Abcc2 (NM_012833) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cmoat; cMRP; mrp; Mrp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212798 representing NM_012833 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGTTCTGCAACTCTACTTTTTGGGATCTCTCATTACTGGAAAGTCCAGAGGCTGACCTGCCTC TTTGTTTTGAGCAAACTGTTCTGGTGTGGATTCCCTTGGGCTTTCTTTGGCTCCTGGCTCCTTGGCAACT TTACAGCGTGTACAGATCCAGGACCAAGAGATCTTCTATAACCAAATTCTACCTTGCCAAGCAGGTGTTC GTCGTGTTTCTTCTTATTTTAGCAGCCATAGACCTGTCTCTTGCGCTCACAGAAGATACTGGACAAGCCA CAGTTCCTCCTGTCAGATATACGAATCCAATCCTCTACCTGTGCACATGGCTCCTGGTTTTGGCAGTCCA GCACAGCAGGCAATGGTGTGTACGAAAGAACTCTTGGTTCCTGTCTCTGTTCTGGATCCTCTCGGTCTTA TGCGGCGTATTCCAGTTTCAGACTCTGATACGAGCACTCCTGAAGGACAGCAAGTCCAACATGGCCTACT CCTACCTGTTCTTCGTCTCCTACGGTTTCCAGATTGTCCTCCTGATTCTTACAGCCTTTTCAGGACCAAG TGACTCAACACAAACTCCATCAGTCACGGCTTCCTTTCTGAGTAGCATTACATTTAGTTGGTATGACAGG ACTGTTCTGAAAGGTTACAAGCATCCACTGACACTAGAAGATGTCTGGGATATCGATGAAGGGTTTAAAA CAAGGTCAGTCACCAGCAAGTTTGAGGCGGCCATGACAAAGGACCTGCAGAAAGCCAGGCAGGCTTTTCA GAGGCGGCTGCAGAAGTCCCAGCGGAAACCTGAGGCCACACTACACGGACTGAACAAGAAGCAGAGTCAG AGCCAAGACGTTCTCGTCCTGGAAGAAGCGAAAAAGAAGTCTGAGAAGACCACCAAAGACTATCCCAAAT CGTGGTTGATCAAGTCTCTCTTCAAAACCTTCCACGTAGTGATCCTGAAATCATTTATACTGAAATTAAT ACATGACCTTTTGGTGTTTCTGAATCCTCAGCTGCTGAAGTTGCTGATCGGTTTCGTGAAGAGCTCTAAC TCATACGTGTGGTTTGGCTATATCTGTGCAATCCTAATGTTTGCTGTGACTCTCATCCAATCTTTCTGCC TTCAGTCTTACTTTCAACATTGTTTTGTGTTGGGAATGTGCGTACGGACAACCGTCATGTCTTCGATATA TAAGAAGGCATTGACCCTATCTAACTTGGCTAGGAAGCAGTACACCATTGGAGAGACGGTGAACTTGATG TCTGTAGATTCCCAGAAGCTAATGGATGCGACCAACTACATGCAGTTGGTGTGGTCAAGTGTTATACAGA TTACTTTGTCCATCTTCTTCCTGTGGAGAGAGTTGGGACCGTCCATCTTAGCAGGTGTTGGGGTTATGGT TCTCCTAATCCCAGTTAATGGAGTTCTGGCTACCAAGATCAGAAATATTCAGGTCCAAAATATGAAGAAT AAAGACAAACGTTTAAAAATCATGAATGAGATTCTCAGTGGAATCAAGATCCTGAAATACTTTGCCTGGG AACCTTCATTTCAAGAGCAAGTCCAGGGCATTCGGAAGAAAGAACTCAAGAACTTGCTGCGGTTCGGCCA GCTGCAGAGTCTGCTGATCTTCATTTTACAGATAACTCCAATCCTGGTGTCTGTGGTCACATTTTCTGTC TATGTCCTGGTGGATAGCGCCAATGTTTTGAATGCGGAGAAGGCATTTACCTCCATCACCCTCTTCAATA TCCTACGCTTCCCTCTGTCCATGCTTCCCATGGTGACCTCATCGATCCTCCAGGCCAGTGTTTCTGTGGA CCGGCTGGAGAGGTATTTGGGAGGAGACGATTTAGACACATCTGCCATTCGCCGCGTCAGCAATTTTGAT AAAGCTGTGAAGTTTTCAGAGGCCTCTTTTACTTGGGACCCGGACTTGGAAGCCACAATCCAAGATGTGA ACCTGGACATAAAGCCAGGCCAACTGGTGGCTGTGGTGGGCACTGTAGGCTCTGGGAAATCCTCTTTGGT ATCAGCCATGCTGGGAGAAATGGAAAACGTTCACGGGCACATCACCATCCAGGGATCCACAGCCTATGTC CCTCAGCAGTCCTGGATTCAGAATGGAACCATCAAAGACAACATCCTGTTTGGGTCCGAATACAATGAAA AGAAGTACCAGCAAGTTCTCAAAGCATGCGCTCTCCTCCCAGACTTGGAAATATTGCCTGGAGGAGACAT GGCTGAGATCGGAGAGAAGGGGATAAATCTCAGTGGTGGTCAGAAGCAGCGAGTCAGCCTGGCCAGAGCT GCCTATCAAGATGCTGACATCTATATTCTGGACGATCCCCTGTCGGCTGTGGATGCTCATGTGGGAAAAC ACATTTTCAACAAGGTTGTGGGCCCCAACGGCCTGTTGGCTGGCAAGACGAGAATCTTTGTTACTCATGG TATTCACTTCCTTCCCCAAGTGGATGAGATTGTAGTTCTGGGGAAAGGCACCATCTTAGAGAAAGGATCC TATCGTGACCTGTTGGACAAGAAGGGAGTGTTTGCTAGGAACTGGAAGACCTTCATGAAGCATTCAGGGC CTGAAGGAGAGGCCACAGTCAATAATGACAGTGAGGCGGAAGACGACGATGATGGGCTGATTCCCACCAT GGAGGAAATCCCTGAGGATGCAGCTTCCTTGGCCATGAGAAGAGAAAATAGTCTTCGCCGTACACTGAGC CGCAGCTCTAGGTCCAGCAGCCGACGTGGGAAGTCCCTCAAAAACTCCTTGAAGATTAAAAATGTGAATG TCTTGAAGGAGAAGGAAAAAGAAGTGGAAGGACAAAAACTAATTAAGAAAGAATTTGTGGAAACCGGGAA GGTCAAGTTCTCCATCTACCTGAAGTATCTACAGGCAGTAGGGTGGTGGTCCATACTTTTCATCATCCTT TTCTACGGATTGAATAATGTTGCTTTTATCGGCTCTAACCTCTGGCTGAGTGCTTGGACCAGTGACTCTG ACAACTTGAATGGGACCAACAATTCGTCTTCTCATAGGGACATGAGAATTGGGGTCTTTGGAGCTCTGGG ATTAGCACAAGGTATATGTTTGCTTATTTCAACTCTGTGGAGCATATATGCTTGCAGAAATGCATCAAAA GCTTTGCACGGGCAGCTGTTAACCAACATCCTCCGGGCACCCATGAGGTTTTTTGACACAACTCCCACAG GCCGGATTGTGAACAGATTTTCTGGTGATATTTCTACTGTGGACGACTTGCTCCCCCAGACACTTCGAAG CTGGATGATGTGTTTCTTTGGCATCGCTGGCACTCTTGTCATGATCTGCATGGCCACCCCAGTCTTCGCT ATCATCATCATTCCTCTCAGCATTCTTTATATTTCGGTGCAGGTTTTTTATGTGGCTACTTCCCGCCAGC TGAGACGGTTGGATTCTGTCACCAAATCTCCGATCTATTCTCACTTCAGTGAGACTGTCACAGGTTTGCC CATTATCCGTGCCTTTGAGCACCAGCAGCGATTTCTAGCTTGGAATGAGAAGCAGATTGACATCAACCAG AAATGTGTCTTTTCCTGGATTACCTCCAACAGGTGGCTTGCAATTCGGCTGGAGCTGGTTGGAAACTTGG TCGTCTTCTGTTCCGCCTTGCTGCTGGTTATTTATAGAAAAACCTTAACCGGGGACGTTGTGGGCTTTGT TCTGTCCAACGCCCTCAATATCACACAAACCTTGAACTGGCTAGTGAGGATGACGTCAGAAGCAGAGACC AACATTGTGGCAGTTGAGCGAATAAGTGAATACATAAATGTAGAGAATGAGGCGCCCTGGGTGACTGACA AGAGGCCTCCGGCAGACTGGCCCAGACATGGTGAGATCCAGTTTAACAACTATCAAGTGCGGTATCGGCC GGAGCTGGATCTGGTACTGAAAGGGATCACTTGTAACATCAAGAGCGGAGAGAAGGTCGGCGTAGTGGGC AGGACTGGGGCTGGGAAATCATCCCTCACAAACTGCCTCTTCAGAATCTTAGAGTCTGCGGGGGGCCAGA TCATCATTGATGGGATAGATGTTGCCTCCATTGGACTGCACGACCTTCGAGAGAGGCTGACCATCATTCC CCAGGACCCCATTTTGTTCTCGGGGAGTCTGAGGATGAATCTCGACCCTTTCAACAAATATTCAGATGAG GAGGTTTGGAGGGCCCTGGAGTTGGCTCACCTCAGATCCTTTGTGTCTGGCCTACAGCTTGGGTTGTTAT CCGAAGTGACAGAGGGTGGTGACAACCTGAGCATAGGGCAGAGGCAGCTCCTATGCCTGGGCAGGGCTGT GCTTCGAAAATCCAAAATCCTGGTCCTGGATGAAGCCACGGCTGCAGTGGATCTCGAGACGGATAGCCTC ATTCAGACGACCATCCGAAAGGAGTTCTCCCAGTGCACGGTCATCACCATCGCTCACAGGCTGCACACCA TCATGGACAGTGACAAGATAATGGTCCTAGACAACGGGAAGATTGTCGAGTATGGCAGTCCTGAAGAACT GCTGTCCAACAGAGGTTCCTTCTATCTGATGGCCAAGGAAGCCGGCATTGAAAATGTGAATCACACAGAG CTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212798 representing NM_012833 Red=Cloning site Green=Tags(s) MDKFCNSTFWDLSLLESPEADLPLCFEQTVLVWIPLGFLWLLAPWQLYSVYRSRTKRSSITKFYLAKQVF VVFLLILAAIDLSLALTEDTGQATVPPVRYTNPILYLCTWLLVLAVQHSRQWCVRKNSWFLSLFWILSVL CGVFQFQTLIRALLKDSKSNMAYSYLFFVSYGFQIVLLILTAFSGPSDSTQTPSVTASFLSSITFSWYDR TVLKGYKHPLTLEDVWDIDEGFKTRSVTSKFEAAMTKDLQKARQAFQRRLQKSQRKPEATLHGLNKKQSQ SQDVLVLEEAKKKSEKTTKDYPKSWLIKSLFKTFHVVILKSFILKLIHDLLVFLNPQLLKLLIGFVKSSN SYVWFGYICAILMFAVTLIQSFCLQSYFQHCFVLGMCVRTTVMSSIYKKALTLSNLARKQYTIGETVNLM SVDSQKLMDATNYMQLVWSSVIQITLSIFFLWRELGPSILAGVGVMVLLIPVNGVLATKIRNIQVQNMKN KDKRLKIMNEILSGIKILKYFAWEPSFQEQVQGIRKKELKNLLRFGQLQSLLIFILQITPILVSVVTFSV YVLVDSANVLNAEKAFTSITLFNILRFPLSMLPMVTSSILQASVSVDRLERYLGGDDLDTSAIRRVSNFD KAVKFSEASFTWDPDLEATIQDVNLDIKPGQLVAVVGTVGSGKSSLVSAMLGEMENVHGHITIQGSTAYV PQQSWIQNGTIKDNILFGSEYNEKKYQQVLKACALLPDLEILPGGDMAEIGEKGINLSGGQKQRVSLARA AYQDADIYILDDPLSAVDAHVGKHIFNKVVGPNGLLAGKTRIFVTHGIHFLPQVDEIVVLGKGTILEKGS YRDLLDKKGVFARNWKTFMKHSGPEGEATVNNDSEAEDDDDGLIPTMEEIPEDAASLAMRRENSLRRTLS RSSRSSSRRGKSLKNSLKIKNVNVLKEKEKEVEGQKLIKKEFVETGKVKFSIYLKYLQAVGWWSILFIIL FYGLNNVAFIGSNLWLSAWTSDSDNLNGTNNSSSHRDMRIGVFGALGLAQGICLLISTLWSIYACRNASK ALHGQLLTNILRAPMRFFDTTPTGRIVNRFSGDISTVDDLLPQTLRSWMMCFFGIAGTLVMICMATPVFA IIIIPLSILYISVQVFYVATSRQLRRLDSVTKSPIYSHFSETVTGLPIIRAFEHQQRFLAWNEKQIDINQ KCVFSWITSNRWLAIRLELVGNLVVFCSALLLVIYRKTLTGDVVGFVLSNALNITQTLNWLVRMTSEAET NIVAVERISEYINVENEAPWVTDKRPPADWPRHGEIQFNNYQVRYRPELDLVLKGITCNIKSGEKVGVVG RTGAGKSSLTNCLFRILESAGGQIIIDGIDVASIGLHDLRERLTIIPQDPILFSGSLRMNLDPFNKYSDE EVWRALELAHLRSFVSGLQLGLLSEVTEGGDNLSIGQRQLLCLGRAVLRKSKILVLDEATAAVDLETDSL IQTTIRKEFSQCTVITIAHRLHTIMDSDKIMVLDNGKIVEYGSPEELLSNRGSFYLMAKEAGIENVNHTE L TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012833
ORF Size	4623 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012833.2, NP_036965.1
RefSeq Size	5073 bp
RefSeq ORF	4626 bp
Locus ID	25303
UniProt ID	Q63120
MW	173.4 kDa
Gene Summary	multispecific organic anion transporter; mutation may be responsible for conjugated hyperbilirubinemia in the TR- rat, which is a model for human Dubin-Johnson syndrome [RGD, Feb 2006]

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