTGGGTACTTATGGACCATTGGTAAGAATGTGTGGAAGAGGTG GAAGAAAAGGTTCTTTGTGTTGGTGCAGGTCAGCCAGTACACTTTTGCCATGTGCAGCTATCGAGAAAAG AAAGCAGAACCTCAGGAACTCCTACAGCTGGATGGCTACACCGTGGATTACACCGACCCTCAGCCAGGTT TGGAGGGTGGCCGAGCCTTCTTCAATGCAGTCAAAGAAGGAGACACGGTGATATTTGCCAGTGACGATGA GCAAGATCGAATCCTGTGGGTCCAAGCTATGTACAGGGCCACCGGGCAGTCGCACAAACCTGTGCCCCCT ACCCAGGTGCAGAAGCTCAAGCCAAGGGCGGAAACGTGCCTCAGCATGGATGCCCCCATCTCCCAGTTTT ACGCAGATAGAGCTCAAAAGCATGGCATGGATGAATTTATCTCGTCCAACCCCTGTAACTTTGACCACGC TTCCCTCTTTGAGATGGTACAGCGGCTGACTTTGGATCACAGACTAAACGATTCCTATTCTTGCCTGGGC TGGTTCAGTCCTGGCCAGGTGTTTGTGTTAGACGAGTACTGCGCTCGGAATGGAGTCCGAGGATGTCATC GGCATCTCTGTTACCTTAGAGACTTGCTGGAACGGGCAGAAAATGGCGCCATGATCGACCCCACGCTTCT TCATTACAGCTTTGCCTTCTGCGCATCCCATGTCCACGGGAACAGGCCTGATGGAATCGGAACTGTGACT GTTGAAGAAAAAGAGCGCTTTGAAGAAATCAAAGAGAGACTCCGAGTTCTGCTGGAAAATCAAATTACAC ATTTCAGGTACTGCTTTCCGTTTGGTCGACCTGAAGGTGCTTTGAAAGCGACTCTCTCACTTTTGGAACG GGTTTTGATGAAAGATATTGTCACTCCAGTACCCCAAGAGGAGGTCAAAACTGTCATCCGTAAATGCCTA GAGCAGGCTGCTCTAGTCAACTACTCTCGCCTATCTGAGTATGCCAAAATCGAAGAAAATGTAGGCCGCT TAATCACTCCCGCCAAAAAGCTCGAAGACACAATCCGTCTTGCTGAACTAGTCATTGAAGTGCTGCAACA AAACGAAGAGCACCACGCCGAGGCCTTTGCGTGGTGGTCAGACTTAATGGTGGAGCACGCGGAGACGTTC CTGTCACTCTTTGCAGTGGACATGGATGCAGCATTAGAGGTGCAACCCCCAGACACGTGGGACAGTTTCC CACTCTTCCAGCTGCTGAATGACTTTCTCCGTACAGACTATAACTTGTGCAATGGAAAGTTTCACAAACA CCTGCAGGATCTGTTCGCCCCACTCGTTGTTAGATACGTGGATCTGATGGAGTCCTCAATTGCACAATCT ATCCACAGGGGCTTTGAGCGGGAGTCATGGGAACCAGTCAATAACGGATCGGGTACATCAGAAGATTTGT TCTGGAAACTTGATGCCCTTCAGACTTTCATCCGTGACCTACACTGGCCTGAGGAAGAGTTTGGAAAGCA CTTAGAGCAGCGGCTAAAACTAATGGCAAGCGACATGATTGAGTCCTGCGTCAAGAGAACCAGGATTGCA TTTGAAGTTAAGCTGCAAAAAACCAGTCGATCAACAGATTTTCGAGTCCCACAGTCAATATGCACCATGT TTAATGTTATGGTTGATGCCAAAGCTCAATCAACAAAACTTTGCAGCATGGAAATGGGCCAGGAGCATCA ATACCATTCAAAAATAGACGAACTAATTGAAGAAACCGTTAAGGAAATGATCACACTCTTGGTGGCAAAG TTTGTAACTATATTGGAAGGCGTACTGGCAAAGTTATCCAGATACGACGAGGGGACTTTGTTTTCTTCGT TTCTGTCATTTACGGTGAAGGCGGCATCTAAGTATGTGGACGTACCTAAACCCGGGATGGATGTGGCCGA CGCCTACGTGACTTTCGTCCGCCACTCTCAGGATGTCCTTCGTGATAAGGTCAATGAGGAGATGTATATA GAAAGGTTATTTGATCAATGGTACAACAGCTCCATGAACGTCATCTGCACCTGGCTGACCGACCGGATGG ACCTCCAGCTTCACATTTATCAGCTGAAAACACTAATTAGGATGGTAAAGAAAACCTACAGAGATTTCCG ATTGCAAGGGGTCCTGGATTCCACCTTAAACAGCAAGACCTATGAGACCATTCGAAATCGGCTCACTGTA GAGGAACGTACCGCGTCCGTGAGTGAGGGAGGGGGCTTGCAAGGCATCAGCATGAAGGACAGTGACGAGG AAGATGAAGAAGACGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211146 representing NM_013219 Red=Cloning site Green=Tags(s) MLDPSSSEEESDEILEEESGKEVLGSAASGARLSPSRTNEGSAGSAGMGGSGAGAGVGAGGGGGSGASSG GGAGGLQPSSRAGGGRPSSPSPSVVSEKEKEELERLQKEEEERKKRLQLYVFVMRCIAYPFNAKQPTDMA RRPRKISKQQLQTVKDRFQAFLNGETQIVADEAFMNAVQSYYEVFLKSDRVARMVQSGGCSANDSREVFK KHIEKRVRSLPEIDGLSKETVLSSWMAKFDAIYRGEEDPRKQQARMTASAASELILSKEQLYEMFQNILG IKKFEHQLLYNACQLDNPDEQAAQIRRELDGRLQMADQIARERKFPKFVSKEMENMYIEELKSSVNLLMA NLESMPVSKGGEFKLQKLKRSHNASIIDMGEESENQLSKSDVLLSFSLEVVIMEVQGLKSLAPNRIVYCT MEVEGGEKLQTDQAEASKPTWGTQGDFSTTHALPAVKVKLFTESTGVLALEDKELGRVILHPTPNSPKQS EWHKMTVSKNCPDQDLKIKLAVRMDKPQNMKHSGYLWTIGKNVWKRWKKRFFVLVQVSQYTFAMCSYREK KAEPQELLQLDGYTVDYTDPQPGLEGGRAFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSHKPVPP TQVQKLKPRAETCLSMDAPISQFYADRAQKHGMDEFISSNPCNFDHASLFEMVQRLTLDHRLNDSYSCLG WFSPGQVFVLDEYCARNGVRGCHRHLCYLRDLLERAENGAMIDPTLLHYSFAFCASHVHGNRPDGIGTVT VEEKERFEEIKERLRVLLENQITHFRYCFPFGRPEGALKATLSLLERVLMKDIVTPVPQEEVKTVIRKCL EQAALVNYSRLSEYAKIEENVGRLITPAKKLEDTIRLAELVIEVLQQNEEHHAEAFAWWSDLMVEHAETF LSLFAVDMDAALEVQPPDTWDSFPLFQLLNDFLRTDYNLCNGKFHKHLQDLFAPLVVRYVDLMESSIAQS IHRGFERESWEPVNNGSGTSEDLFWKLDALQTFIRDLHWPEEEFGKHLEQRLKLMASDMIESCVKRTRIA FEVKLQKTSRSTDFRVPQSICTMFNVMVDAKAQSTKLCSMEMGQEHQYHSKIDELIEETVKEMITLLVAK FVTILEGVLAKLSRYDEGTLFSSFLSFTVKAASKYVDVPKPGMDVADAYVTFVRHSQDVLRDKVNEEMYI ERLFDQWYNSSMNVICTWLTDRMDLQLHIYQLKTLIRMVKKTYRDFRLQGVLDSTLNSKTYETIRNRLTV EERTASVSEGGGLQGISMKDSDEEDEEDD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013219
ORF Size	3867 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013219.1, NP_037351.1
RefSeq Size	4926 bp
RefSeq ORF	3870 bp
Locus ID	26989
UniProt ID	Q62717
MW	146.3 kDa
Gene Summary	plays a role in calcium ion-dependent regulated exocytosis in neuroendocrine cells [RGD, Feb 2006]

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