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Nudt5 (NM_001007733) Rat Tagged ORF Clone
CAT＃: RR211670
- TrueORF®
Nudt5 (Myc-DDK-tagged ORF) - Rat nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), (10 ug)
"NM_001007733" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC94209 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211670 representing NM_001007733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCAAGAACCAAAAGACTCTTCTCCCCCCACCGAGCAGCGCATTCTTTCAGAGGAGTTGATCT CAGAAGGAAAATGGGTCAAATTTGAAAAAACAACTTACATGGACCCCACCGGTAAAACTAGAACCTGGGA AACAGTGAAGCTTACGACCAGGAAGGGAAAATCTGCTGATGCCGTGTCAGTCATACCAGTCCTGCAAAGA ACGCTTCATCATGAGTGCATTGTCCTGGTGAAGCAGTTCCGGCCCCCGATGGGCGGCTACTGCCTGGAGT TCCCAGCAGGGCTCATCGAAGACGGGGAAAGCCCAGAAGCGGCTGCTCTGCGGGAGCTGGAGGAAGAGAC TGGCTACAAAGGTGACATTGCTGAATGCTCTCCAGCTGTGTGTATGGATCCAGGCTTGTCAAACTGCACC ACACACGTTGTGACAGTGACCATCAATGGAGATGACGCAGGAAATGTAAGGCCGAAACCCAAACCAGGGG ACGGAGAGTTTGTGGAAGTGATTTCTTTACCAAAGAATGATCTGCTGACGAGACTTGACGCTCTGGGTGC AGAAGACCGTCTTACAGTGGACGCCAGGGTCTACGCCTATGCTCTGGCGCTGAAACATGCCAACGCCAAG CCCTTCGAAGTGCCCTTCCTCAAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211670 representing NM_001007733
Red=Cloning site Green=Tags(s) METQEPKDSSPPTEQRILSEELISEGKWVKFEKTTYMDPTGKTRTWETVKLTTRKGKSADAVSVIPVLQR TLHHECIVLVKQFRPPMGGYCLEFPAGLIEDGESPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCT THVVTVTINGDDAGNVRPKPKPGDGEFVEVISLPKNDLLTRLDALGAEDRLTVDARVYAYALALKHANAK PFEVPFLKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007733 |
| ORF Size | 657 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007733.1, NP_001007734.1 |
| RefSeq Size | 1366 bp |
| RefSeq ORF | 660 bp |
| Locus ID | 361274 |
| UniProt ID | Q6AY63 |
| MW | 24.1 kDa |
| Gene Summary | Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211670 | Nudt5 (untagged ORF) - Rat nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), (10 ug) |
CNY 3,990.00 |
|
| RR211670L3 | Lenti ORF clone of Nudt5 (Myc-DDK-tagged ORF) - Rat nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), (10 ug) |
CNY 6,080.00 |
|
| RR211670L4 | Lenti ORF clone of Nudt5 (mGFP-tagged ORF) - Rat nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), (10 ug) |
CNY 6,650.00 |
