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Krt25 (NM_001008822) Rat Tagged ORF Clone
CAT＃: RR212503
- TrueORF®
Krt25 (Myc-DDK-tagged ORF) - Rat keratin 25 (Krt25), (10 ug)
"NM_001008822" in other vectors (3)
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CNY 5,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ka38; Krt25A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212503 representing NM_001008822
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTTCGCCTCTCCAGTGGATCCAAGAGGTCCTATGCTCGTCCCAGCACAGGATCGCTCAGGGGCG CCAGCTTTGGTGCTGCAAATGCTTGTGGCGTAGCAGGCATTGGAAGTGGCTTCTCCTGCGCCTTTGGGAG CAGCTCCACAGGAGGAAACACGGGTGTGGCCAACTCCTGTGCTGGCTTCACTGTGAATGAGGGGGGCCTC CTCTCCGGCAATGAGAAGGTGACCATGCAGAACCTCAATGACCGCCTGGCCTCCTACCTGGATAATGTAC AAGCACTTCAGGAGGCCAACGCTGACCTGGAGCAGAAGATCAAGGGCTGGTATGAAAAATTTGGACCTGG GTCATGCCGCGGTCTCGATCATGACTACAGTAGATACTTCCCCATCATTGATGATCTTAAAAACCAGATC ATCACTTCCACCACCAGCAATGCCAATGCCGTTCTGCAGATTGACAATGCCAGGCTCACGGCTGATGACT TCAGACTCAAGTACGAAAACGAGCTGGCCCTCCACCAGAGCGTAGAGGCTGACGTGAACGGGCTGCGCAG GGTTCTGGATGAAATCACCCTGTGCCGCACTGACCTGGAGATTCAGTACGAAACCCTGAGTGAGGAACTG ACTTACCTCAAGAAGAACCACAAAGAGGAAATGCAGGCTCTGCAGTGCGCAGCCGGAGGTAATGTGAATG TGGAGATGAACGCAGCCCCCGGCGTGGACCTCACCGTCCTGCTGAACAACATGCGAGCCGAGTACGAGGC CCTGGCTGAGCAGAACCGCAGGGACGCCGAGGCCTGGTTCCAAGAGAAGAGCGCTTCGCTGCAGCAGCAG ATCACCGAGGACGTGGGCGCCACCACCTCAGCCAGGAATGAGCTGACGGAAATGAAACGTACGCTCCAAA CCTTGGAGATTGAACTACAGTCTCTCCTGGCCACGAAACACTCTCTGGAGTGCTCCCTGACGGAGACCGA GGGCAACTACTGCACGCAGCTGGCTCAGATCCAGGCTCAGATCAGCGCCCTGGAGGAGCAGCTGCACCAG GTGAGGACGGAGACCGAGGGCCAGAAGCTGGAATATGAGCAGCTGCTGAACGTCAAGGCCCACCTGGAGA AGGAGATCGAGACCTACTGCCTCCTCATAGGAGGAGACGAGGGGGCCTGTAAGTCTTCGAGTTACAAGTC TAAAGATTACACCTCTGGAAACGCGGGAAACCAAAGCAAAGATTCCCCCAAAGCCATAGTGGTTAAGAAA GTCCTAGAGGAGGTGGACCAACGCAGCAAGATTCTAACCACTAGGCTCCACTCCCTGGAAGAGAAATCTC AAAGCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212503 representing NM_001008822
Red=Cloning site Green=Tags(s) MSLRLSSGSKRSYARPSTGSLRGASFGAANACGVAGIGSGFSCAFGSSSTGGNTGVANSCAGFTVNEGGL LSGNEKVTMQNLNDRLASYLDNVQALQEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDDLKNQI ITSTTSNANAVLQIDNARLTADDFRLKYENELALHQSVEADVNGLRRVLDEITLCRTDLEIQYETLSEEL TYLKKNHKEEMQALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQEKSASLQQQ ITEDVGATTSARNELTEMKRTLQTLEIELQSLLATKHSLECSLTETEGNYCTQLAQIQAQISALEEQLHQ VRTETEGQKLEYEQLLNVKAHLEKEIETYCLLIGGDEGACKSSSYKSKDYTSGNAGNQSKDSPKAIVVKK VLEEVDQRSKILTTRLHSLEEKSQSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008822 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008822.1, NP_001008822.1 |
| RefSeq Size | 1341 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 303519 |
| UniProt ID | Q6IFX0 |
| MW | 48.9 kDa |
| Gene Summary | Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs) (By similarity). Plays a role in the cytoskeleton organization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212503 | Krt25 (untagged ORF) - Rat keratin 25 (Krt25), (10 ug) |
CNY 5,700.00 |
|
| RR212503L3 | Lenti ORF clone of Krt25 (Myc-DDK-tagged ORF) - Rat keratin 25 (Krt25), (10 ug) |
CNY 7,600.00 |
|
| RR212503L4 | Lenti ORF clone of Krt25 (mGFP-tagged ORF) - Rat keratin 25 (Krt25), (10 ug) |
CNY 8,460.00 |
