Nup153 (NM_001100470) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NUCZINK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213083 representing NM_001100470 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGGAGCCGGAGGCATAGGAGGGGGCGGCGGCGGCGGCAAGATCCGGACGCGTCGTTGCCACC AGGGGCCGGTGAAGCCTTACCAGCAGGGGCGACCCCAACACCAGGGCATTCTTAGCAGGGTTACAGAATC TGTTAAGAATATTGTACCAGGGTGGCTACAGAGATACTTCAACAAGAGTGAAAATGCCTGTAGCTGTTCA GTAAATGCAGATGAAGTTCCACGTTGGCCGGAAAATAGAGAAGATGAGCGTGAGATTTATGTGGATGAGA ACACAAACACTGATGACGGGAGAACCACCCCTGAGCCCACAGGCAGTAACACAGAAGAACCATCAACCAC CAGTACTGCTTCAAATTATCCAGATGTATTAACAAGACCTTCTCTTCATCGGAGTCATCTGAATTTCTCC GTGTTAGAATCCCCTGCTTTGCACTGTCAGCCATCCACGTCCTCTGCATTCCCAATCGGCAGTTCTGGAT TTTCCCTTGTGAAGGAGATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGG GTTCTCTTCAAGAGCTTCTGAGAAAGATATAGCTGTTTCAAAGAACACTTCCCTGCCACCTTTGTGGTCC CCCGAAGCAGAGCGTTCTCATTCACTCTCACAACATACTGCCATCAGCTCCAAAAAACCAGCATTCAACT TGTCTGCCTTTGGAACACTTTCCACCTCACTTGGGAATTCTTCAATTCTTAAAACAAGTCAACTTGGAGA CTCTCCTTTTTATCCTGGCAAAACAACATATGGTGGGGCAGCTGCTGCTGTAAGACAGAATAAAGTACGA AGTACACCTTACCAGGCTCCAGTTCGAAGACAGATGAAAGCCAAGCAGCTCAATGCACAGTCTTATGGTG TGACCAGTTCAACAGCACGGCGGATATTGCAGTCATTAGAAAAGATGTCAAGCCCTCTTGCGGATGCAAA AAGAATTCCATCTGCTGTTTCTTCTCCTCTGAATTCTCCCCTTGATAGGAGTGGGATAGATAGCACAGTT TTTCAGGCAAAAAAGGAAAAGGTGGACTCTCAGTATCCCCCTGTTCAGAGACTAATGACCCCAAAGCCAG TTTCCATAGCAACAAATAGAACTGTCTATTTTAAACCATCTCTGACCCCATCTGGTGACTTAAGGAAGAC TAATCAAAGGATAGATAAAAAGAACAGTACTGTAGATGAAAAAAATATCTCAAGACAAAATAGAGAGCAA GAAAGTGGCTTTTCATACCCCAATTTCAGTATTCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTGGTG GAGGTGGCAAGATGAGAAGAGAGAGAACAACACATTTTGTTGCTTCTAAACCTTCAGAGGAGGAGGAAGT AGAAGTACCATTGTTACCGCAAATCTCTCTACCAATCAGCAGTTCTTCACTGCCCACCTTCAACTTTAGT TCCCCTGCGATCTCGGCTGCTTCTTCGTCATCAGTCAGCCCTTCTCAGCCATTATCAAACAAGGTACAGA TGACCTCTCTGGGCAGCACAGGCAATCCCGTGTTCACCTTTTCCTCTCCCATTGTGAAGTCCACGCAGGC AGACGTGCTACCTCCAGCATCTATTGGATTTACGTTTAGTGTGCCTCTTGCAAAAACAGAACTTTCTGGC CCTAATAGTTCTTCAGAAACAGTTTTAAGTAGTTCAGTTACAGCTCAAGATAACACTGTAGTGAATAGTT CAAGCTCTAAGAAGCGAAGTGCCCCTTGTGAGGACCCTTTTACACCTGCAAAGATCCTGAGAGAAGGAAG TGTTTTAGACATTCTGAAAACGCCTGGTTTCATGTCACCAAAGGTTGATTCTCCTGCTCTTCAGCCCACA ACTACAAGCTCAATAGTTTATACAAGACCAGCAATAAGTACTTTTTCTTCTAGTGGGGTTGAGTTTGGAG AAAGTTTAAAAGCTGGATCATCATGGCAGTGTGACACATGTCTACTCCAGAACAAAGTCACAGACAATAA GTGTATAGCCTGTCAAGCAGCAAAACTGCCACTGAAAGAAACTGCTAAACAGACTGGAATTGGGACACCA AGCAAAAGTGACAAGCCAGCTTCTACATCAGGGACAGGTTTTGGAGATAAATTTAAACCAGCAATAGGAA CTTGGGATTGTGATACCTGTTTAGTGCAAAATAAGCCTGAAGCAGTAAAATGTGTAGCCTGTGAAACACC CAAACCTGGCACTGGTGTGAAGCGAGCCCTTCCATTGACGGTGGCTTCAGAAAGTCCTGTGACTGCTTCC TCTTCTACCACCGTGACCACTGGTACACTAGGATTTGGAGATAAATTCAAAAGGCCCGTGGGATCTTGGG AGTGTCCGGTATGCTGTGTTTCTAATAAGGCAGAAGACAGCAGATGTGTCTCCTGTACATCTGAGAAACC AGGTTTGGTATCTGCCTCAAGCAGCAACCCTGTCCCTGTCTCTCTGCCTTCTGGAGGCTGTTTAGGATTG GACAAGTTTAAAAAACCAGAGGGAAGCTGGGACTGTGAAGTATGCCTAGTGCAAAATAAAGCGGACTCTA CCAAGTGCATAGCTTGTGAAAGTGCAAAGCCAGGCACAAAGTCTGAGTTTAAAGGCTTTGGGACATCTTC ATCTTTAAATCCAGCACCCTCAGCCTTCAAATTTGGTATTCCATCATCATCTTCTGGGCTTTCTCAAACT TTTACAAGCACTGGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAATTAGGCACTTCATCTGACTCTG GGTCTACAAACACCATGAATACAAACTTTAAATTTCCTAAACCAACTGGAGATTTTAAATTTGGAGTATT ACCTGATTCCAAACCTGAAGAAATAAAAAACGACAGCAAGAATGATAATTTTCAGTTTGGACCTTCTTCT GGCTTAAGCAATCCAGCGTCTTCAGCTCCATTTCAGTTTGGGGTATCTACTCTTGGGCAGCAGGAAAAGA AAGAGGAGCTGCCTCAGTCTTCATCTGCAGGCTTCAGTTTTGGTGCAGGTGTTGCTAACCCCTCTAGTGC TGCTATTGACACCACAGTGACCTCAGAGAACAAGAGCGGCTTCAACTTTGGAACCATAGACACTAAGAGT GTCTCAGTGACTCCTTTCACGTACAAGACAACAGAAGCAAAGAAAGAAGACGCGTCTGCTACCAAGGGTG GGTTCACATTTGGTAAAGTGGACTCTGCTGCTTTGTCATCTCCCTCAATGTTTGTGTTGGGAAGGACAGA GGAGAAACAGCAAGAACCTGTTACTTCTACTTCTCTGGTATTTGGAAAGAAAGCTGACAATGAGGAGCCA AAGTGTCAACCAGTATTTTCCTTTGGAAATTCAGAGCAAACCAAAGACGAGAGTTCCTCCAAGCCGACCT TCAGTTTCAGTGTGGCAAAACCATCTGTGAAGGAGTCTGATCAGCTGGCCAAGGCTACTTTTGCATTTGG AAATCAAACCAATACAACAACTGATCAAGGTGCAGCAAAGCCAGCTTTTAGCTTCTTGAACAGCAGTTCC TCTAGTTCAAGTACGCCGGCCACTTCATCCAGTGCTAGCATATTTGGTAGTTCCACCTCTTCCTCCAGCC CGCCTGTGGCAGCCTTTGTGTTTGGACAGGCCAGTAATCCTGTCAGCAGCTCTGCCTTCGGAAACTCCGC CGAGTCCAGTACATCTCAGCCTTTGCTCTTTCCTCAAGATGGCAAACCAGCCACCACGTCCAGCACTGCC TCAGCTGCCCCTCCATTTGTATTTGGAACAGGAGCCAGTAGTAATAGTACTGTATCTTCTGGGTTCACTT TTGGGGCTACAACCACATCAAGCTCTTCAGGATCCTTCTTTGTATTTGGCACTGGACATTCAGCACCATC TGCCAGTCCAGCATTTGGTGCTAACCAGACTCCAACATTTGGACAAAGTCAAGGTGCCAGCCAGCCTAAC CCTCCAAGCTTTGGCTCTATATCGTCTTCAACAGCATTATTTTCTGCTGGTTCTCAGCCTGTACCACCAC CTACATTTGGGACAGTGTCAAGCAGCAGCCAACCTCCTGTGTTTGGACAGCAGCCTAGTCAGTCTGCATT TGGCTCTGGGACAGCCAATGCTAGTTCGGTTTTCCAGTTTGGCAGCAGCACTACAAATTTCAACTTCACA AACAATAATCCATCAGGAGTGTTCACATTTGGTGCAAGTCCCAGCACGCCTGCAGCCGCAGCCCAGCCCT CAGGTTCGGGGGGCTTTTCATTCAGCCAGTCTCCAGCATCATTTACAGTGGGGTCAAATGGGAAAAATAT GTTCTCTTCTTCCGGAACTTCAGTTTCTGGTCGCAAGATAAAGACTGCTGTTAGACGCAAGAAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213083 representing NM_001100470 Red=Cloning site Green=Tags(s) MASGAGGIGGGGGGGKIRTRRCHQGPVKPYQQGRPQHQGILSRVTESVKNIVPGWLQRYFNKSENACSCS VNADEVPRWPENREDEREIYVDENTNTDDGRTTPEPTGSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFS VLESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASEKDIAVSKNTSLPPLWS PEAERSHSLSQHTAISSKKPAFNLSAFGTLSTSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQNKVR STPYQAPVRRQMKAKQLNAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSAVSSPLNSPLDRSGIDSTV FQAKKEKVDSQYPPVQRLMTPKPVSIATNRTVYFKPSLTPSGDLRKTNQRIDKKNSTVDEKNISRQNREQ ESGFSYPNFSIPAANGLSSGVGGGGGKMRRERTTHFVASKPSEEEEVEVPLLPQISLPISSSSLPTFNFS SPAISAASSSSVSPSQPLSNKVQMTSLGSTGNPVFTFSSPIVKSTQADVLPPASIGFTFSVPLAKTELSG PNSSSETVLSSSVTAQDNTVVNSSSSKKRSAPCEDPFTPAKILREGSVLDILKTPGFMSPKVDSPALQPT TTSSIVYTRPAISTFSSSGVEFGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLPLKETAKQTGIGTP SKSDKPASTSGTGFGDKFKPAIGTWDCDTCLVQNKPEAVKCVACETPKPGTGVKRALPLTVASESPVTAS SSTTVTTGTLGFGDKFKRPVGSWECPVCCVSNKAEDSRCVSCTSEKPGLVSASSSNPVPVSLPSGGCLGL DKFKKPEGSWDCEVCLVQNKADSTKCIACESAKPGTKSEFKGFGTSSSLNPAPSAFKFGIPSSSSGLSQT FTSTGNFKFGDQGGFKLGTSSDSGSTNTMNTNFKFPKPTGDFKFGVLPDSKPEEIKNDSKNDNFQFGPSS GLSNPASSAPFQFGVSTLGQQEKKEELPQSSSAGFSFGAGVANPSSAAIDTTVTSENKSGFNFGTIDTKS VSVTPFTYKTTEAKKEDASATKGGFTFGKVDSAALSSPSMFVLGRTEEKQQEPVTSTSLVFGKKADNEEP KCQPVFSFGNSEQTKDESSSKPTFSFSVAKPSVKESDQLAKATFAFGNQTNTTTDQGAAKPAFSFLNSSS SSSSTPATSSSASIFGSSTSSSSPPVAAFVFGQASNPVSSSAFGNSAESSTSQPLLFPQDGKPATTSSTA SAAPPFVFGTGASSNSTVSSGFTFGATTTSSSSGSFFVFGTGHSAPSASPAFGANQTPTFGQSQGASQPN PPSFGSISSSTALFSAGSQPVPPPTFGTVSSSSQPPVFGQQPSQSAFGSGTANASSVFQFGSSTTNFNFT NNNPSGVFTFGASPSTPAAAAQPSGSGGFSFSQSPASFTVGSNGKNMFSSSGTSVSGRKIKTAVRRKK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100470
ORF Size	4404 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100470.1, NP_001093940.1
RefSeq Size	6224 bp
RefSeq ORF	4407 bp
Locus ID	25281
MW	152.8 kDa
Gene Summary	nuclear pore complex protein; may act to to gate transcribable genes to nuclear pore complexes [RGD, Feb 2006]

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