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Spam1 (NM_053967) Rat Tagged ORF Clone
CAT＃: RR211737
- TrueORF®
Spam1 (Myc-DDK-tagged ORF) - Rat sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (Spam1), (10 ug)
"NM_053967" in other vectors (3)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Spam |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211737 representing NM_053967
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAGTTGCAATTTAAGTGGCTCTTTTGGAGGAGCTTTGCTGAGTCTGGGGGCACATTCCAAACAG TGTTAATCTTTCTTTTCATTCCATATTCCTTGACTGTGGATTATAGGGCAACACCAGTTCTGTCAGATAC AACTTTCGTTTGGGTCTGGAATGTTCCAACTGAAGCTTGTGTAGAAAACGTTACTGAACCAATAGATCTG AGCTTCTTCTCTTTAATTGGAAGCCCCCGGAAAACTGCCATAGGGCAGCCTGTCACATTATTTTATGTTG ATCGACTTGGTAACTATCCTCACATAGATGCACAGCAAACAGAACATCATGGAGGAATACCTCAGAAGGG GGATTTGACAACTCATTTGGTGAAAGCTAAGGAAGACGTAGAGCGTTACATACCAACAGACAAACTGGGT TTAGCTATCATTGACTGGGAAGAATGGAGGCCTACCTGGATGAGAAACTGGACACCTAAGGATATTTACA GGAATAAGTCTATTGAATTGGTCCAGGCAGCTGATCCAGCAATTAATATCACAGAAGCTACTGTGAGAGC CAAAGCACAATTTGAAGGGGCAGCTAAAGAGTTTATGGAAGGGACTTTAAAATTGGGGAAACATATTCGA CCAAAGCACTTATGGGGTTTTTATCTATTTCCTGATTGTTATAACAATAAATTTCAAGTCGATAACTATG ATGGCCAATGCCCTGATGTGGAAAAGAAACGAAACGATGATCTTGACTGGCTATGGAAAGAAAGCACGGG CCTTTACCCATCTGTCTATTTGAAGAAAGATTTGAAGTCGAGTCGAAAAGCAACCCTTTATGTCCGCTAC CGAGTTTTAGAATCTATCAGAGTGTCCAAAGTTTCAGATGAATCGAATCCAGTTCCAATTTTTGTCTATA TCCGTCTTGTTTTCACTGATCATGTCTCTGAATACCTTCTAGAGGATGATCTTGTGAATACAATTGGTGA AATTGTTGCTCAGGGTACCTCTGGAATTATAATATGGGATGCTATGTCTTTAGCACAACGTTCGGCAGGT TGCCCAATCCTACGTCAATACATGAAGACAACCCTGAATCCATACATAGTCAATGTCACCCTAGCAGCCA AAATGTGCAGTCAAACACTTTGTAAGGAGAAAGGCATGTGTTCGAGAAAAACAGAAAGTTCAGATGCATA TCTTCACTTGGATCCAAGTAGTTTTTCTATTAATGTAACAGAAGCTGGAAAGTATGAAGTTCTTGGCAAG CCCGAGGTTAAAGACTTGGAATACTTTTCTGAACATTTTAAATGCAGCTGTTTTAGCAAAATGACATGTG AGGAGACATCTGATATGCGAAGTATACAAGATGTGAATGTGTGCATGGGTGACAATGTTTGTATAAAAGC CACGCTAGGACCCAACTCAGCATTCCACCTCCTACCTGGCAAAGGCCTCCTCCTTATGACAACACTTGCT CACATCTTGCACCATCTACCACATGATATTTTTGTTTTTCCGTGGAAGATGCTAGTCAGTACTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211737 representing NM_053967
Red=Cloning site Green=Tags(s) MGELQFKWLFWRSFAESGGTFQTVLIFLFIPYSLTVDYRATPVLSDTTFVWVWNVPTEACVENVTEPIDL SFFSLIGSPRKTAIGQPVTLFYVDRLGNYPHIDAQQTEHHGGIPQKGDLTTHLVKAKEDVERYIPTDKLG LAIIDWEEWRPTWMRNWTPKDIYRNKSIELVQAADPAINITEATVRAKAQFEGAAKEFMEGTLKLGKHIR PKHLWGFYLFPDCYNNKFQVDNYDGQCPDVEKKRNDDLDWLWKESTGLYPSVYLKKDLKSSRKATLYVRY RVLESIRVSKVSDESNPVPIFVYIRLVFTDHVSEYLLEDDLVNTIGEIVAQGTSGIIIWDAMSLAQRSAG CPILRQYMKTTLNPYIVNVTLAAKMCSQTLCKEKGMCSRKTESSDAYLHLDPSSFSINVTEAGKYEVLGK PEVKDLEYFSEHFKCSCFSKMTCEETSDMRSIQDVNVCMGDNVCIKATLGPNSAFHLLPGKGLLLMTTLA HILHHLPHDIFVFPWKMLVSTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053967 |
| ORF Size | 1536 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053967.2, NP_446419.1 |
| RefSeq Size | 2112 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 117037 |
| UniProt ID | Q62803 |
| MW | 58.4 kDa |
| Gene Summary | a glycoprotein responsible for sperm adhesion to the zona pellucida [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211737 | Spam1 (untagged ORF) - Rat sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (Spam1), (10 ug) |
CNY 6,560.00 |
|
| RR211737L3 | Lenti ORF clone of Spam1 (Myc-DDK-tagged ORF) - Rat sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (Spam1), (10 ug) |
CNY 8,550.00 |
|
| RR211737L4 | Lenti ORF clone of Spam1 (mGFP-tagged ORF) - Rat sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (Spam1), (10 ug) |
CNY 9,220.00 |
