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Elf3 (NM_001024768) Rat Tagged ORF Clone
CAT＃: RR212170
- TrueORF®
Elf3 (Myc-DDK-tagged ORF) - Rat E74-like factor 3 (Elf3), (10 ug)
"NM_001024768" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212170 representing NM_001024768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCACCTGTGAGATCAGCAACGTTTTTAGCAACTACTTCAACGCCATGTACAGTTCAGAAGACC CCACCCTGGCTCCTGCTCCTCTGACTACTTTTGGCACTGAAGACTTTGTGTTGACCCTGAACAACCAACA CATGTCCCCAGAAGGTCCAGTAGGTTGTGTAGGACCTCAGACAAGATCCCAAAGGGACAGAACTGAGCCT CCGGCTGTACTCCACCTTGCAGAGAAGGCAAGCTGGACCGGAGAGCGGCCCCAGTTCTGGTCGAAGACCC AGGTACTGGATTGGATCAGCTACCAAGTGGAGAAGAACAAGTATGATGCCAGTTCCATTGACTTCTCGCG CTGCGACATGGACGGCGCCACCCTCTGCAACTGTGCGCTGGAGGAGCTGCGACTAGTCTTTGGCCCTCTG GGAGACCAACTCCATGCCCAGCTTCGGGACCTCACCTCCAGCTCTTCTGATGAACTTAGTTGGATCATCG AGCTGCTGGAGAAGGACGGCATGACCTTCCAAGAGGGCCTAGGAGACTCGGGTCCCTTTGACCAGGGAAG CCCTTTTGCCCAGGAGCTACTGGATGATGGCCGTCAGGCCAGCCCCTACTACGGCAGTTCCTATGGCCCT GGAGCGCCCTCTCCAGGTAGCTCTGATTTCTCCACTTCAGGGACCGATACTCCCCAGAGTTCCCACTCCT CTGACTCCGGTGGAAGTGATGTGGACCTGGACCTCACCGATAGCAAGGTCTTCCCTAGAGATGGCTTTCC TGACTATAAGAAGGGAGAACCCAAGCATGGAAAGAGGAAACGGGGGCGTCCCCGAAAGCTGAGCAAAGAA TACTGGGACTGTCTGGAGGGCAAGAAGAGCAAGCATGCCCCCAGAGGTACTCACCTGTGGGAGTTCATCC GGGACATTCTTATCCACCCTGAGCTCAACGAAGGCCTCATGAAATGGGAGAACCGGCACGAGGGCGTTTT CAAGTTTCTTCGTTCAGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAGAAGAATAGCAACATGACCTAT GAGAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAGATCCTGGAACGGGTGGATGGCCGTCGGC TCGTCTACAAGTTTGGCAAGAACTCCAGTGGCTGGAAGGAAGAAGAGGTTGGAGAGAGTCAGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212170 representing NM_001024768
Red=Cloning site Green=Tags(s) MAATCEISNVFSNYFNAMYSSEDPTLAPAPLTTFGTEDFVLTLNNQHMSPEGPVGCVGPQTRSQRDRTEP PAVLHLAEKASWTGERPQFWSKTQVLDWISYQVEKNKYDASSIDFSRCDMDGATLCNCALEELRLVFGPL GDQLHAQLRDLTSSSSDELSWIIELLEKDGMTFQEGLGDSGPFDQGSPFAQELLDDGRQASPYYGSSYGP GAPSPGSSDFSTSGTDTPQSSHSSDSGGSDVDLDLTDSKVFPRDGFPDYKKGEPKHGKRKRGRPRKLSKE YWDCLEGKKSKHAPRGTHLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTY EKLSRAMRYYYKREILERVDGRRLVYKFGKNSSGWKEEEVGESQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024768 |
| ORF Size | 1185 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024768.1, NP_001019939.1 |
| RefSeq Size | 1976 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 304815 |
| UniProt ID | Q4V7E1 |
| MW | 44.5 kDa |
| Gene Summary | Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212170 | Elf3 (untagged ORF) - Rat E74-like factor 3 (Elf3), (10 ug) |
CNY 3,990.00 |
|
| RR212170L3 | Lenti ORF clone of Elf3 (Myc-DDK-tagged ORF) - Rat E74-like factor 3 (Elf3), (10 ug) |
CNY 6,080.00 |
|
| RR212170L4 | Lenti ORF clone of Elf3 (mGFP-tagged ORF) - Rat E74-like factor 3 (Elf3), (10 ug) |
CNY 6,650.00 |
