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Ddx4 (NM_001077647) Rat Tagged ORF Clone
CAT＃: RR211857
- TrueORF®
Ddx4 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), (10 ug)
"NM_001077647" in other vectors (3)
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CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RVLG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211857 representing NM_001077647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATGAAGATTGGGAGGCAGAAATCCTCAAACCTCATGTGTCTTCCTACGTTCCTGTATTTGAGA AGGATAAATATTCTTCTGGGGCAAATGGAGACACTTTTAACAGGACTTCAGCTTCATCATCAGAAATGGA AGATGGACCTTCTGGAAGAGATCATTTCATGAGAAGTGGATTTTCCTCTGGAAGAAATTTAGGAAACAGA GATATTGGCGAGTCTAGTAAAAGAGAGACTACATCTACAACCGGTGGCTTTGGAAGAGGAAAGGGTTTTG GAAACAGAGGTTTTTTAAATAACAAGTTTGAAGAAGGTGACAGCTCTGGTTTCTGGAAAGAGTCTACTAA TGACTGTGAAGATACTCAGACTCGGAGCAGAGGGTTTTCCAAGCGAGGCGGCTATCCAGATGGGAATGAT TCGGAAGCTTCAGGCCCATTCAGAAGAGGTGGGAGAGATAGTGAATATGACCAAGATCAGGGATCACAGC GTGGTGGTGGCCTTTTTGGTTCTAGGAAACCAGCAGCAAGTGATTCAGGCAGTGGTGACACTTTCCAGAG CAGAAGTGGGAATGCCCGAGGTGCCTACAAAGGCTTAAATGAAGAAGTAGTAACAGGCTCTGGAAAGAAT TCTTGGAAGTCAGAAGCTGAAGGAGGCGAAAGCAGTGATATTCAAGGTCCAAAAGTGACATATATACCCC CTCCTCCACCAGAGGATGAGGACTCCATCTTTGCACATTATCAGACAGGCATAAACTTTGATAAATATGA TACCATACTTGTTGAAGTATCTGGACATGATGCACCACCGGCAATTTTGACTTTTGAAGAAGCGAATCTC TGCCAGACCCTGAATAACAACATTGCTAAGGCTGGCTATACCAAGCTCACTCCTGTGCAGAAGTACAGCA TTCCCATTGTGTTAGCAGGAAGAGATTTGATGGCTTGTGCTCAAACAGGGTCTGGGAAGACGGCAGCTTT TCTCTTGCCTATTTTGGCTCATATGATGAGGGATGGAATAACTGCCAGTCGCTTTAAAGAACTGCAGGAA CCAGAGTGTATTATTGTAGCACCAACTCGAGAATTGATCAACCAAATTTATTTGGAAGCCAGAAAATTTT CTTTTGGGACTTGTGTAAGAGCTGTTGTCATATATGGAGGAACCCAGTTTGGGCACTCAATTCGACAGAT AGTGCAAGGGTGTAATATATTATGTGCTACTCCAGGGAGGCTGATGGATATCATAGGCAAAGAAAAGATT GGTCTCAAACAAGTCAAGTACTTAGTTTTGGATGAAGCTGATCGAATGTTGGATATGGGTTTTGGACCCG AAATGAAGAAGTTAATTTCTTGTCCAGGAATGCCATCAAAGGAACAGCGCCAAACTCTCTTATTCAGTGC AACTTTTCCAGAAGAAATCCAGAGGTTGGCTGGCGAGTTTTTAAAGTCAAATTATTTGTTTGTTGCTGTT GGACAAGTGGGAGGAGCTTGCAGAGATGTGCAGCAGTCCATTCTTCAAGTTGGCCCAGTATTCAAAAAGA GAAAACTTGTTGAGATTCTACGAAACATAGGTGATGAAAGACCTATGGTCTTTGTTGAAACCAAGAAAAA AGCAGATTTCATTGCGACTTTCCTTTGTCAAGAAAAAATATCAACTACAAGTATTCATGGCGATCGGGAA CAGAGGGAGCGAGAACAAGCTCTTGGAGATTTTCGCTGTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAG TGGCTGCCAGAGGACTTGATATTGAAAATGTTCAACATGTTATCAATTTTAACCTTCCTTCTACCATTGA TGAATATGTTCATCGAATTGGACGTACTGGACGTTGTGGAAATACTGGCAGGGCGATTTCTTTTTTTGAT ACCGAATCTGATAATCATTTAGCACAACCTCTAGTTAAAGTACTGTCAGATGCTCAACAGGATGTTCCTG CGTGGTTAGAAGAGATTGCCTTTAGTTCATATGCGCCTCCCAGCTTCAGTAATAGCACAAGAGGGGCTGT GTTTGCATCTTTTGACACTAGGAAGAATTTCCAGGGCAAGAACACACTGAACACAGCTGGGATTTCTTCT GCACAAGCTCCCAATCCAGTTGATGACGAGTCATGGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211857 representing NM_001077647
Red=Cloning site Green=Tags(s) MGDEDWEAEILKPHVSSYVPVFEKDKYSSGANGDTFNRTSASSSEMEDGPSGRDHFMRSGFSSGRNLGNR DIGESSKRETTSTTGGFGRGKGFGNRGFLNNKFEEGDSSGFWKESTNDCEDTQTRSRGFSKRGGYPDGND SEASGPFRRGGRDSEYDQDQGSQRGGGLFGSRKPAASDSGSGDTFQSRSGNARGAYKGLNEEVVTGSGKN SWKSEAEGGESSDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANL CQTLNNNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQE PECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSIRQIVQGCNILCATPGRLMDIIGKEKI GLKQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRLAGEFLKSNYLFVAV GQVGGACRDVQQSILQVGPVFKKRKLVEILRNIGDERPMVFVETKKKADFIATFLCQEKISTTSIHGDRE QREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFNLPSTIDEYVHRIGRTGRCGNTGRAISFFD TESDNHLAQPLVKVLSDAQQDVPAWLEEIAFSSYAPPSFSNSTRGAVFASFDTRKNFQGKNTLNTAGISS AQAPNPVDDESWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077647 |
| ORF Size | 2139 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077647.1, NP_001071115.1 |
| RefSeq Size | 3030 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 310090 |
| UniProt ID | Q64060 |
| MW | 78 kDa |
| Gene Summary | ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211857 | Ddx4 (untagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), (10 ug) |
CNY 9,120.00 |
|
| RR211857L3 | Lenti ORF clone of Ddx4 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), (10 ug) |
CNY 10,930.00 |
|
| RR211857L4 | Lenti ORF clone of Ddx4 (mGFP-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), (10 ug) |
CNY 12,070.00 |
