United States
Germany
Japan
United Kingdom
China
Trhde (NM_001108991) Rat Tagged ORF Clone
CAT＃: RR211716
- TrueORF®
Trhde (Myc-DDK-tagged ORF) - Rat thyrotropin-releasing hormone degrading enzyme (Trhde), (10 ug)
"NM_001108991" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 17,100.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAP-II |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211716 representing NM_001108991
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGACGGCGAGAGGGGGGAGCAAGAGGAGGAGAAGAAAAAGAAGAAGAAGAAAAAGAAGAGGA AGAAGAAGGAGGAGGAGGGGGCCGAGAAGAGCAGTTCACCGTTTGCGGCCACCATGGGAGAAGACGACGC CGCGCTCCGGGCAAGCGGCAGGGGGCTCTCGGACCCGTGGGCTGACTCGGTGGGAGTGAGACCCCGCACC ACGGAGCGCCACATCGCAGTGCACAAGAGGCTTGTGCTGGCTTTTGCCGTGTCCATCGTGGCACTGCTGG CTGTCACCATGCTGGCCGTACTGCTCAGCCTGCGGTTCGACGAGTGTGGAGCGAGCGCGGCGATGCCGGG CACCGACGGTGGCCTCGGAGGCTTCCCTGAGCGTGGTGGCAACAGCAGCTACCCAGGATCTGCCCGGCGC AACCACCACGCGGGTGAGGAATCCTCGCAGCGTGAGATCGGCGAGGTGGGCACCGCGGGGACCCCGTCTG CCCATCCGCCGTCGGAGGAAGAGCAGGAGCAGTGGCAGCCCTGGACTCAGCTGCGCCTATCCGGCCACCT TAAGCCGCTGCACTACAATTTGATGCTCACCGCCTTCATGGAGAACTTCACCTTCTCTGGGGAGGTCAAC GTGGAGATCGCGTGCCAGAACGCCACCCGCTACGTGGTACTGCACGCCTCCCGGGTGGCGGTGGAGAAGG TGCAAGTAGCGGAAGACCGGGCGTTCGGGGCTGTCCCGGTAGCAGGCTTTTTCCTCTACCCACAAACGCA GGTCTTGGTGGTGGTGCTGAATAGAACCCTGGATGCGCAGAGGCACTACAATCTGAAGATTATCTACAAT GCCCTGATAGAGAACGAGCTTTTGGGCTTCTTCCGCAGCTCCTACGTGATCCACGGGGAGAGAAGATTCC TCGGTGTTACTCAGTTTTCACCTACACATGCCAGGAAGGCATTTCCATGTTTTGATGAACCAATCTACAA GGCCACTTTCAAAATCAGCATCAAACATCAAGCAACCTATTTGTCTCTCTCCAACATGCCCGTGGAAACA TCTGTGTTTGAGGAAGATGGATGGGTAACAGATCACTTTTCACAGACCCCTCTCATGTCCACATATTATT TAGCCTGGGCGATTTGCAACTTCACATACAGAGAAACTACTACCAAGAGTGGGGTTGTAGTTCGATTATA TGCAAGACCCGACGCTATCAGAAGAGGATCCGGGGACTATGCTCTCCACATTACAAAGAGATTAATAGAA TTTTATGAAGACTACTTTAAAGTGCCCTATTCTTTGCCAAAACTAGATCTTTTAGCTGTGCCTAAGCATC CTTATGCTGCTATGGAGAACTGGGGACTAAGTATTTTTGTGGAACAAAGAATACTGCTGGATCCCAGTGT GTCATCTATTTCTTATTTGCTGGATGTCACCATGGTCATTGTTCATGAAATATGTCACCAGTGGTTTGGT GACCTTGTGACCCCAGTGTGGTGGGAAGATGTGTGGTTGAAGGAAGGCTTTGCTCACTACTTTGAATTTG TGGGTACAGACTACCTCTACCCTTCCTGGAACATGGAAAAGCAGAGGTTTCTGACAGATGTTCTGCATGA AGTGATGCTGCTTGACGGGTTGGCCAGTTCCCATCCAGTATCACAGGAAGTGCTACGGGCAACAGATATT GACAAAGTGTTTGACTGGATCGCATACAAAAAGGGTGCTGCTTTAATAAGAATGCTGGCTAATTTTATGG GCCATTCAGTTTTTCAGAGGGGATTGCAAGATTATTTAACCATTCACAAGTATGGCAATGCAGCCAGAAA TGATCTCTGGAATACATTATCAGAGGCTTTAAAAAGGAATGGAAAATATGTGAACATACAAGAGGTAATG GATCAGTGGACACTCCAGATGGGATATCCTGTTATCACCATCCTGGGGAACATGACGGCAGAAAACAGAA TACTCATCACCCAACAGCACTTCATTTACGATATCGGTGCTAAAACCAAAGCACTTCAACTTCAAAACAG CAGTTACCTGTGGCAGATTCCATTAACCATCGTCGTAGGAAATAGAAGCCATGTGTCTTCAGAAGCAATT ATTTGGGTGTCCAACAAATCAGAACATCACAGAATAACATACTTAGACAAAGGAAGCTGGATCCTTGGAA ACATCAATCAAACTGGCTACTTCAGAGTCAACTATGACCTAAGGAACTGGAGATTACTGATCGATCAGTT AATCAGGAACCATGAGGTACTTTCTGTCAGTAACCGTGCAGGCCTGATTGATGATGCCTTCAGCCTGGCC AGGGCAGGCTATTTGCCTCAGAATATTCCCCTAGAGATCATCAGATACCTCTCTGAGGAAAAGGATTTTC TTCCTTGGCATGCTGCCAGCCGAGCTCTTTATCCTCTGGATAAATTACTGGACCGCATGGAAAACTACAA TATCTTCAATGAATATATTCTAAAACAAGTGGCAACAACCTACAGCAAGCTTGGATGGCCAAAGAACAAC TTCAATGGCTCTGTCGTTCAAGCTTCCTATCAACATGAAGAGCTACGGAGGGAAGTGATAATGTTGGCCT GTAGCTTTGGGAACAAGCACTGTCACCAGCAGGCGTCAACTCTTATTTCAGACTGGATTTCCAGCAATAG AAACAGAATACCACTGAATGTTAGAGACATCGTCTACTGCACAGGAGTGTCCCTGCTGGATGAGGATGTC TGGGAATTCATCTGGATGAAATTCCACTCCACCACTGCAGTTTCTGAGAAAAAGATATTGCTGGAGGCGC TAACTTGCAGTGATGACAGGAATTTACTAAGTAGGCTTCTGAATCTGTCTCTGAATTCTGAGGTGGTGTT GGATCAAGATGCAATTGATGTAATAATCCACGTAGCCAGAAATCCACATGGCCGAGACCTTGCCTGGAAG TTTTTCAGAGATAAATGGAAAATACTAAATACCAGGTATGGAGAAGCATTATTTATGAATTCCAAACTTA TCAGTGGAGTTACAGAATTTCTTAATACAGAAGGTGAACTTAAAGAGCTAAAGAACTTCATGAAGTCCTA TGATGGGGTAGCATCTGCTTCTTTCTCACGAGCCGTGGAAACCGTGGAAGCCAATGTGCGCTGGAAAAGG CTTTATCAAGATGAGCTTTTCCAGTGGCTGGGAAAAGCCATGAGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211716 representing NM_001108991
Red=Cloning site Green=Tags(s) MALDGERGEQEEEKKKKKKKKKRKKKEEEGAEKSSSPFAATMGEDDAALRASGRGLSDPWADSVGVRPRT TERHIAVHKRLVLAFAVSIVALLAVTMLAVLLSLRFDECGASAAMPGTDGGLGGFPERGGNSSYPGSARR NHHAGEESSQREIGEVGTAGTPSAHPPSEEEQEQWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVN VEIACQNATRYVVLHASRVAVEKVQVAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRHYNLKIIYN ALIENELLGFFRSSYVIHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVET SVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIE FYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFG DLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPSWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLRATDI DKVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVM DQWTLQMGYPVITILGNMTAENRILITQQHFIYDIGAKTKALQLQNSSYLWQIPLTIVVGNRSHVSSEAI IWVSNKSEHHRITYLDKGSWILGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLA RAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYSKLGWPKNN FNGSVVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDV WEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLSRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWK FFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKSYDGVASASFSRAVETVEANVRWKR LYQDELFQWLGKAMRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108991 |
| ORF Size | 3198 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108991.1, NP_001102461.1 |
| RefSeq Size | 6678 bp |
| RefSeq ORF | 3201 bp |
| Locus ID | 366894 |
| MW | 122 kDa |
| Gene Summary | membrane-bound enzyme; catalyzes the hydrolysis of Thyrotropin-releasing hormone (TRH) at the pyroGlu-His bond [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211716 | Trhde (untagged ORF) - Rat thyrotropin-releasing hormone degrading enzyme (Trhde), (10 ug) |
CNY 17,100.00 |
|
| RR211716L3 | Lenti ORF clone of Trhde (Myc-DDK-tagged ORF) - Rat thyrotropin-releasing hormone degrading enzyme (Trhde), (10 ug) |
CNY 18,720.00 |
|
| RR211716L4 | Lenti ORF clone of Trhde (mGFP-tagged ORF) - Rat thyrotropin-releasing hormone degrading enzyme (Trhde), (10 ug) |
CNY 20,520.00 |
