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Hcn3 (NM_053685) Rat Tagged ORF Clone
CAT＃: RR212406
- TrueORF®
Hcn3 (Myc-DDK-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), (10 ug)
"NM_053685" in other vectors (3)
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CNY 10,070.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212406 representing NM_053685
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGAGGCGCGGCCGGCGGTGGGGGACGGGGAAGCGGCGACTCCTGCACGCGAGACGCCTCCTG CGGCTCCCGCCCAGGCCCGCGCGGCCTCAGGTGGGGTGCCAGAGTCTGCGCCCGAGCCGAAGAGGCGGCA GCTCGGGACGCTGCTGCAGCCGACCGTCAACAAGTTCTCTCTCCGGGTCTTCGGCAGCCACAAAGCGGTG GAAATCGAGCAGGAGAGGGTGAAGTCCGCCGGGGCCTGGATCATCCACCCCTACAGCGACTTCCGGTTTT ACTGGGACCTGATCATGCTGCTGCTGATGGTGGGGAACCTCATAGTACTGCCCGTGGGCATCACTTTCTT CAAGGAGGAGAACTCCCCACCCTGGATCGTCTTCAATGTCCTCTCGGACACTTTCTTCCTGCTGGATCTG GTGCTCAACTTCCGAACTGGCATCGTGGTGGAGGAAGGTGCGGAGATCCTGTTGGCGCCCAGGGCTATCC GCACGCGTTACCTGCGCACCTGGTTCCTGGTGGACCTGATTTCCTCCATCCCTGTGGATTACATCTTCCT AGTGGTAGAGCTGGAGCCACGACTAGACGCTGAGGTCTACAAAACGGCACGGGCCCTGCGCATCGTTAGA TTCACCAAGATCCTTAGCCTGCTGCGGCTGCTCCGCCTCTCCCGCCTCATCCGATACATGCACCAGTGGG AGGAGATCTTTCACATGACCTACGACCTGGCCAGTGCAGTGGTTCGCATCTTCAACCTCATTGGAATGAT GTTGCTGCTGTGTCACTGGGATGGCTGTCTGCAGTTCCTGGTCCCTATGCTGCAGGACTTCCCTTCCGAC TGCTGGGTCTCCATGAACCGCATGGTGAACCACTCGTGGGGCCGCCAGTACTCCCACGCCCTGTTCAAGG CCATGAGTCACATGCTGTGCATTGGCTACGGGCAGCAGGCACCAGTAGGCATGCCTGACGTCTGGCTCAC CATGCTCAGCATGATTGTGGGCGCCACCTGCTATGCCATGTTCATCGGCCACGCCACCGCCCTCATCCAG TCCCTGGACTCGTCCCGGCGCCAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCACA AGCTGCCAGCCGACACACGGCAGCGCATCCACGAGTACTACGAGCATCGGTACCAGGGCAAGATGTTCGA TGAAGAGAGCATCCTGGGGGAGCTGAGCGAGCCGCTTCGGGAGGAGATTATTAACTTCACCTGCCGGGGC CTGGTGGCCCACATGCCGCTGTTTGCTCATGCTGACCCCAGTTTCGTCACCGCAGTACTCACCAAGCTCC GTTTTGAGGTCTTCCAACCTGGGGATCTGGTGGTGCGTGAGGGCTCCGTGGGCAGGAAGATGTACTTCAT CCAGCATGGGCTGCTCAGTGTGTTGGCACGGGGCGCCCGGGACACTCGCCTCACTGACGGATCCTACTTT GGGGAGATCTGCCTGCTGACTCGAGGTCGGAGAACAGCCAGTGTAAGGGCTGACACCTACTGTCGCCTCT ACTCACTCAGCGTGGACCACTTCAATGCAGTGCTTGAGGAGTTCCCGATGATGCGCAGGGCTTTTGAGAC TGTGGCCATGGACCGGCTTCGGCGCATCGGCAAAAAGAATTCGATATTGCAGCGGAAACGCTCTGAGCCG AGTCCAGGCAGCAGCAGTGGTGGCGTCATGGAGCAGCATTTGGTACAACACGACAGAGACATGGCTCGTG GTATTCGGGGTCTGGCTCCGGGCACAGGAGCTCGCCTCAGTGGAAAGCCAGTGCTGTGGGAACCACTGGT ACACGCACCTCTGCAGGCAGCTGCTGTGACCTCCAACGTGGCCATAGCCTTGACTCATCAGCGAGGCCCT CTGCCCCTCTCCCCTGATTCTCCAGCCACCCTCCTGGCTCGATCTGCTAGACGCTCAGCAGGCTCCCCAG CCTCCCCACTGGTGCCTGTCCGAGCAGGTCCTCTGCTGGCCCGGGGACCCTGGGCGTCCACTTCTCGCCT GCCTGCTCCACCTGCCCGAACCCTCCATGCCAGCCTATCCCGGACAGGGCGTTCCCAGGTGTCTCTGTTG GGCCCTCCCCCAGGAGGAGGTGGTCGGAGGCTAGGACCTCGGGGCCGCCCACTCTCTGCCTCTCAACCCT CTCTGCCTCAGCGAGCCACGGGGGATGGCTCTCCTAGGCGCAAAGGCTCTGGAAGTGAGCGTCTGCCCCC CTCGGGGCTCCTGGCCAAGCCTCCAGGGACTGTCCAGCCATCCAGGTCATCAGTGCCTGAGCCAGTTACC CCCAGAGGTCCCCAAATTTCTGCCAACATG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR212406 representing NM_053685
Red=Cloning site Green=Tags(s) MEEEARPAVGDGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFSLRVFGSHKAV EIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDL VLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVR FTKILSLLRLLRLSRLIRYMHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSD CWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQ SLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRG LVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYF GEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEP SPGSSSGGVMEQHLVQHDRDMARGIRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGP LPLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLL GPPPGGGGRRLGPRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPSRSSVPEPVT PRGPQISANM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053685 |
| ORF Size | 2340 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053685.1, NP_446137.1 |
| RefSeq Size | 2343 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 114245 |
| UniProt ID | Q9JKA8 |
| MW | 86.8 kDa |
| Gene Summary | may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212406 | Hcn3 (untagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), (10 ug) |
CNY 10,070.00 |
|
| RR212406L3 | Lenti ORF clone of Hcn3 (Myc-DDK-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), (10 ug) |
CNY 11,880.00 |
|
| RR212406L4 | Lenti ORF clone of Hcn3 (mGFP-tagged ORF) - Rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), (10 ug) |
CNY 13,020.00 |
