Prkn (NM_020093) Rat Tagged ORF Clone
CAT#: RR212553
- TrueORF®
Park2 (Myc-DDK-tagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug)
"NM_020093" in other vectors (3)
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CNY 3,656.00
CNY 6,080.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Park; Park2; Prkn |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212553 representing NM_020093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGTGTTTGTCAGGTTCAACTCCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCT TCCAGCTCAAGGAAGTGGTTGCTAAGCGACAGGGGGTTCCAGCTGACCAGCTGCGAGTGATTTTTGCTGG GAAGGAGCTGCAGAATCACCTGACAGTACAGAACTGTGACCTGGAACAACAGAGTATCGTTCACATAGTA CAGAGACCACAGAGGAAAAGTCACGAAACAAATGCCTCTGGAGGGGACAAACCCCAGAGCACCCCGGAGG GCTCCATATGGGAGCCCAGAAGCTTGACTCGAGTGGACCTCAGCAGCCATATCCTGCCAGCGGACTCCGT GGGGCTGGCAGTCATTCTGGACACAGACAGCAAGAGTGACTCAGAAGCAGCCAGAGGTCCAGCAGCTAAA CCCACCTACCACAGCTTTTTTGTCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTGGGAAACTCCGAG TTCAGTGCGGCACCTGCAGACAAGCAACCCTCACCTTGGCCCAGGGCCCATCTTGCTGGGATGATGTCTT AATTCCAAACCGGATGAGTGGAGAGTGCCAATCTCCAGACTGCCCTGGGACAAGAGCTGAATTTTTCTTT AAATGTGGAGCACACCCAACCTCAGACAAGGACACATCAGTAGCTTTGAACCTGATCACCAACAACAGCC GCAGCATCCCCTGCATCGCGTGCACGGATGTCAGGAACCCTGTCTTGGTCTTCCAATGTAACCACCGCCA CGTGATCTGTTTGGACTGCTTCCACTTGTACTGTGTCACAAGGCTCAACGATCGGCAGTTTGTCCACGAC GCTCAACTTGGCTACTCCCTGCCGTGTGTGGCTGGCTGTCCCAACTCCCTGATTAAAGAGCTCCATCACT TCAGGATCCTTGGAGAAGAGCAGTACAACAGGTACCAGCAGTATGGTGCCGAGGAGTGCGTGCTGCAGAT GGGAGGTGTGCTGTGCCCCCGTCCTGGCTGCGGAGCTGGGCTGCTGCCTGAACAGGGCCAGAGGAAAGTC ACCTGCGAAGGGGGCAACGGCCTGGGCTGTGGGTTCGTTTTCTGCCGGGACTGCAAGGAAGCATACCATG AAGGGGAGTGCGACTCGATGTTCGAAGCCTCGGGGGCGACTTCTCAGGCATACCGGGTGGATCAAAGAGC TGCTGAGCAAGCACGGTGGGAGGAGGCCTCCAAGGAAACCATCAAGAAAACCACCAAGCCTTGTCCTCGC TGCAATGTGCCCATTGAAAAGAATGGAGGATGTATGCACATGAAGTGTCCTCAGCCCCAGTGCAAGCTGG AGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTGAGTGG TGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212553 representing NM_020093
Red=Cloning site Green=Tags(s) MIVFVRFNSSYGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELQNHLTVQNCDLEQQSIVHIV QRPQRKSHETNASGGDKPQSTPEGSIWEPRSLTRVDLSSHILPADSVGLAVILDTDSKSDSEAARGPAAK PTYHSFFVYCKGPCHKVQPGKLRVQCGTCRQATLTLAQGPSCWDDVLIPNRMSGECQSPDCPGTRAEFFF KCGAHPTSDKDTSVALNLITNNSRSIPCIACTDVRNPVLVFQCNHRHVICLDCFHLYCVTRLNDRQFVHD AQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKV TCEGGNGLGCGFVFCRDCKEAYHEGECDSMFEASGATSQAYRVDQRAAEQARWEEASKETIKKTTKPCPR CNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWNRACMGDHWFDVSGAGTVAVSGTEPAWVITGLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020093 |
ORF Size | 1395 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1564 bp |
RefSeq ORF | 1398 bp |
Locus ID | 56816 |
UniProt ID | Q9JK66 |
MW | 51.7 kDa |
Gene Summary | localized to the cytoplasm in neurons of many regions of the brain; has N-terminal ubiquitin and ring-IBR-ring finger domains [RGD, Feb 2006] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Trib3 Is Elevated in Parkinson's Disease and Mediates Death in Parkinson's Disease Models
,Aimé, P;Sun, X;Zareen, N;Rao, A;Berman, Z;Volpicelli-Daley, L;Bernd, P;Crary, JF;Levy, OA;Greene, LA;,
J. Neurosci.
,PubMed ID 26224857
[Prkn]
|
ATF4 Protects Against Neuronal Death in Cellular Parkinson's Disease Models by Maintaining Levels of Parkin
,Xiaotian Sun, Jin Liu, John F. Crary, Cristina Malagelada, David Sulzer, Lloyd A. Greene, and Oren A. Levy,
J. Neurosci., Feb 2013; 33: 2398 - 2407.
[Prkn]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212553 | Park2 (untagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug) |
CNY 6,080.00 |
|
RR212553L3 | Lenti ORF clone of Park2 (Myc-DDK-tagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug) |
CNY 7,890.00 |
|
RR212553L4 | Lenti ORF clone of Park2 (mGFP-tagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug) |
CNY 8,650.00 |