Adamtsl3 (NM_001107533) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211596 representing NM_001107533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGAGGGAGACTCCTAGAATATTGGACCCCATGGCTTTCTGGACAGGATCCTGGTGGCTGCTGA CACGAATGATCTTTACAGTCTCTGGTCTCCTGCAGGCCACAGCAGAGAAGTCTCCTGGAGCCTACTTCCT TCCTGAGTTCGCACTCTCTCCTCAGGGAAGCTTTCTAGAAGATACCACAGGGGAACAGTTCCTCACATAC CGCTATGATGATCAGACCTCAAGAAATGCTCACTCCGATGAAGACAAAGATGGCAACTGGGATGCCTGGG GAGACTGGAGTGACTGCTCCCGGACCTGTGGGGGAGGAGCCTCCTATTCTTTGCGAAGATGTCTGACTGG AAGGAACTGTGAAGGACAGAACATCAGATACAAGACTTGCAGCAATCACGACTGCCCAGCAGATGCAGAA GATTTCAGAGCCCAGCAGTGCTCAGCCTACAATGATGTGCAGTACCAGGGTCATTATTATGAATGGATTC CACTACACAATGACCCTGCTGCCCCATGTGCACTCAAGTGTCATGCTCGGGGACAGAGTTTGGTGGTAGA GCTGGCACCCAAAGTGCTGGATGGAACTCGTTGCAATGCTGACACTCTGGATATGTGCATAAGTGGCATC TGCCAGACTGTGGGCTGCGATAGACAGCTGGGTAGTAGCGCCAAGAAGGACAACTGTGGGGTCTGTGCAG GGGACGGCTCCACCTGCAGGCTGGTGCGGGGTCAGACCAAGGTGCACCTGTCTCCTGAGAAAAAGGAAGA AAATGTGATTGCTGTTCCCTTGGGCAGTCGAAGTGTGAGGATTACTGTGAAAGGACCTGCCCTGCTCTTC ATTGAGTCAAAAACACTTCAAGGAAGCAGAGGAGAACACAGCTTCAACAGCCCTGGGGTTTTTGTTGTGG AAAACACGACGATTGAGTTTCAGAAAGGTGCCGACAGACAAACCTTCAAGATTGCGGGACCCCTGATGGC TGACTTCATCTTCAAGACCAGGTACACGGTGGCCAAAGGCAGCGTGGTCCAGTTCTTCTTTTACCAGCCC ATCAGCCATCAGTGGAGACAGACTGACTTCTTTCCCTGCACGGTGACATGTGGAGGAGGTTATCAGCTCA ACTCTGCTGAGTGCATGGACATCCGCTTGAAAAGGGTAGTGCCTGACCACTATTGCCATTACTACCCAGA AAATGTGAAGCCCAAACCAAAGCTGAAGGAATGCAGCATGGATCCTTGTCCATCAAGTGATGGATTTAAA GAGATTATGCCCTATGACCACTTCCAACCTCTCCCTCGCTGGGAACATAATCCTTGGACTGCATGTTCGG TGTCCTGTGGAGGAGGGATTCAGAGACGGAGTTTCGTGTGCGTAGAGGAATCTATGCATGCAGAGATTCT CCAAGTGGAAGAATGGAAGTGCATGTATGCACCCAAGCCCAAGGTGATGCAGACTTGCAACCTGTTTGAT TGCCCCAAGTGGATTGCCATGGAGTGGTCTCAGTGCACAGTGACTTGTGGCCGAGGGTTGCGCTACCGAG TGGTTCTGTGTATCAACCATCGTGGGCAGCATGTTGGGGGCTGCAACCCACAGATGAAGCTGCACATCAA AGAGGAGTGTGTCATCCCCGTCCCCTGCTATAAGCCTAAAGAAAAAAGTCCAGTGGAGGCTAAATTACCT TGGCTGAAACAAGCACAAGAGCTAGAAGAGACCAGAATAGCCACAGAAGAACCGAAGTTCATCCCAGAGC CCTGGTCGGCCTGCAGCACTACATGTGGGCCCGGCATTCAGGTGCGTGAGGTGAGGTGTCGAGTGCTCCT CACATTCACACAGACAGAGACTGAGCTCCCTGAGGAGGAATGTGAAGGCCCCAAGCCACCCACAGAGCGA CCCTGCCTCCTTGAAGCCTGTGACCACAGCCCTGTGTCTCGAGAACTGGGTGGCCCTCTCCAGGAGAAGG ACAGCGAGATGATTTACGACTGGGAGTATGCTGGCTTTACCCCCTGCACGGCAACATGTTTGGGAGGCTA CCAAGAAGCCATAGCTGTGTGCTTACACATCCAGACCCATCAGACCGTCAATGACAGCCTGTGTGACCTA GCTCACCGTCCTCCACCAATGAGTCAGGCCTGTCACACGGAGCCCTGCCCACCCAGATGGCACATGGGTT CTTGGGGGGCCTGTTCAGCCACCTGTGGTGTTGGAATCCAGACGAGAGATGTATACTGCGTACATCCCGA GGAATCCCCAGCCCCGCCGGAGGAATGCAGAGATGAGAAGCCTCATGCCCTACGAGCATGCAATCAGTTT GATTGCCCTCCCAGCTGGCATATCGAAGAGTGGCAGCAGTGTCCCAGGACATGTGGTGGGGGAACTCAGA ACCGGAGAGTCACCTGCCGACAGCTGTTAACTGATGGCAGCTTTCTGAATTTGTCCGATGAACTGTGCCA AGGACCCAAGGCATCGTCTCATAAGTCCTGTGCCAGGACAGACTGCCCCCCACAGCTGGCTGCTGGAGAC TGGTCGAAGTGTTCTGTCACCTGTGGGGTTGGATTCCAGAGAAGAAAGCAGGTGTGTCAAAAGCTGACTG CCAAAGGCCGAAGAGTCCCCCTAGCTGAGACGCTGTGCAGGGACCTGCCGGGGCTCCCCCTCGTGAGACC TTGCCAGATGCCGGTGTGCAGCAAACTGAAGCTGGGAATGAAGACAAACCTTGGAGAGCGGGGCCCCCAG ATTCTGGGTGTCCGGAGAGTCTATATCCAGACAAGGGAGGAGAAGCGGATCAACCTGACGGTCGGCAGTA GAGCCTACTTGCTGCTCAATACATCTGTGATCATCAAATGCCCAGTACGACATTTCCAGAAGTCTCTAAT CCAGTGGGAGAAGGATGGCCATTGTCTGAAGAACTCTAAGCAGCTTGGCATCACCAAGTCAGGTTCACTG AAAATCCACAGTCTTTCCACTCCTGACATTGGCGTGTATAGATGCATTGCAGGTTCTGCACAGGAAACGG TTGTTCTCAAGCTCATTGGGACTGACAACCGGCTCATTGCTCACCCGTCCCTACGAGAGCATGCGCGAGC TTATCCTGGGTTGGACCATAATGAAGCCAATAGCTTGGGAGCCACATGGCATAAAATGCGCCAGTTGTGG AATAGCAAAAATGACCTTTATCTGCATGGTGGCGAAATCAATAAGCAGCCTTTCTTAAGAGCTCTGTTCG GGCCGTGCAGGACCTCCGAAGGAAATGATAACTCCTGGGAGTTTAAGAGCAAGCAGTTTGAAGCAGCCAT GAAACAGGGAGCATACAGCATGGACACAGCCCAGTTTGATGAGCTTATAAGAAACATGAGCCAGCTTATG GAGACGGGGGAGGCCAGCGACGACCTGGCATCCCAGATGATATATCAGCTGGTAGCTGAATTGGCCAAGG CTCAGCCTGTGCAGTGGAGGGGCACCCACGAGGAAGTTGTTGCATCTGAGGCTCAATTCAGAGGGGAGAC AGGAAGAGTTCCTAAAAGCCCCACAGTAAAAAACCCAGGCAAACTGACTTTTAAGCCAAAAGGACCTGTT CTCATGAGGCAAACCCTACCCCCTTCGGTTTCGTTTAACAAAACAATAAATGCGAGGATTGGGAATACAG TGTACATTACAAAAAGCACAGAGGTCATCAATATACTTTGCAGCCTCGTTACTCCCAGCAGTGAGGCCAC ATACACGTGGACCAAGGATGGAGCCTTGCTGCAGCCCTCTGTGAAGATCATTGTGGATGAAACTGGGAAG ATGCAGATACAGAATCCCACAAGGAAAGAGCAAGGGATATATGAATGTTCTGTAGCCAGTCATCAGGGAT CCGACGTGGAAAGCTCATTGGTGTTGTATGCAGAGGCACCTGTCATCTTGTCTATTGAAAGAAATGTCAC CAAACCAGAGCACAACCATCTGTCTGTTGTTGTGGGAGGCATTGTGGAGGCAGCCCTTCAAGCAAATGTG ACAATCCACTGTCCTGTAAAAGGTGTCCCTCAGCCTAATGTAACTTGGTTGAAGAGAGGAGGATCTCTGA GTGACAATGTTTCCTTGCTTTTCAATGGATCCCTGTTGTTGCAGAATGCTTCCCTTGAAAATGAAGGAAC CTACATTTGCACAGCCACCAATGCTCTTGGGAAAGCAGTGGCAACCTCTGGGCTCCGCTTGCTGGAACGA AGAGAACCGGGCAATAAGACTGCATTTCCTAAGGCACAGAAAAAGCGAGTGCTTCAGGCATCCAAAAACA GAGCAAACAGCAACAACTCAACAGGAAAGTCTCTGCCTCAAGAGTATTTTTGGGAACCTGGTAACTGGAC ACACTGTTCTGCCACCTGTGGACCTTTGGGAGTCCGGCTTCAGAGGCCCCAGTGCATGATGGCCAGTGGG CAGGAAGTGAGTGAAGCCCTGTGTGGTCCTCACAGGAAGCCGCTGGCTGGGTTTCAGTCCTGTAACATCC GGGATTGCCCAGCAAGGTGGTTTACGAGCATGTGGTCAGAGTGCTCTGTGTCTTGTGGTGGAGGATCTCA CAGTCGGCAAGTGATGTGCAAGCAGACAAAAGCCAATGGGACTGTGCAGGTGGTGTCCCCAAGGGCATGT GCTTCTAAAGACAGGCCTCTAGGGAGAAAACCGTGTTCTGTCCGTCCGTGTGCTCAACAGGCTACCGACC TAGGAAGCCAGTGTCCTGGACGTTGCATGGGCCAGGCCATGAGGATGCAGCACCACCACACGCCGTGTGC TCACAACAGCTCCAACTCTGACTGTGAGGACAGGAGGAGACTTGCCTTTAGAAGAAACTGCACCTCAGGG CCATGCGAGGCATGTTGGCGTGTGGGTCCCTGGAAGCCCTGCACAGCAGTCTGTGGCAGGGGCTTCCAGT CTCGCAAAGTCAACTGCATTCACACAGGGAGCTGCAAACCCATGGCTGACAGATACTGTGTGCAGATGAA ACCAGCTTCCTGGAGGCACTGTTTCGGGCCTTCCTGTGATAGAAACTGCATAGACACAACTCACTACTGT ATGTTCGTAAAGCACCTTAATCTGTGCTCCCTGGCCCTCTACAAACAAAGGTGCTGCCAGTCATGCCAAG AAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211596 representing NM_001107533 Red=Cloning site Green=Tags(s) MAVRETPRILDPMAFWTGSWWLLTRMIFTVSGLLQATAEKSPGAYFLPEFALSPQGSFLEDTTGEQFLTY RYDDQTSRNAHSDEDKDGNWDAWGDWSDCSRTCGGGASYSLRRCLTGRNCEGQNIRYKTCSNHDCPADAE DFRAQQCSAYNDVQYQGHYYEWIPLHNDPAAPCALKCHARGQSLVVELAPKVLDGTRCNADTLDMCISGI CQTVGCDRQLGSSAKKDNCGVCAGDGSTCRLVRGQTKVHLSPEKKEENVIAVPLGSRSVRITVKGPALLF IESKTLQGSRGEHSFNSPGVFVVENTTIEFQKGADRQTFKIAGPLMADFIFKTRYTVAKGSVVQFFFYQP ISHQWRQTDFFPCTVTCGGGYQLNSAECMDIRLKRVVPDHYCHYYPENVKPKPKLKECSMDPCPSSDGFK EIMPYDHFQPLPRWEHNPWTACSVSCGGGIQRRSFVCVEESMHAEILQVEEWKCMYAPKPKVMQTCNLFD CPKWIAMEWSQCTVTCGRGLRYRVVLCINHRGQHVGGCNPQMKLHIKEECVIPVPCYKPKEKSPVEAKLP WLKQAQELEETRIATEEPKFIPEPWSACSTTCGPGIQVREVRCRVLLTFTQTETELPEEECEGPKPPTER PCLLEACDHSPVSRELGGPLQEKDSEMIYDWEYAGFTPCTATCLGGYQEAIAVCLHIQTHQTVNDSLCDL AHRPPPMSQACHTEPCPPRWHMGSWGACSATCGVGIQTRDVYCVHPEESPAPPEECRDEKPHALRACNQF DCPPSWHIEEWQQCPRTCGGGTQNRRVTCRQLLTDGSFLNLSDELCQGPKASSHKSCARTDCPPQLAAGD WSKCSVTCGVGFQRRKQVCQKLTAKGRRVPLAETLCRDLPGLPLVRPCQMPVCSKLKLGMKTNLGERGPQ ILGVRRVYIQTREEKRINLTVGSRAYLLLNTSVIIKCPVRHFQKSLIQWEKDGHCLKNSKQLGITKSGSL KIHSLSTPDIGVYRCIAGSAQETVVLKLIGTDNRLIAHPSLREHARAYPGLDHNEANSLGATWHKMRQLW NSKNDLYLHGGEINKQPFLRALFGPCRTSEGNDNSWEFKSKQFEAAMKQGAYSMDTAQFDELIRNMSQLM ETGEASDDLASQMIYQLVAELAKAQPVQWRGTHEEVVASEAQFRGETGRVPKSPTVKNPGKLTFKPKGPV LMRQTLPPSVSFNKTINARIGNTVYITKSTEVINILCSLVTPSSEATYTWTKDGALLQPSVKIIVDETGK MQIQNPTRKEQGIYECSVASHQGSDVESSLVLYAEAPVILSIERNVTKPEHNHLSVVVGGIVEAALQANV TIHCPVKGVPQPNVTWLKRGGSLSDNVSLLFNGSLLLQNASLENEGTYICTATNALGKAVATSGLRLLER REPGNKTAFPKAQKKRVLQASKNRANSNNSTGKSLPQEYFWEPGNWTHCSATCGPLGVRLQRPQCMMASG QEVSEALCGPHRKPLAGFQSCNIRDCPARWFTSMWSECSVSCGGGSHSRQVMCKQTKANGTVQVVSPRAC ASKDRPLGRKPCSVRPCAQQATDLGSQCPGRCMGQAMRMQHHHTPCAHNSSNSDCEDRRRLAFRRNCTSG PCEACWRVGPWKPCTAVCGRGFQSRKVNCIHTGSCKPMADRYCVQMKPASWRHCFGPSCDRNCIDTTHYC MFVKHLNLCSLALYKQRCCQSCQER TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107533
ORF Size	5115 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107533.2, NP_001101003.2
RefSeq Size	7268 bp
RefSeq ORF	5118 bp
Locus ID	308787
MW	189.7 kDa

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